ID J7L8C7_NOCAA Unreviewed; 819 AA.
AC J7L8C7;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 27-MAR-2024, entry version 49.
DE SubName: Full=Penicillin amidase family protein {ECO:0000313|EMBL:AFR07680.1};
GN OrderedLocusNames=B005_1427 {ECO:0000313|EMBL:AFR07680.1};
OS Nocardiopsis alba (strain ATCC BAA-2165 / BE74).
OC Bacteria; Actinomycetota; Actinomycetes; Streptosporangiales;
OC Nocardiopsaceae; Nocardiopsis.
OX NCBI_TaxID=1205910 {ECO:0000313|EMBL:AFR07680.1, ECO:0000313|Proteomes:UP000003779};
RN [1] {ECO:0000313|EMBL:AFR07680.1, ECO:0000313|Proteomes:UP000003779}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2165 / BE74 {ECO:0000313|Proteomes:UP000003779};
RX PubMed=23105086; DOI=10.1128/JB.01522-12;
RA Qiao J., Chen L., Li Y., Wang J., Zhang W., Chen S.;
RT "Whole-Genome Sequence of Nocardiopsis alba Strain ATCC BAA-2165,
RT Associated with Honeybees.";
RL J. Bacteriol. 194:6358-6359(2012).
RN [2] {ECO:0000313|Proteomes:UP000003779}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-2165 / BE74 {ECO:0000313|Proteomes:UP000003779};
RA Qiao J., Chen L., Li Y., Wang J., Zhang W., Chen S.;
RT "Whole-genome sequence of Nocardiopsis alba strain ATCC BAA-2165 associated
RT with honeybees.";
RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|PIRSR:PIRSR001227-2};
CC Note=Binds 1 Ca(2+) ion per dimer. {ECO:0000256|PIRSR:PIRSR001227-2};
CC -!- SIMILARITY: Belongs to the peptidase S45 family.
CC {ECO:0000256|ARBA:ARBA00006586}.
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DR EMBL; CP003788; AFR07680.1; -; Genomic_DNA.
DR RefSeq; WP_014910141.1; NC_018524.1.
DR AlphaFoldDB; J7L8C7; -.
DR STRING; 1205910.B005_1427; -.
DR MEROPS; S45.003; -.
DR KEGG; nal:B005_1427; -.
DR PATRIC; fig|1205910.3.peg.1349; -.
DR eggNOG; COG2366; Bacteria.
DR HOGENOM; CLU_011790_0_1_11; -.
DR OrthoDB; 9759796at2; -.
DR Proteomes; UP000003779; Chromosome.
DR GO; GO:0016811; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0017000; P:antibiotic biosynthetic process; IEA:InterPro.
DR CDD; cd03747; Ntn_PGA_like; 1.
DR Gene3D; 1.10.1400.10; -; 1.
DR Gene3D; 2.30.120.10; -; 1.
DR Gene3D; 3.60.20.10; Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1; 1.
DR Gene3D; 1.10.439.10; Penicillin Amidohydrolase, domain 1; 1.
DR InterPro; IPR029055; Ntn_hydrolases_N.
DR InterPro; IPR014395; Pen/GL7ACA/AHL_acylase.
DR InterPro; IPR043147; Penicillin_amidase_A-knob.
DR InterPro; IPR023343; Penicillin_amidase_dom1.
DR InterPro; IPR043146; Penicillin_amidase_N_B-knob.
DR InterPro; IPR002692; S45.
DR PANTHER; PTHR34218:SF4; ACYL-HOMOSERINE LACTONE ACYLASE QUIP; 1.
DR PANTHER; PTHR34218; PEPTIDASE S45 PENICILLIN AMIDASE; 1.
DR Pfam; PF01804; Penicil_amidase; 1.
DR PIRSF; PIRSF001227; Pen_acylase; 1.
DR SUPFAM; SSF56235; N-terminal nucleophile aminohydrolases (Ntn hydrolases); 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|PIRSR:PIRSR001227-2};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001227-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000003779};
KW Zymogen {ECO:0000256|ARBA:ARBA00023145}.
FT REGION 207..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 265
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-1"
FT BINDING 181
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 343
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
FT BINDING 346
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR001227-2"
SQ SEQUENCE 819 AA; 89505 MW; D6A6AAFCC68BE034 CRC64;
MLALIASLLG VWAVRRSFPT TDGELSLPAL GSPVTVLRDE YGVANIYADD AEDLFVAQGF
THAQDRFWEM DFRRHVTAGR TAELFGEAQV ETDVYLRTMG WRRVAEQEYD LLDEDSRSYL
DAYAAGVNAW LDQNDGLSAS LEYGLLGITA DDYDIEPWTP TDSLAWLKAM AWDLGGNLQE
ETDRALLLAN GLDRDLIEEL YPAYPTERHV PITDTDEHDG AAPDADLASA PELPDEALDA
LANLSGVQDT IPDLIGPSGH PDLGSNSWVV SGEHTDTGSP LLTNDPHLGA SMPSTWHQIG
LHCTEVSEAC PFDVSGFGFS GLPGVIIGQN DSIAWGFTNL NPDVMDLYLE RIEGDEYVVD
GEKRPLEIIE DTIEVAGGED VDITIRATHH GPIMSDTGLG ANLATIGENP SEGDDAPYAV
SLGWTALQPG ATADAIFAMN RARDWDEFRA GASRFEVPAQ NLVYADEEGN IGYQAPGRVP
VRGEGDGRWP APGWDSDYDW DEEYLPFDEL PSVLNPESGF IVTANQSVVD EDYEHFLTTD
WDYGYRSQRI NDLLAERIAA GPVTVQDMED VMMDSRHEAA VDITPHLLDV EVDGVTAKAQ
EELADWDHVN DTDSAGAAFY NATWRHLLPL LFDELGDVDM NTGSRGMYVV GLLLEDPESS
WWEGEKADGR EEVLATAMDE AAEELTDLLG DTPADWRWGD LHTLTATHES FGSSGIAPVE
WLFNRGPVES SGGSSIVNAT GWNATEGYGI TTVPSMRMVA NLADRDASTW IHLTGNSGHA
FHPNYDDQLE LWAEGSSLPW AVTEEAVREA STDELTLTP
//
