ID J7HA37_9VIRU Unreviewed; 1727 AA.
AC J7HA37;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE SubName: Full=Replicase {ECO:0000313|EMBL:AFP95341.1};
OS Grapevine virus F.
OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes;
OC Tymovirales; Betaflexiviridae; Trivirinae; Vitivirus.
OX NCBI_TaxID=1221437 {ECO:0000313|EMBL:AFP95341.1, ECO:0000313|Proteomes:UP000201911};
RN [1] {ECO:0000313|EMBL:AFP95341.1, ECO:0000313|Proteomes:UP000201911}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AUD46129 {ECO:0000313|EMBL:AFP95341.1};
RX PubMed=22879616; DOI=10.1128/JVI.01444-12;
RA Al Rwahnih M., Sudarshana M.R., Uyemoto J.K., Rowhani A.;
RT "Complete genome sequence of a novel vitivirus isolated from grapevine.";
RL J. Virol. 86:9545-9545(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
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DR EMBL; JX105428; AFP95341.1; -; Genomic_RNA.
DR RefSeq; YP_006590065.1; NC_018458.1.
DR GeneID; 13466402; -.
DR KEGG; vg:13466402; -.
DR Proteomes; UP000201911; Genome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0008174; F:mRNA methyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR GO; GO:0006396; P:RNA processing; IEA:InterPro.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR CDD; cd23245; Betaflexiviridae_RdRp; 1.
DR Gene3D; 2.60.120.590; Alpha-ketoglutarate-dependent dioxygenase AlkB-like; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR InterPro; IPR027351; (+)RNA_virus_helicase_core_dom.
DR InterPro; IPR027450; AlkB-like.
DR InterPro; IPR037151; AlkB-like_sf.
DR InterPro; IPR002588; Alphavirus-like_MT_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR005123; Oxoglu/Fe-dep_dioxygenase.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR001788; RNA-dep_RNA_pol_alsuvir.
DR InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR Pfam; PF13532; 2OG-FeII_Oxy_2; 1.
DR Pfam; PF00978; RdRP_2; 1.
DR Pfam; PF01443; Viral_helicase1; 1.
DR Pfam; PF01660; Vmethyltransf; 1.
DR SUPFAM; SSF51197; Clavaminate synthase-like; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51743; ALPHAVIRUS_MT; 1.
DR PROSITE; PS51471; FE2OG_OXY; 1.
DR PROSITE; PS51657; PSRV_HELICASE; 1.
DR PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022806};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000201911};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953}.
FT DOMAIN 66..241
FT /note="Alphavirus-like MT"
FT /evidence="ECO:0000259|PROSITE:PS51743"
FT DOMAIN 655..748
FT /note="Fe2OG dioxygenase"
FT /evidence="ECO:0000259|PROSITE:PS51471"
FT DOMAIN 910..1189
FT /note="(+)RNA virus helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51657"
FT DOMAIN 1487..1594
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50507"
SQ SEQUENCE 1727 AA; 197001 MW; 48A0EF1DDB83B255 CRC64;
MSISVSSQRV AAASLYTNGD KTIIDQIKVI KSERLKKIEV EVDGLFDYYV SDFMRDFISK
LGIHTSVHSF VAHPHPVSKM FENYILYRCI SSYIGNSNLF ISCKEAKVKR FKFGRNGLEA
KNTRYNRLIH SKDHFRYEWC TRNKNLEHIP EVREGTKTCD TVVIHDEVQY WSLADLQNFL
GNVEDNTNVL YSVVYPAEIT TGITFSLFPE AYEFEVHDGY FIWYPDGKAE GAYKQPINPW
LLTTSKTEDV RYRTWTITKL QTVGAHHLFS CTPGSTITED SYTYDQFTLI NPYNCFAGRV
HGRQTVRLRA RMVKPVLLYL MALRKPDCGS AVAKIRMLSH DEESCEEAIF VAELAKLISD
LSLYNSMGAF NAWEAVKKVF HRAMGPWWTY LVNRRLFNED SMHEFIANCD SAVITVPRIY
RACVEVRKTL RAPLGHHWHE DEEVFGLLYL SELAKGDRCS EVYSIPAREL QEARVVAPIT
RILRSHELVR EQMLVQEVEL NTVHIAATPG NLIKMFRKCV IRLSAFRFME TRLLTEYNSV
EESLTNGWMT DERAMEILFG VVEAPILQEK VSEVEMPTAD HTVGPATCSK ITDATKGKVE
VFLKQVKYLD KLKGRVCAFY SKYSTGYTYT GGSHISQGMP ESLVELRRSM GLGEEYDHCL
VQKYEKDGAI PFHADDEKCY LPGSCVVTLN MHGTAEFMLK ENGKEEVHRV TLEDGDVLVM
PEGVQENYKH SVLVKSEGRI SLTFRNKTVD YMNSDAVSDY EEDAPDVDDG LRSIQANRAN
LCTLQCIADH IKLDLPILVA RLNATNPEYI SELSKGGPTL PTFINLCAKM DIPLQISGVH
GGLQTRGNFK KLCVFMGEDH VERVEETVGR NNAYDAFSLH PNSGRGTFYP NREYAEKLSE
SMFEGHTGIL LNKFKRGKSV IKDVRKEIKF VSFFGFAGSG KSYYPQTVLQ HTFGIRALVI
SPRKALAADW SSKIGSRAKV VTFEKAVVEE EVFSCIVVDE LGLYPPGYLD LICLLHEPST
LVLLGDPLQG TYYNKDDRIK LATVSHNVFA RLEGSLPYLM YSHRLSKSNR TFDIDTHGNL
ESAGSQRVKK LDTKMPTIYA ARATKESAEG TAYTVSETQG LSFKDVQVLI DKDWALKEDG
DVIVAFTRAR GTTKYMCSDS DMDMLKARAK SEVLRSILRA GKVGRDTILN SLRKEMPNAE
PVFTEARLAN TEEYEEKLMG DPYLKGMLRL LQEEEIEEEE CEEPMAPEPV KTHLFLSEGV
NEMAPFMFVN QSGQRVKLGD RFNREQKTTA GLTAQIDEMG YAGQNENPCT HKALYLHHKM
DXAATFMLSV RKRLRFRDPE KNRARYEKHK GIGRQMFDVL KKTYHWTSID SLPPLEKAEA
DFMKKRLNKS AALLERHNIR SDPDWPSNII KIFLKQQVCT KLEKRGVDAK AGQTIACFSH
AVLCKFGPIM RQTERKLREL LPPNVMIYSQ KNYSDLDVWA KTYVDTMVGT DSDYEAFDRS
QDEKILDLEV EVLKFFLWPE ELIREYVELK LQMSCSMGNL AIMRFSGEFG TFFFNTMCNM
VFTCMRYNVT DKTRLCFAGD DMYAPGVLEV RTDYEHILDE LSLKAKVQVG DRPLFCGWRM
SPYGIVKEPN LVLDRWKIAK GNGSLHDCMV NYAIEASYGY RLSEYLYDIN IDIDAQQELT
RQIVLVKDKL PPKVSSIFSD DANESWSDGD DEFLIKLSEP QLRMGLF
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