ID J6HIM2_9FIRM Unreviewed; 1122 AA.
AC J6HIM2;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 55.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:EJU22488.1};
GN ORFNames=HMPREF1152_1228 {ECO:0000313|EMBL:EJU22488.1};
OS Mogibacterium sp. CM50.
OC Bacteria; Bacillota; Clostridia; Eubacteriales;
OC Eubacteriales Family XIII. Incertae Sedis; Mogibacterium.
OX NCBI_TaxID=936375 {ECO:0000313|EMBL:EJU22488.1, ECO:0000313|Proteomes:UP000003142};
RN [1] {ECO:0000313|EMBL:EJU22488.1, ECO:0000313|Proteomes:UP000003142}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CM50 {ECO:0000313|EMBL:EJU22488.1,
RC ECO:0000313|Proteomes:UP000003142};
RA Durkin A.S., McCorrison J., Torralba M., Gillis M., Methe B., Sutton G.,
RA Nelson K.E.;
RL Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJU22488.1}.
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DR EMBL; ALNL01000017; EJU22488.1; -; Genomic_DNA.
DR RefSeq; WP_009643566.1; NZ_ALNL01000017.1.
DR AlphaFoldDB; J6HIM2; -.
DR STRING; 936375.HMPREF1152_1228; -.
DR PATRIC; fig|936375.3.peg.473; -.
DR eggNOG; COG1197; Bacteria.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000003142; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000003142}.
FT DOMAIN 585..746
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 763..921
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1122 AA; 127193 MW; 18CCD87A60252D33 CRC64;
MKTNITGISG SRIAYYAAKE IVKNNKTLII VSRANVAERL RDDISFFVHD RAIYVMPEED
DLQIIYEAKD SNMLVQRIQA VDSLVSDDKT VVIAPVSAAL RPLQPVERFM ESVIELEMGV
RVEPAELRQK LVSIGYSPAP VVEAPGEFSA RGGIIDIYSP NNSDPVRIEF FDDEIDSLRF
FDPKSQLSFE NIDFVRVCPA AEFLPTAYER RRALSRITDE YDERLSRLKA ESGEGKLSDG
RIDRIEELKG RISEMFENCT NVQIFANYLK YFDVTDERIW DYVIDPADAN MMIYDPNRVA
AEIPEYQEQG DINKFYDKDI IAFTPFPEPI KGIDVFDKVV NVPSRQIASF NGQMELFGRE
MRRLSKEGVE VHIVSADKER HDRIREYLED ARIFGHFRYD LGLLGSGMML DDEKLCYITD
TDIFPNIRKS SKKRLRHRSG KQDFLDLHAG DYVVHEEHGI GRFEGIKTLE ADGEIKDYLK
IHYSGTDVLY IPTEQMDIVQ RYIGSEGKAP KLSSLSGGDW RNTRARARKA IEEIAEDLVK
LYAEREMKGG YAFQHDTVWQ REFEDSFLYQ ETDDQLQAIE EIKADMEKPL PMDRLLCGDV
GYGKTEVAAR AIFKCVAEGK QAVFLAPTTL LANQHYNTLK ERFQDYPFNI EMLSRFVDET
RQKRVLNDIA KGNVDLVIGT HRLLSDDVAY KDLGLLVIDE EQRFGVKHKE KLKMARQTID
VLTLSATPIP RTLNMSLTGI KNISTIEEPP GDRYPVQTFV SAQDDELIRG AIERELARGG
QLYVIYNRVN GINDIAKHIN KLVPEATVAV GHGRMNENTL ENVMLDFVEG DVDIFVTTTI
IENGIDIPNA NTIIILNSDK LGLSQLYQLR GRVGRSNRLA YAYLLYKPER VLTEVATKRL
AAIREFTEFG AGFKLAMRDL ELRGAGNVLG EAQHGNIAGI GYELYVKEID KAVRRLKGEK
ITESREEISI EVDISARIPS EYIKDETLKL QAYKKIAQIT SHEEAEELIA ELIDRYGDLP
DVTIDLVRIA EIKCLAAELG VKNISERGGR FVVEFNEDNN ADAFTLVVAK QKFGEGLIIS
SGSNPYLSLQ KTKEKPAARI LDLMTVMKGA RLQGLAHSEM SN
//
