ID J7RVT6_KAZNA Unreviewed; 581 AA.
AC J7RVT6;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 49.
DE RecName: Full=amidase {ECO:0000256|ARBA:ARBA00012922};
DE EC=3.5.1.4 {ECO:0000256|ARBA:ARBA00012922};
GN Name=KNAG0B06280 {ECO:0000313|EMBL:CCK69057.1};
GN OrderedLocusNames=KNAG_0B06280 {ECO:0000313|EMBL:CCK69057.1};
OS Kazachstania naganishii (strain ATCC MYA-139 / BCRC 22969 / CBS 8797 / KCTC
OS 17520 / NBRC 10181 / NCYC 3082 / Yp74L-3) (Yeast) (Saccharomyces
OS naganishii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kazachstania.
OX NCBI_TaxID=1071383 {ECO:0000313|EMBL:CCK69057.1, ECO:0000313|Proteomes:UP000006310};
RN [1] {ECO:0000313|EMBL:CCK69057.1, ECO:0000313|Proteomes:UP000006310}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-139 / BCRC 22969 / CBS 8797 / CCRC 22969 / KCTC 17520
RC / NBRC 10181 / NCYC 3082 {ECO:0000313|Proteomes:UP000006310};
RX PubMed=22123960; DOI=10.1073/pnas.1112808108;
RA Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P.,
RA Wolfe K.H.;
RT "Evolutionary erosion of yeast sex chromosomes by mating-type switching
RT accidents.";
RL Proc. Natl. Acad. Sci. U.S.A. 108:20024-20029(2011).
RN [2] {ECO:0000313|Proteomes:UP000006310}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-139 / BCRC 22969 / CBS 8797 / CCRC 22969 / KCTC 17520
RC / NBRC 10181 / NCYC 3082 {ECO:0000313|Proteomes:UP000006310};
RA Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P.,
RA Wolfe K.H.;
RT "Genome sequence of Kazachstania naganishii.";
RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a monocarboxylic acid amide + H2O = a monocarboxylate +
CC NH4(+); Xref=Rhea:RHEA:12020, ChEBI:CHEBI:15377, ChEBI:CHEBI:28938,
CC ChEBI:CHEBI:35757, ChEBI:CHEBI:83628; EC=3.5.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001311};
CC -!- SIMILARITY: Belongs to the amidase family.
CC {ECO:0000256|ARBA:ARBA00009199}.
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DR EMBL; HE978315; CCK69057.1; -; Genomic_DNA.
DR AlphaFoldDB; J7RVT6; -.
DR STRING; 1071383.J7RVT6; -.
DR eggNOG; KOG1212; Eukaryota.
DR HOGENOM; CLU_009600_9_2_1; -.
DR OMA; RMTEFAF; -.
DR OrthoDB; 731186at2759; -.
DR Proteomes; UP000006310; Chromosome 2.
DR GO; GO:0004040; F:amidase activity; IEA:UniProtKB-EC.
DR GO; GO:0043864; F:indoleacetamide hydrolase activity; IEA:UniProtKB-EC.
DR Gene3D; 3.90.1300.10; Amidase signature (AS) domain; 1.
DR InterPro; IPR020556; Amidase_CS.
DR InterPro; IPR023631; Amidase_dom.
DR InterPro; IPR036928; AS_sf.
DR PANTHER; PTHR46072:SF4; AMIDASE C550.07-RELATED; 1.
DR PANTHER; PTHR46072; AMIDASE-RELATED-RELATED; 1.
DR Pfam; PF01425; Amidase; 1.
DR PIRSF; PIRSF001221; Amidase_fungi; 1.
DR SUPFAM; SSF75304; Amidase signature (AS) enzymes; 1.
DR PROSITE; PS00571; AMIDASES; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000006310}.
FT DOMAIN 112..565
FT /note="Amidase"
FT /evidence="ECO:0000259|Pfam:PF01425"
FT ACT_SITE 168
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT ACT_SITE 243
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT ACT_SITE 267
FT /note="Acyl-ester intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001221-1"
FT BINDING 217
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT BINDING 243
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
FT BINDING 264..267
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001221-2"
SQ SEQUENCE 581 AA; 64972 MW; 1EABCBB9310C59FE CRC64;
MATKIIERLY NTDPELTYKP DLVKYETVHL PKITSYREEL ASKFPQETVL KPGLLADKGI
EIPLDFESSV FKDGPKLAKA SGFFTDRELE ILELKAIEII ERIAAGEQTA LETITAFIKQ
AAIAQQVTNC AMDFFPEEAF ERARELDKYF AETGKTVGPM HGLPVSVKEH YAFKGKVTSC
GFVSKLNDVE EIDAYITDLF KKTGAVYYIR TTQPQTIMHL DSFNNIIGLC RNPYNTALSP
GGSSSGEAAL VGMRGSPLGC GSDIGGSIRC PAAFCNIWGL KPTARRLSCL GSRSQFTKHS
NEMILPTFGP MANSADDLEL FMKVCSDSKP WLYDNYVLRM PWIKDVKFAI GDLKIAIVLD
DGVVKPSPPI LRALLLAKKA LESAGVGKII EWESFKTVEA LEICYNAYTK DGNFNARAVL
GASGEPLAPL TEHYMRFGTG DKKLSNLEIM ECENRRDVLR QEFQLKMNER DIDFILTPAY
FAPAGIPHKI KYWGYTAVYN ILDLPGVSFP TGIKVNKSID KKDVNYQPRN DMEEYEYPMY
DEEVYDGMPI GLMLQGRRYH DEETLAAAKL IQEVISKASR T
//
