ID J3C7G2_FLASC Unreviewed; 567 AA.
AC J3C7G2;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE SubName: Full=Por secretion system C-terminal sorting domain-containing protein {ECO:0000313|EMBL:EJL65821.1};
GN ORFNames=PMI10_01128 {ECO:0000313|EMBL:EJL65821.1};
OS Flavobacterium sp. (strain CF136).
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=1144313 {ECO:0000313|EMBL:EJL65821.1, ECO:0000313|Proteomes:UP000007287};
RN [1] {ECO:0000313|EMBL:EJL65821.1, ECO:0000313|Proteomes:UP000007287}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CF136 {ECO:0000313|EMBL:EJL65821.1,
RC ECO:0000313|Proteomes:UP000007287};
RX PubMed=23045501; DOI=10.1128/JB.01243-12;
RA Brown S.D., Utturkar S.M., Klingeman D.M., Johnson C.M., Martin S.L.,
RA Land M.L., Lu T.Y., Schadt C.W., Doktycz M.J., Pelletier D.A.;
RT "Twenty-one genome sequences from Pseudomonas species and 19 genome
RT sequences from diverse bacteria isolated from the rhizosphere and
RT endosphere of Populus deltoides.";
RL J. Bacteriol. 194:5991-5993(2012).
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJL65821.1}.
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DR EMBL; AKJZ01000012; EJL65821.1; -; Genomic_DNA.
DR AlphaFoldDB; J3C7G2; -.
DR STRING; 1144313.PMI10_01128; -.
DR PATRIC; fig|1144313.4.peg.1126; -.
DR eggNOG; COG3507; Bacteria.
DR OrthoDB; 9757947at2; -.
DR Proteomes; UP000007287; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd08998; GH43_Arb43a-like; 1.
DR CDD; cd00161; RICIN; 1.
DR Gene3D; 2.80.10.50; -; 2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR035992; Ricin_B-like_lectins.
DR InterPro; IPR000772; Ricin_B_lectin.
DR InterPro; IPR026444; Secre_tail.
DR NCBIfam; TIGR04183; Por_Secre_tail; 1.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR Pfam; PF18962; Por_Secre_tail; 1.
DR Pfam; PF14200; RicinB_lectin_2; 2.
DR SMART; SM00458; RICIN; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF50370; Ricin B-like lectins; 1.
DR PROSITE; PS50231; RICIN_B_LECTIN; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Membrane {ECO:0000256|SAM:Phobius}; Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 20..42
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 326..458
FT /note="Ricin B lectin"
FT /evidence="ECO:0000259|SMART:SM00458"
FT ACT_SITE 48
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 209
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 162
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 567 AA; 61456 MW; 260EE57C826032B2 CRC64;
MKLRFLQFDQ KFQQFPAISF TKNIVSLFTF ILFLCTAIPM FAQPGIHDPS STVRNTDGRY
WHFATGNGIY TISSTSTSFG SWQTENTVFP IGTWPSWIAN YVSGFAGNFW APDVVKIGST
YYCYYSCAGD GAPAAIGLAT ATNLSGPWTD KGMIVAGNNA IDPAVLVDGS NMYMVYGNWQ
SGIDLIQLNP STGLRLNSSR WDLVAGNVEA PYIMKNGSYY YVFFQRGLCC NGVNSGYYTQ
VGRSTSITGP YLDQNGISLM NDGGTTFLPN KNGRYIGPGH VGYGESTLSY HFYDGNDGGA
PKLMTTTLSW SNGWPVPGSA SQIIPNGTYK LVNRTGGKYL DNLGATTDGA NVAQWAGGSS
NNQRWVITYS GGYYKLKCVT GGKYLDNIAH TADGSTVGQW TGGTSTNQQW TISAAGPYYK
IINRSNGKCV DNGGVTTDGA VMQFWPSNVS FNQQWTLEFV SSSTAKMSNA ESVVSDEPFA
DENAGLSIVP NPVRGNEFEI IMYNKSETSI TVTLTNMSGQ TVYTKNLGIT KPGEFRYTVN
ASTLSKGTYL VNLSSETGNK TAKIIRN
//
