ID   I9AJK0_LACPE            Unreviewed;       585 AA.
AC   I9AJK0;
DT   03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   03-OCT-2012, sequence version 1.
DT   24-JAN-2024, entry version 42.
DE   SubName: Full=Pyruvate oxidase {ECO:0000313|EMBL:EIW13117.1};
DE            EC=1.2.3.3 {ECO:0000313|EMBL:EIW13117.1};
GN   Name=pox3 {ECO:0000313|EMBL:EIW13117.1};
GN   ORFNames=KCA1_2145 {ECO:0000313|EMBL:EIW13117.1};
OS   Lactiplantibacillus pentosus KCA1.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=1136177 {ECO:0000313|EMBL:EIW13117.1};
RN   [1] {ECO:0000313|EMBL:EIW13117.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KCA1 {ECO:0000313|EMBL:EIW13117.1};
RX   PubMed=23527145;
RA   Anukam K.C., Macklaim J.M., Gloor G.B., Reid G., Boekhorst J., Renckens B.,
RA   van Hijum S.A., Siezen R.J.;
RT   "Genome Sequence of Lactobacillus pentosus KCA1: Vaginal Isolate from a
RT   Healthy Premenopausal Woman.";
RL   PLoS ONE 8:E59239-E59239(2013).
CC   -!- SIMILARITY: Belongs to the TPP enzyme family.
CC       {ECO:0000256|ARBA:ARBA00007812, ECO:0000256|RuleBase:RU362132}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:EIW13117.1}.
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DR   EMBL; AKAO01000068; EIW13117.1; -; Genomic_DNA.
DR   RefSeq; WP_003639362.1; NZ_CM001538.1.
DR   AlphaFoldDB; I9AJK0; -.
DR   PATRIC; fig|1136177.4.peg.2082; -.
DR   eggNOG; COG0028; Bacteria.
DR   HOGENOM; CLU_013748_3_0_9; -.
DR   Proteomes; UP000005826; Chromosome.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR   GO; GO:0047112; F:pyruvate oxidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0030976; F:thiamine pyrophosphate binding; IEA:InterPro.
DR   CDD; cd02014; TPP_POX; 1.
DR   CDD; cd07039; TPP_PYR_POX; 1.
DR   Gene3D; 1.10.10.940; -; 1.
DR   Gene3D; 3.40.50.970; -; 2.
DR   Gene3D; 3.40.50.1220; TPP-binding domain; 1.
DR   InterPro; IPR029035; DHS-like_NAD/FAD-binding_dom.
DR   InterPro; IPR047211; POXB-like.
DR   InterPro; IPR014092; Pyruvate_oxidase.
DR   InterPro; IPR029061; THDP-binding.
DR   InterPro; IPR012000; Thiamin_PyroP_enz_cen_dom.
DR   InterPro; IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
DR   InterPro; IPR000399; TPP-bd_CS.
DR   InterPro; IPR011766; TPP_enzyme_TPP-bd.
DR   InterPro; IPR047212; TPP_POXB-like.
DR   InterPro; IPR047210; TPP_PYR_POXB-like.
DR   NCBIfam; TIGR02720; pyruv_oxi_spxB; 1.
DR   PANTHER; PTHR42981; PYRUVATE DEHYDROGENASE [UBIQUINONE]; 1.
DR   PANTHER; PTHR42981:SF2; PYRUVATE DEHYDROGENASE [UBIQUINONE]; 1.
DR   Pfam; PF02775; TPP_enzyme_C; 1.
DR   Pfam; PF00205; TPP_enzyme_M; 1.
DR   Pfam; PF02776; TPP_enzyme_N; 1.
DR   SUPFAM; SSF52467; DHS-like NAD/FAD-binding domain; 1.
DR   SUPFAM; SSF52518; Thiamin diphosphate-binding fold (THDP-binding); 2.
DR   PROSITE; PS00187; TPP_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Oxidoreductase {ECO:0000313|EMBL:EIW13117.1};
KW   Pyruvate {ECO:0000313|EMBL:EIW13117.1};
KW   Thiamine pyrophosphate {ECO:0000256|RuleBase:RU362132}.
FT   DOMAIN          8..118
FT                   /note="Thiamine pyrophosphate enzyme N-terminal TPP-
FT                   binding"
FT                   /evidence="ECO:0000259|Pfam:PF02776"
FT   DOMAIN          196..324
FT                   /note="Thiamine pyrophosphate enzyme central"
FT                   /evidence="ECO:0000259|Pfam:PF00205"
FT   DOMAIN          386..534
FT                   /note="Thiamine pyrophosphate enzyme TPP-binding"
FT                   /evidence="ECO:0000259|Pfam:PF02775"
SQ   SEQUENCE   585 AA;  64329 MW;  5EDC80D8330CCDB0 CRC64;
     MAKMIEASVA MLKVLEAWDI KQIYGYAGGS FNSTMHALDV EKSRLQYVQV RHEQVGALAA
     AADAKLTGKI GVAFGSAGPG AVNLLNGLYD AKEDHVPVLA LVGQVAHTNM NYDYFQEFAE
     EPIFSDVAVF NRTVMTPESL PYVVDKAIRT AYRENGVAVV TIPNDFGYVE IPDENYDSAS
     VPAKLRGVPE IEDDQVKTVL DMIKAAKYPV FHVGQGTRGA TEDMMALAER LQVPIIITGL
     AKGVIPDSFD GNMGSAYRAA SKAADELMGI TDLVVSVGAD YSFAQIMYTT HDFKYVQIEL
     DPGKFGRHHH LDFGVNGDAK VFIKRALALS EQAAPSPYYQ AAKADMADWK QYLKKLSERT
     TDPLEYEQVY QEINRVAEDD AVFSMDVGDN TINSFRFLKM NPKQKLLTSA LFATMGAGVP
     GAIAAKMSYP DRQAFNIAGD GAFSMVMQDL LTEVKYHLPI FNIVTSNQTL NFIKSEQEDV
     PQPYYGIDLL DADFAKIAEG MGVKGIRVDH FDQLHDAFET AMAELKAGRP VLIDAKITDK
     RGLPVEILDV DPAKTSDASV SAFKEKYDAN ELKPLSSYFK EFKVQ
//