ID J9HUA9_9SPIT Unreviewed; 1374 AA.
AC J9HUA9;
DT 31-OCT-2012, integrated into UniProtKB/TrEMBL.
DT 31-OCT-2012, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE RecName: Full=Vacuolar protein sorting-associated protein 53 homolog {ECO:0008006|Google:ProtNLM};
GN ORFNames=OXYTRI_09386 {ECO:0000313|EMBL:EJY69872.1};
OS Oxytricha trifallax.
OC Eukaryota; Sar; Alveolata; Ciliophora; Intramacronucleata; Spirotrichea;
OC Stichotrichia; Sporadotrichida; Oxytrichidae; Oxytrichinae; Oxytricha.
OX NCBI_TaxID=1172189 {ECO:0000313|EMBL:EJY69872.1};
RN [1] {ECO:0000313|EMBL:EJY69872.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JRB310 {ECO:0000313|EMBL:EJY69872.1};
RX PubMed=23382650; DOI=10.1371/journal.pbio.1001473;
RA Swart E.C., Bracht J.R., Magrini V., Minx P., Chen X., Zhou Y.,
RA Khurana J.S., Goldman A.D., Nowacki M., Schotanus K., Jung S., Fulton R.S.,
RA Ly A., McGrath S., Haub K., Wiggins J.L., Storton D., Matese J.C.,
RA Parsons L., Chang W.J., Bowen M.S., Stover N.A., Jones T.A., Eddy S.R.,
RA Herrick G.A., Doak T.G., Wilson R.K., Mardis E.R., Landweber L.F.;
RT "The Oxytricha trifallax Macronuclear Genome: A Complex Eukaryotic Genome
RT with 16,000 Tiny Chromosomes.";
RL PLoS Biol. 11:E1001473-E1001473(2013).
CC -!- SUBCELLULAR LOCATION: Endosome membrane
CC {ECO:0000256|ARBA:ARBA00004481}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004481}. Golgi apparatus, trans-Golgi network
CC membrane {ECO:0000256|ARBA:ARBA00004150}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004150}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}. Membrane
CC {ECO:0000256|ARBA:ARBA00004170}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004170}.
CC -!- SIMILARITY: Belongs to the VPS53 family.
CC {ECO:0000256|ARBA:ARBA00008628}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EJY69872.1}.
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DR EMBL; AMCR01017507; EJY69872.1; -; Genomic_DNA.
DR EnsemblProtists; EJY69872; EJY69872; OXYTRI_09386.
DR OrthoDB; 5266663at2759; -.
DR GO; GO:0005829; C:cytosol; IEA:GOC.
DR GO; GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000938; C:GARP complex; IEA:InterPro.
DR GO; GO:0016409; F:palmitoyltransferase activity; IEA:InterPro.
DR GO; GO:0042147; P:retrograde transport, endosome to Golgi; IEA:InterPro.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR InterPro; IPR039766; Vps53.
DR InterPro; IPR031745; Vps53_C.
DR InterPro; IPR007234; Vps53_N.
DR PANTHER; PTHR12820:SF0; VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN 53 HOMOLOG; 1.
DR PANTHER; PTHR12820; VACUOLAR SORTING PROTEIN 53; 1.
DR Pfam; PF01529; DHHC; 1.
DR Pfam; PF16854; VPS53_C; 1.
DR Pfam; PF04100; Vps53_N; 1.
DR PROSITE; PS50216; DHHC; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Endosome {ECO:0000256|ARBA:ARBA00022753};
KW Golgi apparatus {ECO:0000256|ARBA:ARBA00023034};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 477..495
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 516..537
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 431..556
FT /note="Palmitoyltransferase DHHC"
FT /evidence="ECO:0000259|Pfam:PF01529"
FT DOMAIN 621..1044
FT /note="Vps53 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04100"
FT DOMAIN 1268..1362
FT /note="Vps53 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16854"
FT REGION 338..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 638..697
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1374 AA; 158996 MW; EB62E4B5F2A09DD2 CRC64;
MINFNVSKSF DLSGQFQQQY ENLNQKKLNK LQNPIKTTLH DSPLFKKHKK NLSVALNQSL
DIHSRNHFKL NQLDNQTLTG LNSSYGKRSF FDSINYSKVY QRIQGPQLQL QQVIDTNNDT
LRSLVQKEKF KKDYVQNKAF VTLKPKLIRV TSLGFVIKLG NTKLRFSRIN IAFQSEFWIG
QRDLSKSPNH PQVIKQSRYQ GANSQEEVDQ QQLVSRCVIQ SQERKKQVYP ETSRSILKTE
KVVNKRDLKN RTIRFQMNSN EGLKIGVWEE LLAGSIWGYI HLLVFSFNVF MIIASHLKCM
TTEPGVLPRD YEELDSNKLP QELSLALNQI QNSLLLEEEE SQQNQSLETT QNTSLDSQAS
TNDSGNNDQY NIDVKGLKFE QFKKGQDKEA MKSILKSVNN KSKVQTDSED QPLNRVVVNA
KNVRFLDKIL SKKCKKCNSI KPPKSHHCST CKRCVARMDH HCPWVNNCVG FYTQKHFILF
LFYVFNGSGH ALYLLCKNSI YCLDKNCAMF NSYSNIALTA IAIFLALLFC LFVSIMFCDQ
ISCIINNTST IDKLQKHEET KKDRKSKKSS WQQIDLVFSG GKGFSIWWFI PVDIPQQVTV
EESALEEEFG KLEDDPLLSS KFDINEYINS HFGDEQSLNQ IEQKIVNFDD QIQDLDLKIK
ECIREQAYAS EGARDELKQI NEESAFLIDR IQSVKQKADV SEEMVKSICS EIRSLDFAKK
NITFTITSLK RMIMLITGIE QLRGFCINKQ YREASNLIEA TDELCNYFKE YKDIQQIKDL
RKERDHLCNQ LRLQIFEDFN KIDKGSLQDL LYEACFVIDA LGEQAVNEVR NWFCNFILEP
YKKLFAPGQP EASFENTKRR FAWLRRTLKE FQDKYESIFP SEWNLKPMIA YEFCRNTKLH
IDDILSTQHH SIDVSIIIDI LQSTIDFEHD LHKRFASHQL QNNNLGSDNL DDMSSHQVKV
DQFGNVKIEG GTAKEIKEKY KDLAKKSDDQ EQHNKVYEKI RGKTKMSAHI YRFKGCISEC
FEPYLKSYSE SEERKIIDAV ETVMGLGDKL ETIDYTVYGS SLHLFKNIKG SLKRCISFST
SKALFDLHTS FKNAFRHFSK LLRRNIPKLG TETEKISITD EQEIKCCYII NTCEYCLETI
PGLHNSIVDK IDEQYKEKID LNIAQDTFRE LINQCIQGLI SSLEAKADVQ YSSVMQKINW
ATFSGVQDTS YYFRTICQTI QKRVMQIKGA LNPVYFTYLL NKIAAAVPAQ YLIQVYKIKK
VNDESSQQFL LDLVEMKTTL SNLPLLQADN SQNSGQNSGQ QINQVVKIPE SYLNFVNKNI
LKLEGRFKVL GYPIEQIKDA YQTLVKDKTQ EDLEQILTIR GVKKSDFGLF KLQL
//
