ID J8THC2_SACK1 Unreviewed; 533 AA.
AC J8THC2;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
DE AltName: Full=Saccharase {ECO:0000256|ARBA:ARBA00030925};
GN Name=YIL162W {ECO:0000313|EMBL:EJT44543.1};
GN ORFNames=SKUD_196906 {ECO:0000313|EMBL:EJT44543.1};
OS Saccharomyces kudriavzevii (strain ATCC MYA-4449 / AS 2.2408 / CBS 8840 /
OS NBRC 1802 / NCYC 2889) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=226230 {ECO:0000313|EMBL:EJT44543.1, ECO:0000313|Proteomes:UP000002753};
RN [1] {ECO:0000313|EMBL:EJT44543.1, ECO:0000313|Proteomes:UP000002753}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC MYA-4449 / AS 2.2408 / CBS 8840 / NBRC 1802 / NCYC 2889
RC {ECO:0000313|Proteomes:UP000002753};
RX PubMed=12775844; DOI=10.1126/science.1084337;
RA Cliften P.F., Sudarsanam P., Desikan A., Fulton L., Fulton B., Majors J.,
RA Waterston R., Cohen B.A., Johnston M.;
RT "Finding functional features in Saccharomyces genomes by phylogenetic
RT footprinting.";
RL Science 301:71-76(2003).
RN [2] {ECO:0000313|Proteomes:UP000002753}
RP GENOME REANNOTATION.
RC STRAIN=ATCC MYA-4449 / AS 2.2408 / CBS 8840 / NBRC 1802 / NCYC 2889
RC {ECO:0000313|Proteomes:UP000002753};
RX PubMed=22384314; DOI=10.1534/g3.111.000273;
RA Scannell D.R., Zill O.A., Rokas A., Payen C., Dunham M.J., Eisen M.B.,
RA Rine J., Johnston M., Hittinger C.T.;
RT "The awesome power of yeast evolutionary genetics: New genome sequences and
RT strain resources for the Saccharomyces sensu stricto genus.";
RL G3 (Bethesda) 1:11-25(2011).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJT44543.1}.
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DR EMBL; AACI03000339; EJT44543.1; -; Genomic_DNA.
DR AlphaFoldDB; J8THC2; -.
DR STRING; 226230.J8THC2; -.
DR HOGENOM; CLU_001528_3_3_1; -.
DR Proteomes; UP000002753; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18622; GH32_Inu-like; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR018053; Glyco_hydro_32_AS.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42800; EXOINULINASE INUD (AFU_ORTHOLOGUE AFUA_5G00480); 1.
DR PANTHER; PTHR42800:SF2; INVERTASE 2; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR PROSITE; PS00609; GLYCOSYL_HYDROL_F32; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Reference proteome {ECO:0000313|Proteomes:UP000002753};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..533
FT /note="beta-fructofuranosidase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5003816624"
FT DOMAIN 33..337
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 371..507
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 533 AA; 60742 MW; 4799160C2011CF7A CRC64;
MMLLKAFIFL LASFAAKVSA LMTNETSDRP LVHFTPNKGW MNDPNGLWYD EKDAKWHLYF
QYNPNDTVWG TPLFWGHASS DDLTHWQDEP VAIAPKRNDS GAYSGSMVVD YNNTSGFFND
TIDPRQRCVA IWTYNTPESE EQYISYSLDG GYTFTEYQNN PVLAANSTQF RDPKVFWYEP
SQKWIMTAAK SQDYKIEIFS SDNLKSWKLE SAFANEGFLG YQYECPGLIQ VPTEQDPSKS
HWVMFISINP GAPAGGSFNQ YFVGSFNGTH FETFDNQSRV VDFGKDYYAL QTFFNTDPTY
GSALGIAWAS NWEYSAFVPT NPWRSSMSLV RKFSLNTEYQ ANPETRLTNL KAEPILNVSN
AGPWSSFATN TTLTKANSYS IDLSNSTGTL EFELIYAVNT TQTVYKSVFS DLSLWFKGLE
DSEEYLRMGY EVSASSFFLD RGNSKVEFVK ENPYFTNRMS VNNQPFKIES DLSYYKVYGL
LDQNILELYF NDGDAVATNT YFMTTGNALG SVNMTTGVDN LFHIDKFQVR EVK
//
