ID K1QZF2_CRAGI Unreviewed; 1628 AA.
AC K1QZF2;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 24-JAN-2024, entry version 36.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN ORFNames=CGI_10011702 {ECO:0000313|EMBL:EKC39023.1};
OS Crassostrea gigas (Pacific oyster) (Crassostrea angulata).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Bivalvia;
OC Autobranchia; Pteriomorphia; Ostreida; Ostreoidea; Ostreidae; Crassostrea.
OX NCBI_TaxID=29159 {ECO:0000313|EMBL:EKC39023.1};
RN [1] {ECO:0000313|EMBL:EKC39023.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=05x7-T-G4-1.051#20 {ECO:0000313|EMBL:EKC39023.1};
RX PubMed=22992520; DOI=10.1038/nature11413;
RA Zhang G., Fang X., Guo X., Li L., Luo R., Xu F., Yang P., Zhang L.,
RA Wang X., Qi H., Xiong Z., Que H., Xie Y., Holland P.W., Paps J., Zhu Y.,
RA Wu F., Chen Y., Wang J., Peng C., Meng J., Yang L., Liu J., Wen B.,
RA Zhang N., Huang Z., Zhu Q., Feng Y., Mount A., Hedgecock D., Xu Z., Liu Y.,
RA Domazet-Loso T., Du Y., Sun X., Zhang S., Liu B., Cheng P., Jiang X.,
RA Li J., Fan D., Wang W., Fu W., Wang T., Wang B., Zhang J., Peng Z., Li Y.,
RA Li N., Wang J., Chen M., He Y., Tan F., Song X., Zheng Q., Huang R.,
RA Yang H., Du X., Chen L., Yang M., Gaffney P.M., Wang S., Luo L., She Z.,
RA Ming Y., Huang W., Zhang S., Huang B., Zhang Y., Qu T., Ni P., Miao G.,
RA Wang J., Wang Q., Steinberg C.E., Wang H., Li N., Qian L., Zhang G., Li Y.,
RA Yang H., Liu X., Wang J., Yin Y., Wang J.;
RT "The oyster genome reveals stress adaptation and complexity of shell
RT formation.";
RL Nature 490:49-54(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; JH817764; EKC39023.1; -; Genomic_DNA.
DR HOGENOM; CLU_243281_0_0_1; -.
DR InParanoid; K1QZF2; -.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 2.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF29; ENDOGLUCANASE; 1.
DR Pfam; PF00759; Glyco_hydro_9; 2.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 2.
DR PROSITE; PS00592; GH9_2; 2.
DR PROSITE; PS00698; GH9_3; 2.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059}.
FT DOMAIN 416..839
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT DOMAIN 1141..1564
FT /note="Glycoside hydrolase family 9"
FT /evidence="ECO:0000259|Pfam:PF00759"
FT ACT_SITE 774
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 818
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 827
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 1499
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 1543
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 1552
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 1628 AA; 180030 MW; 36F87B13765E22A2 CRC64;
MRPAGSVRLL RADDTRLSGL SDLWWEGRTS GGSSEGAGDF PWLDFCLGVP AFAERSLALG
IVEGSCSTYT KEPSKITITV FVILRITLPL QACVVFLVLA VSAYRASAAY NTPVQRLTDW
WDGKYHYFHG FCDLPIDKKY RMRRVKVNLV LDTPSESIQI WKLKVIKKNC DGTEYLMRNK
YEIHKGVERA DFILKYTSQQ IPTGMCFTNL PSPKTTKAPS TPTPTTPKPT TSSPAISTSQ
STGNDVYQVP VVKVQEPWGG GSSWTFKGRC TLKISDTWTP ATLPVDLIFD QETSNLQIWK
MNVLSSSLNG MLYELTNEHR VSPGQLEITF IVDYSGTINS HPQGFCIHVS KITPGSTQPT
SFTSTTTTAS PSKTTTAQPS TTITKAASST TGAQSTTTGG TLGETTPVPS NTAHNYGQVL
EKSILFYEAQ QSGDLPPTHR INWRGDSALG DSGIYGEDLT GGWYDAGDGV KFNLPMSASV
INLAVGLYKW RDAYSASNQL QHMYDSIKWP LDYFLKCWQN STQIYYAQVG NGDTDHAIWT
RPEDITISRP PYYLDSSKRG SDVAGGTAAA LAAGALAFLS EDSSYSSKLL ESAESLYAFA
KAHQGTYNTD IPEGAKFYSS SEYRDEMCVS AALLYLATNK TGYRSDAITF YNSYSPGRWA
YDWDDKTMLC DVLLYEITQE TKYKSAVENF VTSYMPGGSV QYTPCGLAWR LEWGSLRYSA
NAAFIALLAA DAGIGNTANY KQWAMSQIHY MLGDNADAFS YVIGYGSNYP LKPHHRASSC
DSPPAVCDWN DYNSPDPNPH VLQGALVGGP DQYDNYEDAR DNFRTNEVAC DYNAGFQSAV
AGLLHFAVNV LGSNKPVQEM FTFLSSNVQD GRTFPLRYFV LLVLAVAAYR TSAYNTAVQP
STDWWDGPFH YFQGYCDLPI DKSYPTGGVT VDLLLDTPSE SIQIWKLDIS TVNNDGTEYY
MYNKYEIHQG VERVGFILKY TSNNIPYGIC VTSLTSTQTT STTTTTTTTK APTTTTTVTP
APTTSSPTSS TSQSTAGNDV YQVPVVKVEG PWGGGSSWTF KGKCTFEISA TWAIGTLPVD
LIFNQETSNL QIWKMNILSN ALNGMLYELN NKYWVSPGTL EFNFMVDYSG TINSYPQAHN
YGLVLEKSIL FYEAQQSGDL PPTHRINWRG DSALGDSGIY GEDLTGGWYD AGDTVKFNLP
MSASVINLAV GLYKWKDAYS ASNQLQHMYD SIKWPLDYFL KCWRNSAQIY YAQVGNGDTD
HAIWTRPEDM TISRPPYYLN SSKTGSDVAG GTAAALAAGA LAFMGEDSSY STSLLESAES
LYAFAKAHQG TYNTYIPEGA KFYSSSEYRD EMCVSAALLY LATNKTGYRS DAITFYNSYS
PGRWAYDWDD KTMLCDILLY EITQETKYKS AVENFVTSYM PGGSVQYTPC GLAWRLEWGS
LRYSANAAFI ALLAADAGIG NTANYKQWAM SQIHYMLGDN ANDFSYVIGY GSNYPLKPHH
RASSCDYPPE VCDWNDYNSP DPNPHVLQGA LVGGPDQYDN YEDARDNFRT NEVACDYNAG
FQSAVAGTTN RSVKNEKYNG QYNASISTCH HHRRRRRRCR RNHLLFNFPG LLHFAVNGNL
PAAPTPAC
//
