ID I9WJH7_9SPHN Unreviewed; 1163 AA.
AC I9WJH7;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN ORFNames=WSK_1021 {ECO:0000313|EMBL:EIZ80461.1};
OS Novosphingobium sp. Rr 2-17.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Novosphingobium.
OX NCBI_TaxID=555793 {ECO:0000313|EMBL:EIZ80461.1, ECO:0000313|Proteomes:UP000004732};
RN [1] {ECO:0000313|EMBL:EIZ80461.1, ECO:0000313|Proteomes:UP000004732}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RR2-17 {ECO:0000313|Proteomes:UP000004732};
RX PubMed=22933764; DOI=10.1128/JB.01159-12;
RA Gan H.M., Chew T.H., Hudson A.O., Savka M.A.;
RT "Genome Sequence of Novosphingobium sp. Strain Rr 2-17, a Nopaline Crown
RT Gall-Associated Bacterium Isolated from Vitis vinifera L. Grapevine.";
RL J. Bacteriol. 194:5137-5138(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EIZ80461.1}.
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DR EMBL; AKFJ01000012; EIZ80461.1; -; Genomic_DNA.
DR RefSeq; WP_008993976.1; NZ_AKFJ01000012.1.
DR AlphaFoldDB; I9WJH7; -.
DR PATRIC; fig|555793.3.peg.1026; -.
DR eggNOG; COG1074; Bacteria.
DR OrthoDB; 9810135at2; -.
DR Proteomes; UP000004732; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-UniRule.
DR Gene3D; 3.90.320.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 4.
DR InterPro; IPR014151; DNA_helicase_AddA.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR011604; PDDEXK-like_dom_sf.
DR InterPro; IPR038726; PDDEXK_AddAB-type.
DR InterPro; IPR011335; Restrct_endonuc-II-like.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR NCBIfam; TIGR02784; addA_alphas; 1.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF12705; PDDEXK_1; 1.
DR Pfam; PF00580; UvrD-helicase; 2.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF52980; Restriction endonuclease-like; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000004732}.
FT DOMAIN 7..503
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 533..809
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 830..850
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 957..995
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 957..973
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 28..35
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 1163 AA; 126765 MW; 5B2FFDB78BD87E0B CRC64;
MSEPATVHPL HGNQMRAVDP QETVWLSASA GTGKTQVLSS RVLRLLLQPG VEPSQILCLT
FTKAGATEMA ARINGTLAEW VRLGDVPLFQ RLDAIGAPAD PATIARARTL FAAVLDCPGG
GLRIDTIHAF SQWLLATFPL EADLVPGSRP MEDRERELLA RQVLADLLVS AQGDPLGDPA
LLKAVEVLSL RHGPDAVVAF LLRCANARDA WFGPGSWQAG DMRANVLRLI GLPPEADADW
LAEMCTDAAF DVASLRHCMD VNHRWGTKTG LAAVDAISEW LLGDPAQRFS GIAALAGVFL
TKTGSPRASA SQEKLDPAYP DYRDRVIERI AAITSAKALL ELADRLVPAL RLGRAFALAW
DEAKQREGLI DFDDQIRRAA SLLGDKEQAD WIRYKLDRRF DHIMVDEAQD TNAQQWAIIL
AMASEFWAGL GQRADLMRTL FVVGDYKQAI FRFQGTSPEN FRRAAERVRR DMHQAAQNAE
LLRTNHSPRR LLELGLDRSF RTAQHVLDFV DDAIAGVGHE SFGLDRPPER HVGQDRPGYV
ALWKPVSAFS DIEDDLSEDE EGTSPDWLSR PDRQMAERIA LQVSEWLDEG GPGLALAKGT
PRRAEAGDVM VLVRQRKELA SLIVARLHAA GVPVAGVDRL RLGAPLAVKD LVAALRFAVQ
PLDDLNLAAL LVSPLVGWSQ EQLLRHAWRE PRLRLWEHLR ASTDPEVGAV LIQLGGLLTR
ADFEMPHETL HWLLVGPWQG RRRLVARLGS EANDPIDELI NAARAYVVTD TPSLAGFLSW
FDAGDGELKR EADAASGLVR VMTVHGSKGL QAPIVILADA AGNPDNARDR GLDLPDPANE
DRTIPLPPLG KAEKQGRIAA KIEADRLADE QEYWRLLYVA MTRAEECLFV GGALTSRDKG
EPSPKSWYAR LRALFAPEAE VADSIWGARY EHGALPPPVP VTPKALTLPL RDPLPPWLER
APAAEPRPPR PLAPSSLGEE DAPDPPFPPG SGREAARRGT LIHRLLERLP DVSPDARDAA
GEAWLARHAF DMSETDRSAL LAGALAVIGN PEWADLFAPG SLAEVPLAAV VGGQVISGTI
DRLVVTPTHV RLVDYKTARR PPASLEEMPK GVLRQMGAYA AALQATFPGR AVEVAVLYTA
TPRLIVVPGD VLADYKPTLA ASQ
//
