ID ISK8_MOUSE Reviewed; 105 AA.
AC Q09TK9;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 1.
DT 24-JAN-2024, entry version 101.
DE RecName: Full=Serine protease inhibitor Kazal-type 8;
DE Flags: Precursor;
GN Name=Spink8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=FVB/N; TISSUE=Epididymis;
RX PubMed=16930550; DOI=10.1016/j.bbrc.2006.08.023;
RA Jalkanen J., Kotimaeki M., Huhtaniemi I., Poutanen M.;
RT "Novel epididymal protease inhibitors with Kazal or WAP family domain.";
RL Biochem. Biophys. Res. Commun. 349:245-254(2006).
CC -!- FUNCTION: Probable serine protease inhibitor.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in epydiymis, in the cauda, corpus and
CC caput. {ECO:0000269|PubMed:16930550}.
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DR EMBL; DQ437329; ABD93324.1; -; mRNA.
DR CCDS; CCDS40777.1; -.
DR RefSeq; NP_898959.2; NM_183136.2.
DR RefSeq; XP_006512461.1; XM_006512398.3.
DR AlphaFoldDB; Q09TK9; -.
DR SMR; Q09TK9; -.
DR STRING; 10090.ENSMUSP00000142586; -.
DR PaxDb; 10090-ENSMUSP00000052529; -.
DR ProteomicsDB; 269338; -.
DR Antibodypedia; 56531; 77 antibodies from 15 providers.
DR DNASU; 78709; -.
DR Ensembl; ENSMUST00000198988.5; ENSMUSP00000142586.2; ENSMUSG00000050074.13.
DR GeneID; 78709; -.
DR KEGG; mmu:78709; -.
DR UCSC; uc009rsr.1; mouse.
DR AGR; MGI:1925959; -.
DR CTD; 646424; -.
DR MGI; MGI:1925959; Spink8.
DR VEuPathDB; HostDB:ENSMUSG00000050074; -.
DR eggNOG; ENOG502TDVY; Eukaryota.
DR GeneTree; ENSGT00520000060726; -.
DR InParanoid; Q09TK9; -.
DR OMA; YDGPCEN; -.
DR PhylomeDB; Q09TK9; -.
DR BioGRID-ORCS; 78709; 4 hits in 77 CRISPR screens.
DR ChiTaRS; Spink8; mouse.
DR PRO; PR:Q09TK9; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q09TK9; Protein.
DR Bgee; ENSMUSG00000050074; Expressed in seminal vesicle and 60 other cell types or tissues.
DR ExpressionAtlas; Q09TK9; baseline and differential.
DR Genevisible; Q09TK9; MM.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR Gene3D; 3.30.60.30; -; 1.
DR InterPro; IPR002350; Kazal_dom.
DR InterPro; IPR036058; Kazal_dom_sf.
DR PANTHER; PTHR21312; SERINE PROTEASE INHIBITOR; 1.
DR PANTHER; PTHR21312:SF37; SERINE PROTEASE INHIBITOR KAZAL-TYPE 8; 1.
DR Pfam; PF00050; Kazal_1; 1.
DR SMART; SM00280; KAZAL; 1.
DR SUPFAM; SSF100895; Kazal-type serine protease inhibitors; 1.
DR PROSITE; PS51465; KAZAL_2; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Repeat; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..105
FT /note="Serine protease inhibitor Kazal-type 8"
FT /id="PRO_0000302140"
FT DOMAIN 44..98
FT /note="Kazal-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT SITE 53..54
FT /note="Reactive bond"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 44..78
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 51..75
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT DISULFID 64..96
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
SQ SEQUENCE 105 AA; 11778 MW; 8A9DA02483C15A8C CRC64;
MKVIFSVAVL VLASSVWTSL AVDFILPMNF HMTGELLQKT KALCIKNIQL CWILSYFKVS
EPICGSNQVT YEGECHLCSG ILYEDRTVIK VHDGPCEHSS DESEH
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