ID I7MKN2_TETTS Unreviewed; 755 AA.
AC I7MKN2;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 2.
DT 27-MAR-2024, entry version 61.
DE RecName: Full=Protein SEY1 homolog {ECO:0000256|HAMAP-Rule:MF_03109};
DE EC=3.6.5.- {ECO:0000256|HAMAP-Rule:MF_03109};
GN ORFNames=TTHERM_00139640 {ECO:0000313|EMBL:EAR99602.2};
OS Tetrahymena thermophila (strain SB210).
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata;
OC Oligohymenophorea; Hymenostomatida; Tetrahymenina; Tetrahymenidae;
OC Tetrahymena.
OX NCBI_TaxID=312017 {ECO:0000313|EMBL:EAR99602.2, ECO:0000313|Proteomes:UP000009168};
RN [1] {ECO:0000313|Proteomes:UP000009168}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SB210 {ECO:0000313|Proteomes:UP000009168};
RX PubMed=16933976; DOI=10.1371/journal.pbio.0040286;
RA Eisen J.A., Coyne R.S., Wu M., Wu D., Thiagarajan M., Wortman J.R.,
RA Badger J.H., Ren Q., Amedeo P., Jones K.M., Tallon L.J., Delcher A.L.,
RA Salzberg S.L., Silva J.C., Haas B.J., Majoros W.H., Farzad M.,
RA Carlton J.M., Smith R.K. Jr., Garg J., Pearlman R.E., Karrer K.M., Sun L.,
RA Manning G., Elde N.C., Turkewitz A.P., Asai D.J., Wilkes D.E., Wang Y.,
RA Cai H., Collins K., Stewart B.A., Lee S.R., Wilamowska K., Weinberg Z.,
RA Ruzzo W.L., Wloga D., Gaertig J., Frankel J., Tsao C.-C., Gorovsky M.A.,
RA Keeling P.J., Waller R.F., Patron N.J., Cherry J.M., Stover N.A.,
RA Krieger C.J., del Toro C., Ryder H.F., Williamson S.C., Barbeau R.A.,
RA Hamilton E.P., Orias E.;
RT "Macronuclear genome sequence of the ciliate Tetrahymena thermophila, a
RT model eukaryote.";
RL PLoS Biol. 4:1620-1642(2006).
CC -!- FUNCTION: Probable GTP-binding protein involved in generating and
CC maintaining the structure of the tubular endoplasmic reticulum network.
CC {ECO:0000256|ARBA:ARBA00029381}.
CC -!- FUNCTION: Probable GTP-binding protein that may be involved in cell
CC development. {ECO:0000256|HAMAP-Rule:MF_03109}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|HAMAP-Rule:MF_03109}; Multi-pass membrane protein
CC {ECO:0000256|HAMAP-Rule:MF_03109}.
CC -!- SIMILARITY: Belongs to the TRAFAC class dynamin-like GTPase
CC superfamily. GB1/RHD3 GTPase family. RHD3 subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_03109}.
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DR EMBL; GG662639; EAR99602.2; -; Genomic_DNA.
DR RefSeq; XP_001019847.2; XM_001019847.3.
DR AlphaFoldDB; I7MKN2; -.
DR STRING; 312017.I7MKN2; -.
DR GeneID; 7823447; -.
DR KEGG; tet:TTHERM_00139640; -.
DR eggNOG; KOG2203; Eukaryota.
DR InParanoid; I7MKN2; -.
DR OrthoDB; 1606at2759; -.
DR Proteomes; UP000009168; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003924; F:GTPase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR HAMAP; MF_03109; Sey1; 1.
DR InterPro; IPR030386; G_GB1_RHD3_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR008803; RHD3/Sey1.
DR InterPro; IPR046758; Sey1/RHD3-like_3HB.
DR PANTHER; PTHR45923; PROTEIN SEY1; 1.
DR PANTHER; PTHR45923:SF2; PROTEIN SEY1; 1.
DR Pfam; PF05879; RHD3_GTPase; 1.
DR Pfam; PF20428; Sey1_3HB; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51715; G_GB1_RHD3; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|HAMAP-Rule:MF_03109};
KW GTP-binding {ECO:0000256|HAMAP-Rule:MF_03109};
KW Hydrolase {ECO:0000256|HAMAP-Rule:MF_03109};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|HAMAP-Rule:MF_03109};
KW Nucleotide-binding {ECO:0000256|HAMAP-Rule:MF_03109};
KW Reference proteome {ECO:0000313|Proteomes:UP000009168};
KW Transmembrane {ECO:0000256|HAMAP-Rule:MF_03109, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|HAMAP-Rule:MF_03109,
KW ECO:0000256|SAM:Phobius}.
FT TOPO_DOM 1..683
FT /note="Cytoplasmic"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03109"
FT TOPO_DOM 705..707
FT /note="Lumenal"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03109"
FT TRANSMEM 708..728
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TOPO_DOM 729..755
FT /note="Cytoplasmic"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03109"
FT DOMAIN 36..253
FT /note="GB1/RHD3-type G"
FT /evidence="ECO:0000259|PROSITE:PS51715"
FT BINDING 46..53
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_03109"
SQ SEQUENCE 755 AA; 88660 MW; 84AE973FC7BE39AA CRC64;
MSEQSQNQYH HLIDDQCQLI PGLTNFIRQS GLGQSHNSYN IVSIIGSQNS GKSTLLNRVF
GTNFQMLSGS SRTQTTKGIW VSRDKEQNIL ILDVEGSNSR QRGKAEKGSE FYERSTALFA
LAFSQILIIN CNSLNLGHES EYSIIKIIME MNIRLFRSDQ VKQMLIIIRD FNDEVDNFKE
VTESIRNEIY GIWGEIQKPP EFANVQPEQI FKIDFFTSSH FQYQRQKFES DMVQLRDRFI
NPGNPFNLFK GYNYNTNIPI EALEDLATTL WDTIQKNEDL NLPNQKLQAS QVRCLKIKDQ
AIDLINQDLQ KLKQDAESNY LTNFNERAQN IVTRSLHHYD TESQYYVEKI KLEMRKVLND
ELKTALYPIF NQQITSLQQN IKNELKNNLD ALDSRNTSFI KYMDDLSFIR QQIKQVILQG
LHQSLPFNEY KVDWEITKID SQLSKVLEEI INNAREIQFV KYINSQEKTI KNSIDNLISN
CFDTLDLEFW TTIRSGFNQI LKENETRIRD QISQFFDHSR VDKEMNNIIH QSHQFLQDDI
QKKIRELSYY LLKRFKRTFL KDEKGLTRKW KELEEPVIQQ LFLDSRKSIE DILQEFTRFK
VSPPQSHQFS ARMSNMFSSE IVGRAQMNDE VRQAKQSLDF SSEDFSVLLD TSQINKIKQQ
FIDESEEEYN QVLRQRNTSF NTNVPKWMWV VLVFFMYDDV LRWMANPLLL YPIILLGSFI
GLCVATGYQS IPKVLFQQIF GQLTTLLGPL LLKIK
//
