ID K0NGP1_DESTT Unreviewed; 462 AA.
AC K0NGP1;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE SubName: Full=RumA: predicted 23S rRNA mehtyltransferase {ECO:0000313|EMBL:CCK80406.1};
DE EC=2.1.1.- {ECO:0000313|EMBL:CCK80406.1};
GN Name=rumA {ECO:0000313|EMBL:CCK80406.1};
GN OrderedLocusNames=TOL2_C22450 {ECO:0000313|EMBL:CCK80406.1};
OS Desulfobacula toluolica (strain DSM 7467 / Tol2).
OC Bacteria; Thermodesulfobacteriota; Desulfobacteria; Desulfobacterales;
OC Desulfobacteraceae; Desulfobacula.
OX NCBI_TaxID=651182 {ECO:0000313|EMBL:CCK80406.1, ECO:0000313|Proteomes:UP000007347};
RN [1] {ECO:0000313|EMBL:CCK80406.1, ECO:0000313|Proteomes:UP000007347}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 7467 / Tol2 {ECO:0000313|Proteomes:UP000007347};
RX PubMed=23088741; DOI=10.1111/j.1462-2920.2012.02885.x;
RA Wohlbrand L., Jacob J.H., Kube M., Mussmann M., Jarling R., Beck A.,
RA Amann R., Wilkes H., Reinhardt R., Rabus R.;
RT "Complete genome, catabolic sub-proteomes and key-metabolites of
RT Desulfobacula toluolica Tol2, a marine, aromatic compound-degrading,
RT sulfate-reducing bacterium.";
RL Environ. Microbiol. 15:1334-55(2013).
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RNA M5U methyltransferase family. {ECO:0000256|PROSITE-
CC ProRule:PRU01024}.
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DR EMBL; FO203503; CCK80406.1; -; Genomic_DNA.
DR RefSeq; WP_014957718.1; NC_018645.1.
DR AlphaFoldDB; K0NGP1; -.
DR STRING; 651182.TOL2_C22450; -.
DR KEGG; dto:TOL2_C22450; -.
DR PATRIC; fig|651182.5.peg.2653; -.
DR HOGENOM; CLU_014689_8_1_7; -.
DR OrthoDB; 9804590at2; -.
DR Proteomes; UP000007347; Chromosome.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR GO; GO:0034470; P:ncRNA processing; IEA:UniProt.
DR GO; GO:0009451; P:RNA modification; IEA:UniProt.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 2.40.50.1070; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR030390; MeTrfase_TrmA_AS.
DR InterPro; IPR030391; MeTrfase_TrmA_CS.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR002792; TRAM_dom.
DR InterPro; IPR010280; U5_MeTrfase_fam.
DR NCBIfam; TIGR00479; rumA; 1.
DR PANTHER; PTHR11061; RNA M5U METHYLTRANSFERASE; 1.
DR PANTHER; PTHR11061:SF30; TRNA (URACIL(54)-C(5))-METHYLTRANSFERASE; 1.
DR Pfam; PF01938; TRAM; 1.
DR Pfam; PF05958; tRNA_U5-meth_tr; 2.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS51687; SAM_MT_RNA_M5U; 1.
DR PROSITE; PS50926; TRAM; 1.
DR PROSITE; PS01230; TRMA_1; 1.
DR PROSITE; PS01231; TRMA_2; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01024}; Reference proteome {ECO:0000313|Proteomes:UP000007347};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01024};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01024}.
FT DOMAIN 2..60
FT /note="TRAM"
FT /evidence="ECO:0000259|PROSITE:PS50926"
FT ACT_SITE 420
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10015"
FT ACT_SITE 420
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 298
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 327
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 348
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
FT BINDING 393
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01024"
SQ SEQUENCE 462 AA; 52752 MW; 8932438E3FB8DCA7 CRC64;
MSIKKRKAYE LEITDLAFGG RGLAKPDGFP VFIDRCIPGD VVFAKITKKK KSWAEGKLIK
ILQESSLRKE GKCQYCNFCG GCKWQQIGYD LQLEYKKRHV TESMQHIGGL KDVLVKDVIP
SDNIYEYRNK MEFSCSSKRW LLPWELENEE IKKDFGIGLH VPGTFDKVID IKRCEIMPDL
GNQILDDVRN FIKESDLPAY NLRTHEGFWR FLMLRHSVAF DTWMVNIVTT FKQMDVVRDL
ASVLTQNHPQ IKSVMNNITD AKSGVSIGKE EICLYGKDHI KERLGKFVFK ISPNSFFQTN
TRSCENLYAK VSEYAALTGK ETVIDLYSGT GTIPIWLSDQ AKMVYGIEIV KSAVVDAKLN
AQLNGIENCE FFEGDIKDVL PGLKQKPDVM IIDPPRVGMH KDVVQQVLYI SPEKIVYVSC
NPATLARDLE MLAPEYDIKE IQPVDMFPHT YHIESVALLL KK
//
