ID INTS_ECOLI Reviewed; 385 AA.
AC P37326; P76942;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 27-MAR-2024, entry version 146.
DE RecName: Full=Prophage integrase IntS {ECO:0000305};
DE AltName: Full=Putative prophage CPS-53 integrase;
GN Name=intS; Synonyms=intC, yfdB; OrderedLocusNames=b2349, JW2345;
OS Escherichia coli (strain K12).
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RA Baumann S.;
RL Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5] {ECO:0007744|PDB:2KJ8}
RP STRUCTURE BY NMR OF 87-196.
RA Cort J.R., Montelione G.T., Kennedy M.A.;
RT "NMR structure of fragment 87-196 from the putative phage integrase IntS of
RT E. coli.";
RL Submitted (MAY-2009) to the PDB data bank.
CC -!- FUNCTION: Integrase is necessary for integration of the phage into the
CC host genome by site-specific recombination. In conjunction with
CC excisionase, integrase is also necessary for excision of the prophage
CC from the host genome.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. {ECO:0000305}.
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DR EMBL; U11296; AAA19609.1; -; Unassigned_DNA.
DR EMBL; U00096; AAC75408.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA16208.1; -; Genomic_DNA.
DR PIR; I84552; I84552.
DR RefSeq; NP_416850.1; NC_000913.3.
DR RefSeq; WP_000958671.1; NZ_LN832404.1.
DR PDB; 2KJ8; NMR; -; A=87-196.
DR PDBsum; 2KJ8; -.
DR AlphaFoldDB; P37326; -.
DR SMR; P37326; -.
DR BioGRID; 4259384; 12.
DR DIP; DIP-10041N; -.
DR IntAct; P37326; 12.
DR STRING; 511145.b2349; -.
DR PaxDb; 511145-b2349; -.
DR EnsemblBacteria; AAC75408; AAC75408; b2349.
DR GeneID; 946821; -.
DR KEGG; ecj:JW2345; -.
DR KEGG; eco:b2349; -.
DR PATRIC; fig|511145.12.peg.2445; -.
DR EchoBASE; EB2312; -.
DR eggNOG; COG0582; Bacteria.
DR HOGENOM; CLU_027562_0_0_6; -.
DR InParanoid; P37326; -.
DR OMA; WSEGYAK; -.
DR OrthoDB; 9795573at2; -.
DR PhylomeDB; P37326; -.
DR BioCyc; EcoCyc:G7218-MONOMER; -.
DR EvolutionaryTrace; P37326; -.
DR PRO; PR:P37326; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0042802; F:identical protein binding; IDA:EcoCyc.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:EcoCyc.
DR GO; GO:0015074; P:DNA integration; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0075713; P:establishment of integrated proviral latency; IEA:UniProtKB-KW.
DR GO; GO:0032359; P:provirus excision; IMP:EcoCyc.
DR GO; GO:0046718; P:viral entry into host cell; IEA:UniProtKB-KW.
DR GO; GO:0044826; P:viral genome integration into host DNA; IEA:UniProtKB-KW.
DR CDD; cd00801; INT_P4_C; 1.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 3.30.160.390; Integrase, DNA-binding domain; 1.
DR Gene3D; 1.10.443.10; Intergrase catalytic core; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR038488; Integrase_DNA-bd_sf.
DR InterPro; IPR025166; Integrase_DNA_bind_dom.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR PANTHER; PTHR30629; PROPHAGE INTEGRASE; 1.
DR PANTHER; PTHR30629:SF2; PROPHAGE INTEGRASE INTS-RELATED; 1.
DR Pfam; PF13356; Arm-DNA-bind_3; 1.
DR Pfam; PF14659; Phage_int_SAM_3; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA integration; DNA recombination; DNA-binding;
KW Reference proteome; Viral genome integration; Virus entry into host cell.
FT CHAIN 1..385
FT /note="Prophage integrase IntS"
FT /id="PRO_0000197518"
FT DOMAIN 91..172
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01248"
FT DOMAIN 195..373
FT /note="Tyr recombinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 234
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 261
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 324
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 327
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 350
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT ACT_SITE 360
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01246"
FT HELIX 93..104
FT /evidence="ECO:0007829|PDB:2KJ8"
FT TURN 105..107
FT /evidence="ECO:0007829|PDB:2KJ8"
FT HELIX 110..128
FT /evidence="ECO:0007829|PDB:2KJ8"
FT HELIX 138..149
FT /evidence="ECO:0007829|PDB:2KJ8"
FT TURN 150..152
FT /evidence="ECO:0007829|PDB:2KJ8"
FT HELIX 154..173
FT /evidence="ECO:0007829|PDB:2KJ8"
FT HELIX 182..191
FT /evidence="ECO:0007829|PDB:2KJ8"
FT HELIX 192..194
FT /evidence="ECO:0007829|PDB:2KJ8"
SQ SEQUENCE 385 AA; 44061 MW; C3D9B1C59A06DBBA CRC64;
MLTVKQIEAA KPKEKPYRLL DGNGLYLYVP VSGKKVWQLR YKIDGKEKIL TVGKYPLMTL
QEARDKAWTA RKDISVGIDP VKAKKASSNN NSFSAIYKEW YEHKKQVWSV GYATELAKMF
DDDILPIIGG LEIQDIEPMQ LLEVIRRFED RGAMERANKA RRRCGEVFRY AIVTGRAKYN
PAPDLADAMK GYRKKNFPFL PADQIPAFNK ALATFSGSIV SLIATKVLRY TALRTKELRS
MLWKNVDFEN RIITIDASVM KGRKIHVVPM SDQVVELLTT LSSITKPVSE FVFAGRNDKK
KPICENAVLL VIKQIGYEGL ESGHGFRHEF STIMNEHEWP ADAIEVQLAH ANGGSVRGIY
NHAQYLDKRR EMMQWWADWL DEKVE
//
