ID J2UWT2_9BURK Unreviewed; 812 AA.
AC J2UWT2;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=DNA polymerase {ECO:0000256|RuleBase:RU000442};
DE EC=2.7.7.7 {ECO:0000256|RuleBase:RU000442};
GN ORFNames=PMI40_04218 {ECO:0000313|EMBL:EJM97736.1};
OS Herbaspirillum sp. YR522.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Herbaspirillum.
OX NCBI_TaxID=1144342 {ECO:0000313|EMBL:EJM97736.1, ECO:0000313|Proteomes:UP000007507};
RN [1] {ECO:0000313|EMBL:EJM97736.1, ECO:0000313|Proteomes:UP000007507}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YR522 {ECO:0000313|EMBL:EJM97736.1,
RC ECO:0000313|Proteomes:UP000007507};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU000442};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755, ECO:0000256|RuleBase:RU000442}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJM97736.1}.
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DR EMBL; AKJA01000134; EJM97736.1; -; Genomic_DNA.
DR RefSeq; WP_008118778.1; NZ_AKJA01000134.1.
DR AlphaFoldDB; J2UWT2; -.
DR STRING; 1144342.PMI40_04218; -.
DR PATRIC; fig|1144342.3.peg.3840; -.
DR eggNOG; COG0417; Bacteria.
DR OrthoDB; 5807460at2; -.
DR Proteomes; UP000007507; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd05784; DNA_polB_II_exo; 1.
DR CDD; cd05537; POLBc_Pol_II; 1.
DR Gene3D; 2.40.50.590; -; 2.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 2.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR017964; DNA-dir_DNA_pol_B_CS.
DR InterPro; IPR006133; DNA-dir_DNA_pol_B_exonuc.
DR InterPro; IPR006134; DNA-dir_DNA_pol_B_multi_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10322; DNA POLYMERASE CATALYTIC SUBUNIT; 1.
DR PANTHER; PTHR10322:SF23; DNA POLYMERASE DELTA CATALYTIC SUBUNIT; 1.
DR Pfam; PF00136; DNA_pol_B; 1.
DR Pfam; PF03104; DNA_pol_B_exo1; 1.
DR Pfam; PF21474; DNApolII_N; 1.
DR PRINTS; PR00106; DNAPOLB.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00116; DNA_POLYMERASE_B; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|RuleBase:RU000442};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU000442};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU000442};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU000442};
KW Reference proteome {ECO:0000313|Proteomes:UP000007507};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU000442}.
FT DOMAIN 103..323
FT /note="DNA-directed DNA polymerase family B exonuclease"
FT /evidence="ECO:0000259|Pfam:PF03104"
FT DOMAIN 403..789
FT /note="DNA-directed DNA polymerase family B
FT multifunctional"
FT /evidence="ECO:0000259|Pfam:PF00136"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 812 AA; 91404 MW; 1A132B75621D7876 CRC64;
MTGIAVQRQD IGPIDEQPPS HPQPVPECAG FLLTRHWRDT PAGVEIELWV ATDDGPRRLL
LAPQEAVAFI RTQDLERAQA LLAGEPGVAL RALPGLKDFA QHPVLGLYSN QYRKLLKAEK
QLMQSGITVF EADIRPPERY LMERFINAPL LLSGLPGADG SLHQARVRAH PDYRPPLRLV
SLDIETSWQG ELYSIALEGC GQRTVYVLGT APQQGAAPDF ALHFCDSRAE LIACLNRWMA
RHDPDAIIGW NLIQFDLQVL QKQADKYGVP LQLGRGGRKL EWREHGFRAN HMLVTAPGRL
MIDGIESLRS AFWSFPSFSL ENVAQTLLGE GKAIATPYQR MDEIDRMFAE DRVALARYNL
KDCELVTRIF AHTGILAFLL ERAAVTGLAV DRSGGSVAAF THLYLPQMHR KGYVAPNLGD
AEAIASPGGF VMESRPGLYD SVLVLDYKSL YPAIIRSFLI DPVGLVEGTL VEPEADTVPG
FNQARFSRRS HSLPAIIAQI WQGRDDAKRQ GNQPLSQALK IIMNSFYGVL GSTACRFFDS
RLASSITRRG HEIMHRTREL IEAQGFEVIY GDTDSTFVWL KRAHDDPSAR AIGIELTRTI
NAWWRQHLQV QYGLDSALEL QFEVHYQRFL MPSVRGAEIG SKKRYAGYVR DAQGGDRVTY
KGLETVRTDW TPLAQQFQQA LYLRIFRGEP YRDYIVGYVA DLLAGRLDEQ LIYRKRLRRQ
LDHYEKNVPP HVRAARLADQ VNRDDGRPLQ YQQGGWINYV MTTAGPEPLE KRSAAIDYGH
YLERQLAPVA DAILMFVDDS FARITSPQQA LF
//
