ID J5GP48_9FIRM Unreviewed; 647 AA.
AC J5GP48;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN Name=pulA {ECO:0000313|EMBL:EJP20148.1};
GN ORFNames=HMPREF1140_1744 {ECO:0000313|EMBL:EJP20148.1};
OS Lachnoanaerobaculum sp. ICM7.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Lachnospiraceae;
OC Lachnoanaerobaculum.
OX NCBI_TaxID=936594 {ECO:0000313|EMBL:EJP20148.1, ECO:0000313|Proteomes:UP000006599};
RN [1] {ECO:0000313|EMBL:EJP20148.1, ECO:0000313|Proteomes:UP000006599}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ICM7 {ECO:0000313|EMBL:EJP20148.1,
RC ECO:0000313|Proteomes:UP000006599};
RA Durkin A.S., McCorrison J., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJP20148.1}.
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DR EMBL; ALJL01000024; EJP20148.1; -; Genomic_DNA.
DR RefSeq; WP_009663481.1; NZ_ALJL01000024.1.
DR AlphaFoldDB; J5GP48; -.
DR PATRIC; fig|936594.3.peg.1658; -.
DR Proteomes; UP000006599; Unassembled WGS sequence.
DR GO; GO:0051060; F:pullulanase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 2.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Glycosidase {ECO:0000313|EMBL:EJP20148.1};
KW Hydrolase {ECO:0000313|EMBL:EJP20148.1}.
FT DOMAIN 178..545
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 647 AA; 74193 MW; 77BAB9054205AE46 CRC64;
MENIIELKKY YSSKEFLENY IYEGNDLGVE CNENGTTFKL WSPGAKSVVL NFYEAGDGCK
AYEQIPMEKE DKGVWSFHSH KELHKVYYDY LIKRDSKEVL TADPYAKASG VNGIRSMAVN
LKKTDPKGWE NDKAPQKDKE RVVYELHVKE FSFAKSGGFP PEYRKKYKAF TCKHTTLNND
GIHPTGLDYL KELGVTHIQI MPMYDYGSVD ETKDYEFNWG YDPVNYNVPE GSYATDAFHG
EVRITEMKEM IQSIHEAGFG VIMDVVYNHT YSLDSWFQRT LPWYFYRVFS DGKVSDGSAC
GNDVASERAM CSKYILESVL YWAREYHIDG FRFDLMGLLD TDLMNNIRKE LDIIYGKGEK
IIYGEPWSAT DTAIEYNKKL ANKYNIALLD ENIGVFCDNT RDAIKGSALR IREAGFVNGG
KDKEDDILAS ISAWCDPKYN KEFKGVKAPS QIVTYVSAHD NQTLWDKLDY TVTEEEAMRL
NKLAAAIYMT CQGTLFFLSG EEFCRTKAGH DNTYNMPISV NMLDWRLAYK NKDLVDYYKG
LIALRKQISG LCDKSENSYK HITDMWKQKQ VVGFSVNSDK DSLWSQVKVI YNASKKDFEV
KSLDGDFEVL CDGNDSMLWK KNISAKAPIK VGKQSVLILG KKRIEEI
//
