ID K0KKW6_WICCF Unreviewed; 571 AA.
AC K0KKW6;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE SubName: Full=Sterol O-acyltransferase 2 {ECO:0000313|EMBL:CCH42777.1};
DE EC=2.3.1.26 {ECO:0000313|EMBL:CCH42777.1};
GN Name=ARE2 {ECO:0000313|EMBL:CCH42777.1};
GN ORFNames=BN7_2321 {ECO:0000313|EMBL:CCH42777.1};
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466 {ECO:0000313|EMBL:CCH42777.1, ECO:0000313|Proteomes:UP000009328};
RN [1] {ECO:0000313|EMBL:CCH42777.1, ECO:0000313|Proteomes:UP000009328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10 {ECO:0000313|Proteomes:UP000009328};
RX PubMed=23193139; DOI=10.1128/EC.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Ruckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puhler A., Schaffer S.,
RA Tauch A., Kohler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
CC -!- FUNCTION: Sterol O-acyltransferase that catalyzes the formation of
CC stery esters. {ECO:0000256|ARBA:ARBA00023568}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004477}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004477}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the membrane-bound acyltransferase family.
CC Sterol o-acyltransferase subfamily. {ECO:0000256|ARBA:ARBA00009010}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH42777.1}.
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DR EMBL; CAIF01000051; CCH42777.1; -; Genomic_DNA.
DR AlphaFoldDB; K0KKW6; -.
DR STRING; 1206466.K0KKW6; -.
DR eggNOG; KOG0380; Eukaryota.
DR HOGENOM; CLU_018190_2_1_1; -.
DR InParanoid; K0KKW6; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0034736; F:cholesterol O-acyltransferase activity; IEA:UniProtKB-EC.
DR InterPro; IPR004299; MBOAT_fam.
DR InterPro; IPR014371; Oat_ACAT_DAG_ARE.
DR PANTHER; PTHR10408; STEROL O-ACYLTRANSFERASE; 1.
DR PANTHER; PTHR10408:SF9; STEROL O-ACYLTRANSFERASE 1-RELATED; 1.
DR Pfam; PF03062; MBOAT; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:CCH42777.1};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000009328};
KW Transferase {ECO:0000313|EMBL:CCH42777.1};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 158..178
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 198..219
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 231..248
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 371..389
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 409..433
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 551..569
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REGION 1..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..74
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 571 AA; 66504 MW; D4FDE679D9146D4D CRC64;
MMEGPKRKYT DDSQLKELES MNRNRQSLAF DEDYESSKMA LNETPTPLES GSGSGSGSGS
GSSTTNMGTP EPLNQISTNI EAKLGKVEAG EIKTHQPKLK LVRKATDQDL YELKTKVIVD
NKRFKDGLPK SKLSDVKFSN VDSILDHSAT KTNRFAGFYI LFWFGVSVTV IDSFVNYILT
NGFHSQIVAL LLTDLFDIAF TDLVMYLSIY FVFILHSFVS KGFISWTKTG WIILSIYELV
YVYTFMSIAE HRNFPWIGKI FLFLHQLVLL MKMHSYGFYN GYLWRIKEEL EFSKAFQKKN
EDIDDDHIKK LIEKSIEFCE FELQSQSINE SFPQNINLKN FFNYTMFPTL VYQIDFPRTN
KIRWKYVGEK LAAIFGVITL MIVIAQNYMY PLAIRAIQLR ELSLFDRILQ YPLLLLNIMP
PFLLIYILVW YLIWDAILNC IAELSKFGDR DFYGDWWNCI SWDQFAREWN IPVHKFLLRH
VYHSSISAFK LSKTSATIFT FLLSSIVHEM AMYVLFHKMR GYLLLLQMFQ LPLVAISKSK
YLKERRTLGN VMFWFGIVSG PSLMCTLYLT F
//
