ID K0KN23_WICCF Unreviewed; 571 AA.
AC K0KN23;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=Carboxylic ester hydrolase {ECO:0000256|RuleBase:RU361235};
DE EC=3.1.1.- {ECO:0000256|RuleBase:RU361235};
GN ORFNames=BN7_2298 {ECO:0000313|EMBL:CCH42754.1};
OS Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM
OS 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) (Yeast) (Pichia ciferrii).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Phaffomycetaceae; Wickerhamomyces.
OX NCBI_TaxID=1206466 {ECO:0000313|EMBL:CCH42754.1, ECO:0000313|Proteomes:UP000009328};
RN [1] {ECO:0000313|EMBL:CCH42754.1, ECO:0000313|Proteomes:UP000009328}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL
RC Y-1031 F-60-10 {ECO:0000313|Proteomes:UP000009328};
RX PubMed=23193139; DOI=10.1128/EC.00258-12;
RA Schneider J., Andrea H., Blom J., Jaenicke S., Ruckert C., Schorsch C.,
RA Szczepanowski R., Farwick M., Goesmann A., Puhler A., Schaffer S.,
RA Tauch A., Kohler T., Brinkrolf K.;
RT "Draft genome sequence of Wickerhamomyces ciferrii NRRL Y-1031 F-60-10.";
RL Eukaryot. Cell 11:1582-1583(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a triacylglycerol + H2O = a diacylglycerol + a fatty acid +
CC H(+); Xref=Rhea:RHEA:12044, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:17855, ChEBI:CHEBI:18035, ChEBI:CHEBI:28868; EC=3.1.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00001024};
CC -!- SIMILARITY: Belongs to the type-B carboxylesterase/lipase family.
CC {ECO:0000256|ARBA:ARBA00005964, ECO:0000256|RuleBase:RU361235}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCH42754.1}.
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DR EMBL; CAIF01000051; CCH42754.1; -; Genomic_DNA.
DR AlphaFoldDB; K0KN23; -.
DR STRING; 1206466.K0KN23; -.
DR ESTHER; wiccf-k0kn23; Fungal_carboxylesterase_lipase.
DR eggNOG; KOG4389; Eukaryota.
DR HOGENOM; CLU_006586_10_6_1; -.
DR InParanoid; K0KN23; -.
DR Proteomes; UP000009328; Unassembled WGS sequence.
DR GO; GO:0004806; F:triglyceride lipase activity; IEA:UniProtKB-EC.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002018; CarbesteraseB.
DR InterPro; IPR019826; Carboxylesterase_B_AS.
DR InterPro; IPR019819; Carboxylesterase_B_CS.
DR PANTHER; PTHR11559; CARBOXYLESTERASE; 1.
DR PANTHER; PTHR11559:SF370; CARBOXYLIC ESTER HYDROLASE-RELATED; 1.
DR Pfam; PF00135; COesterase; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
DR PROSITE; PS00122; CARBOXYLESTERASE_B_1; 1.
DR PROSITE; PS00941; CARBOXYLESTERASE_B_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361235};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022963};
KW Reference proteome {ECO:0000313|Proteomes:UP000009328};
KW Signal {ECO:0000256|RuleBase:RU361235}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|RuleBase:RU361235"
FT CHAIN 21..571
FT /note="Carboxylic ester hydrolase"
FT /evidence="ECO:0000256|RuleBase:RU361235"
FT /id="PRO_5005137203"
FT DOMAIN 29..540
FT /note="Carboxylesterase type B"
FT /evidence="ECO:0000259|Pfam:PF00135"
SQ SEQUENCE 571 AA; 64431 MW; 9CE9CACFC39C9AB3 CRC64;
MNWYLLYPCI ILYLSILSNA SSIIQKVHTP IVKLQNGTIS GNHLPRFNQD VFLGIPFAEP
PINELRFQPP QSYNTTWSGI KSFTEYGDAC YSATKSVSNA TQSEDCLTLN VVKPHGYYGE
KLPVVIWIHG GSFDHLSSSY SLFNGSFIVQ QSEILRKPIL FVSFNYRLNG FGFLNSNEIV
EKGWTNIGLR DQIKAVEWVH ENIESFGGDP NHLVLWGESA GGKSVGRLLN NNKYLGPYIK
GGILESGANV FVNVQKGVTT SNQESFDNVV RYFNCESNYN NTDALSCLQK VDADDLRKAF
NISNGIISRE FKFPSIDNDV VPDSGYNTVK AGNTTWNVPL LIGTNSDEGS YFTNVSIGSV
QESKDYLRTL YPNLKSNSID KLIALYPNKP GTITPTDPNY AKGPIKYPDS IGDQYKTLTT
LNSDVLYIAA TKITAQAYVN LNQPVYKYRF NIPNLETNGE DYYKGSTHGQ EVQYVFNNNE
SLKKSNEVNN KVYGISDTLS KLWIFFINDL NPNPRSHSER RGFEVEVPEW PLYNDGEQQV
VFDLKGFYVE RDIHRIEQFE FIESIIHELD G
//
