ID INSL3_RAT Reviewed; 128 AA.
AC Q9WUK0; Q9WUK1;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2003, sequence version 2.
DT 24-JAN-2024, entry version 145.
DE RecName: Full=Insulin-like 3;
DE AltName: Full=Leydig insulin-like peptide;
DE Short=Ley-I-L;
DE AltName: Full=Relaxin-like factor;
DE Contains:
DE RecName: Full=Insulin-like 3 B chain;
DE Contains:
DE RecName: Full=Insulin-like 3 A chain;
DE Flags: Precursor;
GN Name=Insl3; Synonyms=Rlf;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=10542371;
RX DOI=10.1002/(sici)1098-2795(199912)54:4<319::aid-mrd1>3.0.co;2-z;
RA Spiess A.-N., Balvers M., Tena-Sempere M., Huhtaniemi I., Parry L.,
RA Ivell R.;
RT "Structure and expression of the rat relaxin-like factor (RLF) gene.";
RL Mol. Reprod. Dev. 54:319-325(1999).
CC -!- FUNCTION: Seems to play a role in testicular function. May be a trophic
CC hormone with a role in testicular descent in fetal life. Is a ligand
CC for LGR8 receptor (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two
CC disulfide bonds. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- TISSUE SPECIFICITY: Expressed in Leydig cells of the testis, and weakly
CC in the theca interna cells of antral follicles and the corpus luteum of
CC the ovary. {ECO:0000269|PubMed:10542371}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed in adult testis and low
CC expression in the ovary. Highly up-regulated in testes of day 19
CC embryos, but not in later neonatal stages, nor any ovarian tissue from
CC this period. {ECO:0000269|PubMed:10542371}.
CC -!- SIMILARITY: Belongs to the insulin family. {ECO:0000305}.
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DR EMBL; AF139918; AAD33663.2; -; mRNA.
DR EMBL; AF139920; AAD33851.1; -; Genomic_DNA.
DR RefSeq; NP_446132.1; NM_053680.1.
DR AlphaFoldDB; Q9WUK0; -.
DR SMR; Q9WUK0; -.
DR STRING; 10116.ENSRNOP00000025349; -.
DR PaxDb; 10116-ENSRNOP00000025349; -.
DR Ensembl; ENSRNOT00000080146.2; ENSRNOP00000073659.2; ENSRNOG00000068505.1.
DR Ensembl; ENSRNOT00055016310; ENSRNOP00055013109; ENSRNOG00055009650.
DR Ensembl; ENSRNOT00060019730; ENSRNOP00060015527; ENSRNOG00060011653.
DR Ensembl; ENSRNOT00065035501; ENSRNOP00065028608; ENSRNOG00065020905.
DR GeneID; 114215; -.
DR KEGG; rno:114215; -.
DR UCSC; RGD:620117; rat.
DR AGR; RGD:620117; -.
DR CTD; 3640; -.
DR RGD; 620117; Insl3.
DR eggNOG; ENOG502TFQI; Eukaryota.
DR GeneTree; ENSGT00940000163613; -.
DR HOGENOM; CLU_164865_0_0_1; -.
DR InParanoid; Q9WUK0; -.
DR OMA; NPAHHCC; -.
DR OrthoDB; 5310508at2759; -.
DR PhylomeDB; Q9WUK0; -.
DR TreeFam; TF106361; -.
DR Reactome; R-RNO-444821; Relaxin receptors.
DR PRO; PR:Q9WUK0; -.
DR Proteomes; UP000002494; Chromosome 16.
DR Bgee; ENSRNOG00000018757; Expressed in testis and 11 other cell types or tissues.
DR Genevisible; Q9WUK0; RN.
DR GO; GO:0005615; C:extracellular space; IDA:RGD.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
DR GO; GO:0001664; F:G protein-coupled receptor binding; IPI:RGD.
DR GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR GO; GO:0002020; F:protease binding; ISO:RGD.
DR GO; GO:0007193; P:adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway; IDA:RGD.
DR GO; GO:0001701; P:in utero embryonic development; ISO:RGD.
DR GO; GO:0008584; P:male gonad development; IDA:RGD.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IDA:RGD.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; IDA:RGD.
DR GO; GO:0001556; P:oocyte maturation; IDA:RGD.
DR GO; GO:0043950; P:positive regulation of cAMP-mediated signaling; IDA:RGD.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:RGD.
DR GO; GO:0010634; P:positive regulation of epithelial cell migration; ISO:RGD.
DR GO; GO:0090303; P:positive regulation of wound healing; ISO:RGD.
DR GO; GO:2000018; P:regulation of male gonad development; ISO:RGD.
DR CDD; cd04365; IlGF_relaxin_like; 1.
DR Gene3D; 1.10.100.10; Insulin-like; 1.
DR InterPro; IPR043387; INSL3/INSL4.
DR InterPro; IPR016179; Insulin-like.
DR InterPro; IPR036438; Insulin-like_sf.
DR InterPro; IPR022353; Insulin_CS.
DR InterPro; IPR022352; Insulin_family.
DR PANTHER; PTHR10423; INSULIN-LIKE 3; 1.
DR PANTHER; PTHR10423:SF3; INSULIN-LIKE 3; 1.
DR Pfam; PF00049; Insulin; 1.
DR PRINTS; PR00276; INSULINFAMLY.
DR SMART; SM00078; IlGF; 1.
DR SUPFAM; SSF56994; Insulin-like; 1.
DR PROSITE; PS00262; INSULIN; 1.
PE 2: Evidence at transcript level;
KW Cleavage on pair of basic residues; Disulfide bond; Hormone;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..15
FT /evidence="ECO:0000255"
FT PEPTIDE 16..?
FT /note="Insulin-like 3 B chain"
FT /id="PRO_0000016152"
FT PROPEP ?..100
FT /note="C peptide like"
FT /evidence="ECO:0000255"
FT /id="PRO_0000016153"
FT PEPTIDE 103..128
FT /note="Insulin-like 3 A chain"
FT /id="PRO_0000016154"
FT REGION 81..101
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 29..113
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 41..126
FT /note="Interchain (between B and A chains)"
FT /evidence="ECO:0000250"
FT DISULFID 112..117
FT /evidence="ECO:0000250"
SQ SEQUENCE 128 AA; 14110 MW; ABF2EE3AE30ADD53 CRC64;
MHALLLLLLL ALGSALRSPQ PPEARAKLCG HHLVRALVRV CGGPRWSPEA TQPVDTRDRE
LLQWLEQRHL LHALVADADP ALDPDPALDP QLPHQASQRQ RRSVATNAVH RCCLTGCTQQ
DLLGLCPH
//
