ID   J7S4I1_NAUDC            Unreviewed;       846 AA.
AC   J7S4I1;
DT   31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT   31-OCT-2012, sequence version 1.
DT   24-JAN-2024, entry version 50.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   Name=NDAI0G04830 {ECO:0000313|EMBL:CCK73466.1};
GN   OrderedLocusNames=NDAI_0G04830 {ECO:0000313|EMBL:CCK73466.1};
OS   Naumovozyma dairenensis (strain ATCC 10597 / BCRC 20456 / CBS 421 / NBRC
OS   0211 / NRRL Y-12639) (Saccharomyces dairenensis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Naumovozyma.
OX   NCBI_TaxID=1071378 {ECO:0000313|EMBL:CCK73466.1, ECO:0000313|Proteomes:UP000000689};
RN   [1] {ECO:0000313|EMBL:CCK73466.1, ECO:0000313|Proteomes:UP000000689}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 10597 / BCRC 20456 / CBS 421 / NBRC 0211 / NRRL Y-12639
RC   {ECO:0000313|Proteomes:UP000000689};
RX   PubMed=22123960; DOI=10.1073/pnas.1112808108;
RA   Gordon J.L., Armisen D., Proux-Wera E., OhEigeartaigh S.S., Byrne K.P.,
RA   Wolfe K.H.;
RT   "Evolutionary erosion of yeast sex chromosomes by mating-type switching
RT   accidents.";
RL   Proc. Natl. Acad. Sci. U.S.A. 108:20024-20029(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR   EMBL; HE580273; CCK73466.1; -; Genomic_DNA.
DR   RefSeq; XP_003980142.1; XM_003980093.1.
DR   AlphaFoldDB; J7S4I1; -.
DR   STRING; 1071378.J7S4I1; -.
DR   GeneID; 13926944; -.
DR   KEGG; ndi:NDAI_0G04830; -.
DR   eggNOG; KOG0941; Eukaryota.
DR   HOGENOM; CLU_002173_5_1_1; -.
DR   OMA; YGNDWHI; -.
DR   OrthoDB; 5471864at2759; -.
DR   Proteomes; UP000000689; Chromosome 7.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   CDD; cd00078; HECTc; 1.
DR   Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR   PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000000689};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   DOMAIN          494..846
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   ACT_SITE        814
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   846 AA;  98683 MW;  018DE22CA96DE10E CRC64;
     MGIFSRKNST KRDTAKAKEN ARIEIISRKA SLKRPSVEGK TVSRVGSLKQ NAEKRQCPCC
     NSWIQVPPKI PKFRCSICQV TTYLKMDSSQ QSSTTSVKEN IVCNKFVLEK VINGCEKEVK
     ERKLTSREDF AKVYDSVTIY LTNCFQDATI LNQSFCPENN LDTIDYLSLS EFYEIVMNLP
     TRRPFYRLLC VSNELLRRSN IAMTSFKWIL IILNNPIVRQ ALTGRIPKKF ESVKIRTIAY
     DLGKKCIGFL SNMPYKENSD KLIRYLKSLS VESTLQYIEL LNIYLTFQLH RIVRHETKTA
     LEKYRPFYPG IGNGNTEAWI HDKYALGTNN DKTVVLRDFR FKPFQYEDDW HIKTVTELMR
     KFYQVNVERC NQQGFSNSKK LNVSEFYNTM LDVIDYRKDF DIWRGYTGND QLSKFINQQS
     WNTSSFTFCK YPFLLSLGLK ISIMEYEFKR IMEYEAENAF LTSLDKGKMV NVYFKIRVRR
     NRITNDSLQC IQQHQGDLLK SLRIEFIDEP GIDAGGLRKE WFLLLTKSLF NPMNGLFVSV
     EESNLSWIAI HDIQTLNKSS TFKNELFFLF GVVVALAIFN STILDLQFPR TFYKKLCNEP
     LTFDDYREIY PVTAQNLMKM LQYENDDFEE VFGLTFETTY KDPLKYALDN EKSKGDGMVS
     VELCKNGSKM KVTQANKQKF INLWVNFYLN ESIINQISQF RSGFDRVFAR TKSISLLNSE
     ELERLLCGDE TQQSYDFKML RSVTKYSGGF SDDSRVVAWF WEIVEGWNYK LQRKLLSFIT
     GSDRIPATGI STLNFKISRL GSKDKNNLPL SHTCFNELCL WEYSSRGKLE KKLLYAITES
     EGFGFR
//