ID K1E3F0_9MICO Unreviewed; 716 AA.
AC K1E3F0;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 56.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=B277_15892 {ECO:0000313|EMBL:EKA59877.1};
OS Janibacter hoylei PVAS-1.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Intrasporangiaceae;
OC Janibacter.
OX NCBI_TaxID=1210046 {ECO:0000313|EMBL:EKA59877.1, ECO:0000313|Proteomes:UP000004474};
RN [1] {ECO:0000313|EMBL:EKA59877.1, ECO:0000313|Proteomes:UP000004474}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PVAS-1 {ECO:0000313|EMBL:EKA59877.1,
RC ECO:0000313|Proteomes:UP000004474};
RX PubMed=23144385; DOI=10.1128/JB.01728-12;
RA Pawar S.P., Dhotre D.P., Shetty S.A., Chowdhury S.P., Chaudhari B.L.,
RA Shouche Y.S.;
RT "Genome Sequence of Janibacter hoylei MTCC8307, Isolated from the
RT Stratospheric Air.";
RL J. Bacteriol. 194:6629-6630(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKA59877.1}.
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DR EMBL; ALWX01000096; EKA59877.1; -; Genomic_DNA.
DR RefSeq; WP_007929880.1; NZ_PIPF01000012.1.
DR AlphaFoldDB; K1E3F0; -.
DR STRING; 1210046.B277_15892; -.
DR PATRIC; fig|1210046.3.peg.3046; -.
DR eggNOG; COG0210; Bacteria.
DR Proteomes; UP000004474; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 1.10.150.80; HRDC domain; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 3.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR010997; HRDC-like_sf.
DR InterPro; IPR002121; HRDC_dom.
DR InterPro; IPR044876; HRDC_dom_sf.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF45; ATP-DEPENDENT DNA HELICASE UVRD2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF00570; HRDC; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SMART; SM00341; HRDC; 1.
DR SUPFAM; SSF47819; HRDC-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS50967; HRDC; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}.
FT DOMAIN 15..296
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 297..546
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT DOMAIN 628..708
FT /note="HRDC"
FT /evidence="ECO:0000259|PROSITE:PS50967"
FT REGION 579..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 582..596
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 36..43
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 716 AA; 77280 MW; AF5D44C1731537E6 CRC64;
MIDVTGPQSA DDILDALDPE QREVAANPCG PMVVLAGAGT GKTRAITHRI AYGVRSGAYQ
PQRVLAVTFT ARAAGEMRTR LRDLGVVGVQ ARTFHAAALR QLHYFWPQAI GGAAPEVMAQ
KIPAVAEAAS RMGMRLDRSE LRDVASEIEW SKVSLLTPET YAASARRARR EPPGMDHTAM
ARLISTYEDV KGERGVIDFE DVLLLTVGIL EEREDIARTV RDQYRHFVVD EYQDVNALQQ
RLLDLWLGGR GDICVVGDPA QTIYSFTGAT PEHLLSFTRH HPGASTVRLV RNYRSTPPVL
ALANRMLTDT NGQRRGGSVE LVPQREGGTP PQLTALDDDP AEAAWVAAKV KELVGQGRPL
REIAVLFRTN GQSEAFESAL ADADVPYLVR GGERFFSRSE VREGILLLRG AARTDDGSKP
LPAFVGDVLG GMGWHEDAPS GTGAVRSRWE SLKALVDLAA RLAAASPEAR LPELVRDLDE
RIAAQHAPDV EGVTLASLHA AKGLEWDVVF LVGCSDGLLP ITMAEGVEEI EEERRLMYVG
VTRAREVLHL SWSRARNPGA RSSRRVSRFL DHTASILGEG ARSTPKRSSS SRGSGPLKPS
RPKVCKVCGT ELATATERAS GRCPDCPPTY DEATFERLRA WRLAVSRAAS VPAFVVFTDA
TLEAIAEAEP TGQRDLSAIS GVGPRKLSLY ADQVLAVVGG ADPDEVAAAS AGEPEA
//
