ID J2CRB3_9SPHN Unreviewed; 1190 AA.
AC J2CRB3;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=Error-prone DNA polymerase {ECO:0000256|ARBA:ARBA00017273, ECO:0000256|HAMAP-Rule:MF_01902};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|HAMAP-Rule:MF_01902};
GN Name=dnaE2 {ECO:0000256|HAMAP-Rule:MF_01902};
GN ORFNames=PMI04_03770 {ECO:0000313|EMBL:EJK79544.1};
OS Sphingobium sp. AP49.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingobium.
OX NCBI_TaxID=1144307 {ECO:0000313|EMBL:EJK79544.1};
RN [1] {ECO:0000313|EMBL:EJK79544.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AP49 {ECO:0000313|EMBL:EJK79544.1};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- FUNCTION: DNA polymerase involved in damage-induced mutagenesis and
CC translesion synthesis (TLS). It is not the major replicative DNA
CC polymerase. {ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632, ECO:0000256|HAMAP-
CC Rule:MF_01902};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_01902}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-C family. DnaE2
CC subfamily. {ECO:0000256|ARBA:ARBA00007391, ECO:0000256|HAMAP-
CC Rule:MF_01902}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJK79544.1}.
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DR EMBL; AJVL01000076; EJK79544.1; -; Genomic_DNA.
DR RefSeq; WP_007713663.1; NZ_CP124576.1.
DR AlphaFoldDB; J2CRB3; -.
DR STRING; 1144307.PMI04_03770; -.
DR PATRIC; fig|1144307.3.peg.3520; -.
DR eggNOG; COG0587; Bacteria.
DR OrthoDB; 9803237at2; -.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04485; DnaE_OBF; 1.
DR CDD; cd07434; PHP_PolIIIA_DnaE2; 1.
DR Gene3D; 1.10.150.870; -; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_01902; DNApol_error_prone; 1.
DR InterPro; IPR011708; DNA_pol3_alpha_NTPase_dom.
DR InterPro; IPR040982; DNA_pol3_finger.
DR InterPro; IPR023073; DnaE2.
DR InterPro; IPR004805; DnaE2/DnaE/PolC.
DR InterPro; IPR029460; DNAPol_HHH.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR InterPro; IPR004013; PHP_dom.
DR InterPro; IPR003141; Pol/His_phosphatase_N.
DR NCBIfam; TIGR00594; polc; 1.
DR PANTHER; PTHR32294; DNA POLYMERASE III SUBUNIT ALPHA; 1.
DR PANTHER; PTHR32294:SF4; ERROR-PRONE DNA POLYMERASE; 1.
DR Pfam; PF07733; DNA_pol3_alpha; 1.
DR Pfam; PF17657; DNA_pol3_finger; 1.
DR Pfam; PF14579; HHH_6; 1.
DR Pfam; PF02811; PHP; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR SMART; SM00481; POLIIIAc; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_01902};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_01902};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_01902};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_01902}.
FT DOMAIN 46..144
FT /note="Polymerase/histidinol phosphatase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00481"
FT REGION 1159..1190
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1190 AA; 132887 MW; C11D7260747D2168 CRC64;
MSGRRPPPDP AKPRSIDRRE LNAIEAAREK TKPALPRSAP AEPPFAELVA ISNYSFLHGA
SHPADLVGEA IALGFKAIGV ADRNSVAGVV RAYVALRDAP ETARTKLLED KKARGEPESL
TPEEEAGCET DLRLIVGARL VFVDGTPDII VYPASRQGWG KLTRLLTKGN IKAIKGDCIL
HFADLLDYLD DWRMIILPLS TAQAQQEHDA PPDYAFEDDA APVPSRHLRL VPPASPPSWQ
EAARQLQERM PDRVWLGLSM RYQGRDARHV AMMQAVSRDI GIPLIATNDI LYATSGTRPL
QDVLTCIREG VTLKTAGTRL QANAERHMKS PTEMARLFAV CPDALAETIR FIDGIDFTLK
DLAYEYPHEP VPAGWSPDAW LEDMVRRRAR RRYGRQLPPK VRALIGQELR LIRRQNYAYY
FLTVHDLVRF ARAQKPPILC QGRGSAANSI VCFLLGVTSI DPMEHDLLFS RFVSEERNEP
PDIDVDFEHE RREEVIQYIY RRYGRARAGI AATVIHYRSR SALREVAKVL GLSEDVVMRL
SATVWGSYSG DLGDARFVDA GFALDNGEIQ RLRTLVQQLI KAPFPRHLSQ HVGGFVLTQD
RLDETVPIHN AAMADRSFIE WDKDDIDALN LMKVDVLALG MLSCIRRAYA LMNQHGLGRH
DLTVDLQSRD PAVYDMLCKG DSIGVFQVES RAQINMLPRL KPRNLYDLTV QVAIVRPGPI
EGDMVHPYLR RRCGEEKVEY PDPDIAFGPP DELKNLLKDT YGVPLFQEQA MKLAIVAAGF
SSSEANQLRR AMATFRNVGT IHHFREKMIN GMTARGYEAE FAARCFKQIE GFGSYGFPES
HALSFARLVY VSAWIKCHQP AIFACALLNS QPMGFYAPAQ LVRDARDHGV AIHDVDVNMS
DWDNGLEPLL RSRRADKGEG SGRALALRLG LRQVDGFREQ WAAQMVAARA EGGLFTSIED
MARRASLPAR ALRLLADADA CRSLGQDRRT ALWEARRTPS DELPLFAAAR RHDRAARELG
EEADAMLPAM PLAEQVMDDY AVTRLSLKGH PMQFLRPVFA AEGVLSCAQT SAAKNGTRVR
TAGVVLVRQR PGKGNAVFIT IEDETGITNI LLWARLFELQ RRPVMASRLM LVEGEVQRSK
EGVVHLMASR IHDRTADLNQ LSHGKPSQAT PAAARHPRDV RILPKSRDFH
//
