ID J0LND9_9BACT Unreviewed; 735 AA.
AC J0LND9;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Penicillin amidase family protein {ECO:0000313|EMBL:EJF10958.1};
GN ORFNames=O71_06162 {ECO:0000313|EMBL:EJF10958.1};
OS Pontibacter sp. BAB1700.
OC Bacteria; Bacteroidota; Cytophagia; Cytophagales; Hymenobacteraceae;
OC Pontibacter.
OX NCBI_TaxID=1144253 {ECO:0000313|EMBL:EJF10958.1, ECO:0000313|Proteomes:UP000003746};
RN [1] {ECO:0000313|EMBL:EJF10958.1, ECO:0000313|Proteomes:UP000003746}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BAB1700 {ECO:0000313|EMBL:EJF10958.1,
RC ECO:0000313|Proteomes:UP000003746};
RX PubMed=23105068; DOI=10.1128/JB.01550-12;
RA Joshi M.N., Sharma A.C., Pandya R.V., Patel R.P., Saiyed Z.M., Saxena A.K.,
RA Bagatharia S.B.;
RT "Draft Genome Sequence of Pontibacter sp. nov. BAB1700, a Halotolerant,
RT Industrially Important Bacterium.";
RL J. Bacteriol. 194:6329-6330(2012).
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJF10958.1}.
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DR EMBL; AKIS01000038; EJF10958.1; -; Genomic_DNA.
DR RefSeq; WP_007654067.1; NZ_AKIS01000038.1.
DR AlphaFoldDB; J0LND9; -.
DR MEROPS; S45.002; -.
DR PATRIC; fig|1144253.3.peg.1217; -.
DR Proteomes; UP000003746; Unassembled WGS sequence.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd01936; Ntn_CA; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Signal {ECO:0000256|SAM:SignalP}; Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT SIGNAL 1..22
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 23..735
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003735893"
FT ACT_SITE 210
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 280
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 283
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 735 AA; 83092 MW; C6BA8BDE1F24B4E8 CRC64;
MNYLFRPQVF LLLLFLFVSS CATNPKTDPA SEVAKWEEQA ARVTIMRDDF GVPHIYGKTD
ADAVFGLLYA QCEDDFNRVE RNYMWAIGRL SEVEGEEQLY SDLRARLYMT EEEAKAEYER
SPAWLKELCQ AFADGINYYL HTHPEVKPKL LTRFEPWMPM YFSEGSIGGD IESVSTKGIK
AFYEEDRSLG LNSQGHGLVQ PALLEEPKGS NGFAISGKHT ASGDAMLLIN PHTSFFFRGE
VHAVSEEGLN AYGAVTWGQF FIYQGFNEKT GWMHTSAYAD VIDEFEETIV RQDGKLFYRY
GEELRPVETK EVTLAYKDGG ELKEKTFTTY RTHHGPITHM NGDKWVATAL MWKPVDALIQ
SYTRTKKSNL KEFNEMMQMR TNSSNATVYA DADGNIAYYH GNFFPKRDTS FDYSKPVDGS
NPKTDWNGLH TLDETIRIMN PPNGWIQNCN STPYTAAAEY SPKKEDYPVY MAPSPENFRG
VHAIRLLKKA ENLTLDKLID LAYDPYLPGF EVLIPGLVKA YDAQKPNDPK LKAAIDVMRN
WDYAVSKESV AMSLAHFYAT NLLRDGSAPK GHIMERFDYY ANTAPAAERL DIFSKTITEL
EQDFGQWNTP WGEINRFQRL TGDIKQPFND AAPSIPVGMA SGNWGALASY GANRYDTKRL
YGTSGNSFVA VVEFGDKVKA KTILAGGQSG DPSSPHFNDQ AARYANVEFK DVAYYREDVE
KRAKATYKPG QKQSK
//
