ID J3MNF0_ORYBR Unreviewed; 601 AA.
AC J3MNF0;
DT 03-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 03-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
OS Oryza brachyantha (malo sina).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=4533 {ECO:0000313|EnsemblPlants:OB07G29320.1};
RN [1] {ECO:0000313|EnsemblPlants:OB07G29320.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IRGC 101232 {ECO:0000313|EnsemblPlants:OB07G29320.1};
RX PubMed=23481403;
RA Chen J., Huang Q., Gao D., Wang J., Lang Y., Liu T., Li B., Bai Z.,
RA Luis Goicoechea J., Liang C., Chen C., Zhang W., Sun S., Liao Y., Zhang X.,
RA Yang L., Song C., Wang M., Shi J., Liu G., Liu J., Zhou H., Zhou W., Yu Q.,
RA An N., Chen Y., Cai Q., Wang B., Liu B., Min J., Huang Y., Wu H., Li Z.,
RA Zhang Y., Yin Y., Song W., Jiang J., Jackson S.A., Wing R.A., Wang J.,
RA Chen M.;
RT "Whole-genome sequencing of Oryza brachyantha reveals mechanisms underlying
RT Oryza genome evolution.";
RL Nat. Commun. 4:1595-1595(2013).
RN [2] {ECO:0000313|EnsemblPlants:OB07G29320.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (APR-2013) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000256|ARBA:ARBA00000094};
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall
CC {ECO:0000256|ARBA:ARBA00004191}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR AlphaFoldDB; J3MNF0; -.
DR STRING; 4533.J3MNF0; -.
DR EnsemblPlants; OB07G29320.1; OB07G29320.1; OB07G29320.
DR Gramene; OB07G29320.1; OB07G29320.1; OB07G29320.
DR eggNOG; KOG0228; Eukaryota.
DR HOGENOM; CLU_001528_6_0_1; -.
DR OMA; RTWVVFL; -.
DR Proteomes; UP000006038; Chromosome 7.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR PANTHER; PTHR31953:SF61; GLYCO_HYDRO_32N DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Reference proteome {ECO:0000313|Proteomes:UP000006038};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 30..601
FT /note="beta-fructofuranosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003774128"
FT DOMAIN 54..363
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 366..591
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 601 AA; 65964 MW; 10BE7D03BB3C82C5 CRC64;
MAAVRSSWMS VTLLLAALVL APSLAAVEAS HHAFADLQPL EADHVDDTLR TGFHFQPPRH
WINDPNGVMY HNGVYHLFYQ YNPKAAVWGN IVWAHAVSTD LVNWVLLDPA IYPTGPFDEN
GCWSGPAPVL PDGTPVIMYT GIDADGRQVQ NVAYPKDLTD PYLREWVKPD YNPVIAPDAG
VNATAFRDPT TAWQGPDGVW RLVIGTKDNH RGFAALYRSR DFKHWAPARR ALHSGDTGMW
ECPDFYPVTY SVGDGGGTKH VLKVSLDLTR FEYYTFGEYD HASDTYVPDA ALADGNDGLR
YDYGNFYASK TFLDPAKHRR VLWGWANESD STAADVLKGW AGVQAIPRKI WLAPNGKQLL
QWPVAEIESL RGNHVNITDT LVRGGGSYFE VTGLATPAQA DVEASFQVMD VDKAEPFDPA
WRGADAQTTV MDVDKAEPFN PAWRGADAQT VCAARGADAK GGVGPFGLWV LASDELKERT
AVFFSVFKRD TDGGGNKHVV LMCTDPSRSS YAEHLYKPTF AGFVDVDIAE TGKIPLRTLI
DHSVVESFGG HGKTAILSRV YPTMAVGDKA RLFVFNNGES DVKVTSLNAY DMGSAKIRAE
T
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