ID   I8RAB4_LACPE            Unreviewed;       678 AA.
AC   I8RAB4;
DT   03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2012, sequence version 1.
DT   24-JAN-2024, entry version 43.
DE   SubName: Full=Transpeptidase (Penicillin binding protein 2B) {ECO:0000313|EMBL:EIW14020.1};
GN   Name=pbp2x {ECO:0000313|EMBL:EIW14020.1};
GN   ORFNames=KCA1_1335 {ECO:0000313|EMBL:EIW14020.1};
OS   Lactiplantibacillus pentosus KCA1.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=1136177 {ECO:0000313|EMBL:EIW14020.1};
RN   [1] {ECO:0000313|EMBL:EIW14020.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KCA1 {ECO:0000313|EMBL:EIW14020.1};
RX   PubMed=23527145;
RA   Anukam K.C., Macklaim J.M., Gloor G.B., Reid G., Boekhorst J., Renckens B.,
RA   van Hijum S.A., Siezen R.J.;
RT   "Genome Sequence of Lactobacillus pentosus KCA1: Vaginal Isolate from a
RT   Healthy Premenopausal Woman.";
RL   PLoS ONE 8:E59239-E59239(2013).
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004162};
CC       Single-pass membrane protein {ECO:0000256|ARBA:ARBA00004162}. Membrane
CC       {ECO:0000256|ARBA:ARBA00004167}; Single-pass membrane protein
CC       {ECO:0000256|ARBA:ARBA00004167}.
CC   -!- SIMILARITY: Belongs to the transpeptidase family.
CC       {ECO:0000256|ARBA:ARBA00007171}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EIW14020.1}.
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DR   EMBL; AKAO01000036; EIW14020.1; -; Genomic_DNA.
DR   RefSeq; WP_003638572.1; NZ_CM001538.1.
DR   AlphaFoldDB; I8RAB4; -.
DR   PATRIC; fig|1136177.4.peg.950; -.
DR   eggNOG; COG0768; Bacteria.
DR   HOGENOM; CLU_009289_7_0_9; -.
DR   Proteomes; UP000005826; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR   Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR   Gene3D; 3.90.1310.10; Penicillin-binding protein 2a (Domain 2); 1.
DR   Gene3D; 1.10.10.1230; Penicillin-binding protein, N-terminal non-catalytic domain, head sub-domain; 1.
DR   InterPro; IPR012338; Beta-lactam/transpept-like.
DR   InterPro; IPR005311; PBP_dimer.
DR   InterPro; IPR036138; PBP_dimer_sf.
DR   InterPro; IPR001460; PCN-bd_Tpept.
DR   PANTHER; PTHR30627; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE; 1.
DR   PANTHER; PTHR30627:SF2; PEPTIDOGLYCAN D,D-TRANSPEPTIDASE MRDA; 1.
DR   Pfam; PF03717; PBP_dimer; 1.
DR   Pfam; PF00905; Transpeptidase; 1.
DR   SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR   SUPFAM; SSF56519; Penicillin binding protein dimerisation domain; 1.
PE   3: Inferred from homology;
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        20..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          66..294
FT                   /note="Penicillin-binding protein dimerisation"
FT                   /evidence="ECO:0000259|Pfam:PF03717"
FT   DOMAIN          343..670
FT                   /note="Penicillin-binding protein transpeptidase"
FT                   /evidence="ECO:0000259|Pfam:PF00905"
SQ   SEQUENCE   678 AA;  72952 MW;  637A37A95F2C1791 CRC64;
     MKFFKKITIN RDPNKSHIPF RLNFLFFIVF LLFAALIAQL AYLQVDYGQK FRSTVNSANN
     TTATGKVQRG SIYDSTGRVL VGNKSHQAIQ YTKGLSVAST KMYEVANKLG EYLTISTSTM
     TDRNLADYYL AGAANLKAVS KKVKTSTDDD VLYAREVAYT EKHIVKNMTD AQKNAAAIYY
     KMNSAYSLST VNIKSTGVTS TELAEIGEHQ SEMPGIKVGT SWTRSYPNGT DLSSVLGTVT
     TEKQGLPSDN IKTLLAEGYS RDDSVGQSQL EKQYENVLSG TKSQTEVKTQ DGVIQKEIKK
     YGGKKGDNIQ LTVNSKFQKK VQQIIMSEAK TVGSANAYSP GAYAVVMNPS TGAIYALAGA
     SRNLSTGKVT ENALGTINQS FVMGSVVKGA TVMGALQDGV ITPTNSTLTD TPIKLAGTAS
     KSSWFNKTGS TSISLNASTA MEVSSNSYMM QLAMKEGNFN YSSGKALTMS NSVFAKLRGY
     FNEFGLGVKT GIDLPGEATG YEGSSAQKDI GKALDLSYGN YDSYTTIQLA QYIATMANGG
     RRIAPHIVSA ITGTKANGSQ GAVKTNVKPR VLNTIDVPSS YFDVVHTGYW DVVHGTSTYK
     TGSALENLSP AVAAKSGTAE TYHGTTSTET LSLVTYAPYK NPKVVIAIAF PGITATSQAY
     NTTTATQIYK AFWKYYNK
//