ID J3EQ34_9LACO Unreviewed; 1173 AA.
AC J3EQ34;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 53.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=A11Y_119167 {ECO:0000313|EMBL:EJN55385.1};
OS Loigolactobacillus coryniformis subsp. coryniformis CECT 5711.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Loigolactobacillus.
OX NCBI_TaxID=1185325 {ECO:0000313|EMBL:EJN55385.1, ECO:0000313|Proteomes:UP000007271};
RN [1] {ECO:0000313|EMBL:EJN55385.1, ECO:0000313|Proteomes:UP000007271}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CECT5711 {ECO:0000313|Proteomes:UP000007271};
RA Rodriguez J.M.;
RT "Complete Genome Sequence of Lactobacillus coryniformis CECT5711.";
RL Submitted (MAY-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJN55385.1}.
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DR EMBL; AKFP01000055; EJN55385.1; -; Genomic_DNA.
DR RefSeq; WP_003679464.1; NZ_AKFP01000055.1.
DR AlphaFoldDB; J3EQ34; -.
DR STRING; 1185325.A11Y_119167; -.
DR GeneID; 65916208; -.
DR PATRIC; fig|1185325.3.peg.2002; -.
DR Proteomes; UP000007271; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11140; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR048635; MFD_D3.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21132; MFD_D3; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000007271}.
FT DOMAIN 635..796
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 821..971
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1173 AA; 132048 MW; A45CD56B0ADAEF10 CRC64;
MDLTELLLQA PKIKDWLGQV TPHSRQLVTG LQDSARSLLL SAILRAKKQP MLVVTNSLFN
ASQLVDDLTN LLPETQVKLF PVDEVMAAQV AVSSPEYRSE RVQALTFLAQ QQPGIVVASL
AGIRQLLPTP EMWRASALTI KPGGELDPLQ LRQQLVGMGY TREQLVGRPG EFAMRGDIID
IYPLTAANPV RIDLFDTEVD SLRYFEASTQ RSIENLSEFT LLPATDFLVT PELLQQAAPT
VKKAYQTELA TLKEASERTA LTDHLTPVIA ALEKGEVVPE LVRYRDLIYA PAANLTDYLP
DDGLVILDDY PRMQESETEL AADAAQWLSE ELANQRMLHS QSVSTELKTL LRKNKHTHLY
LTLFQKGMGN LRLDQILDLQ NRDMQQFFGQ MPLLKAEIDR WQVQQQTVVV MVGDDERRER
VGRILRDFKM QATLAKPDTL QLQQVQIVAA TLQNGFELPD ANLVVITEKE LFNQPIKKRV
RRQTLANAER IKSYNELNPG DYVVHVNHGI GKYVGMQTLE VDGAHQDYIT ILYQDNAKLF
IPVSQLNLVQ KYVASEGKTP RVNKLGGSEW AKTKRKVSSK IEDIADDLIE LYAQREAEKG
YAFSPDNEYQ REFEDAFPYT ETDDQLRSVA EIKHDMERPK PMDRLLVGDV GFGKTEVALR
AIFKAIQDGK QAAFLVPTTI LAQQHFDTMM QRFADFPVQV ELLSRFRTAK QIKASLAAIK
SGQADVVIGT HRILSKDVEF RDLGLLVVDE EQRFGVKAKE RLKTLRSNVD VLTMTATPIP
RTLNMSMVGV RDLSVIETPP ANRYPVQTYV MEQNAGALRS GIERELARGG QVFYLHNRVE
DIERTVANIE ALVPEATVTY AHGQMTEAQL ENTLYEFVHG QYDVIVTTTI IENGVNMPNV
NTLFVEDADH MGLAQLYQLR GRVGRSSRVG YAYFMYRQDK VLTEISEKRL AAIRDFTELG
SGFKIAMRDL SIRGAGNLLG KQQHGFIDSV GYDLYSQMLA DAVAQKQGKA VQAKSDAELD
LGIEAYLPSE YITDQRQKIE LYKRVRELSS PADLEELQAD LFDRFGDYPD PVAHLLAIGL
LKVYADQALV ERIRHVDQQL RVKLSAKGTR QIAPEDVFKA LSKTKLKATV KMDGDKLVIT
LIIQPKMRQP DWLEELTQFL TGLAAIVQQA TPA
//
