ID K2SBK4_MACPH Unreviewed; 834 AA.
AC K2SBK4;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 45.
DE RecName: Full=Nitrate reductase [NADPH] {ECO:0000256|ARBA:ARBA00015499};
DE EC=1.7.1.3 {ECO:0000256|ARBA:ARBA00012673};
DE Flags: Fragment;
GN ORFNames=MPH_08578 {ECO:0000313|EMBL:EKG14250.1};
OS Macrophomina phaseolina (strain MS6) (Charcoal rot fungus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae;
OC Macrophomina.
OX NCBI_TaxID=1126212 {ECO:0000313|EMBL:EKG14250.1, ECO:0000313|Proteomes:UP000007129};
RN [1] {ECO:0000313|EMBL:EKG14250.1, ECO:0000313|Proteomes:UP000007129}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MS6 {ECO:0000313|EMBL:EKG14250.1,
RC ECO:0000313|Proteomes:UP000007129};
RX PubMed=22992219; DOI=10.1186/1471-2164-13-493;
RA Islam M.S., Haque M.S., Islam M.M., Emdad E.M., Halim A., Hossen Q.M.M.,
RA Hossain M.Z., Ahmed B., Rahim S., Rahman M.S., Alam M.M., Hou S., Wan X.,
RA Saito J.A., Alam M.;
RT "Tools to kill: Genome of one of the most destructive plant pathogenic
RT fungi Macrophomina phaseolina.";
RL BMC Genomics 13:493-493(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + NADP(+) + nitrite = H(+) + NADPH + nitrate;
CC Xref=Rhea:RHEA:19061, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16301, ChEBI:CHEBI:17632, ChEBI:CHEBI:57783,
CC ChEBI:CHEBI:58349; EC=1.7.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00000195};
CC -!- COFACTOR:
CC Name=Mo-molybdopterin; Xref=ChEBI:CHEBI:71302;
CC Evidence={ECO:0000256|ARBA:ARBA00001924};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKG14250.1}.
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DR EMBL; AHHD01000363; EKG14250.1; -; Genomic_DNA.
DR AlphaFoldDB; K2SBK4; -.
DR STRING; 1126212.K2SBK4; -.
DR VEuPathDB; FungiDB:MPH_08578; -.
DR eggNOG; KOG0535; Eukaryota.
DR eggNOG; KOG0537; Eukaryota.
DR HOGENOM; CLU_003827_4_2_1; -.
DR InParanoid; K2SBK4; -.
DR OrthoDB; 1239at2759; -.
DR Proteomes; UP000007129; Unassembled WGS sequence.
DR GO; GO:0030151; F:molybdenum ion binding; IEA:InterPro.
DR GO; GO:0050464; F:nitrate reductase (NADPH) activity; IEA:UniProtKB-EC.
DR Gene3D; 2.60.40.650; -; 1.
DR Gene3D; 3.10.120.10; Cytochrome b5-like heme/steroid binding domain; 1.
DR Gene3D; 3.90.420.10; Oxidoreductase, molybdopterin-binding domain; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR InterPro; IPR008333; Cbr1-like_FAD-bd_dom.
DR InterPro; IPR001199; Cyt_B5-like_heme/steroid-bd.
DR InterPro; IPR036400; Cyt_B5-like_heme/steroid_sf.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR005066; MoCF_OxRdtse_dimer.
DR InterPro; IPR008335; Mopterin_OxRdtase_euk.
DR InterPro; IPR000572; OxRdtase_Mopterin-bd_dom.
DR InterPro; IPR036374; OxRdtase_Mopterin-bd_sf.
DR InterPro; IPR017938; Riboflavin_synthase-like_b-brl.
DR PANTHER; PTHR19372:SF7; SULFITE OXIDASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR19372; SULFITE REDUCTASE; 1.
DR Pfam; PF00173; Cyt-b5; 1.
DR Pfam; PF00970; FAD_binding_6; 1.
DR Pfam; PF03404; Mo-co_dimer; 1.
DR Pfam; PF00174; Oxidored_molyb; 1.
DR PRINTS; PR00407; EUMOPTERIN.
DR SMART; SM01117; Cyt-b5; 1.
DR SUPFAM; SSF55856; Cytochrome b5-like heme/steroid binding domain; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF56524; Oxidoreductase molybdopterin-binding domain; 1.
DR SUPFAM; SSF63380; Riboflavin synthase domain-like; 1.
DR PROSITE; PS50255; CYTOCHROME_B5_2; 1.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000007129}.
FT DOMAIN 670..748
FT /note="Cytochrome b5 heme-binding"
FT /evidence="ECO:0000259|PROSITE:PS50255"
FT REGION 118..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..234
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 118..162
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 834
FT /evidence="ECO:0000313|EMBL:EKG14250.1"
SQ SEQUENCE 834 AA; 95094 MW; 54D3F573BB32B4A5 CRC64;
MPPVLWTVKV QNHPGSSADD IAKEPDWGAG LRHRIGFKND QDRVPGITHL EDEYDEDVEE
AREELVELNT RSEGPQLINF RDAMGNQKDL HRWTNESRSI GWRYVLEASE HWIKNTEDWP
ANAKKRQQAK NQRMKEEDRG GQQEHEWKRS QGENKHHNAY AGVEESGYDS GIEQSGQAGN
QESDQGKLRK KYSPQEITLL RSLRHEKEYI SQLKCNNGKR RSPQQHNKTP IFINEQDQCS
PDNWLPRSSD LIRLTGKHPL NAEAPLSHLF DAGLITPNEL HYVRNHGAVP RLLWELHKLD
IQGGHMTLSM DDLKDKFATI NIPIALACDG NRRKELHMIK KSKGFSWGAG AVGCAYWKGP
LLRDVLLSAG IPEIMPGGDG KRYWVHFEGA DEPSEAKYAT CLPFQYVMDP TNDVILAYEM
NDLPLPPDHG YPVRLMVPGY VGGRCVKWLK KIWISDKEND SHHHVWDNRL LPSFVTEKDG
EFAEAMFRHP DTACNEQNLN SVIVKPAHGE RIPLTTARKG HSYRIAGYAY DGGGHEVQRV
EVSLDDGETW LYCIRQFPDY PIRHGNKFWT WLHWHVDVEL SHLLRSKSIT VRCFNVFKNT
QPEKPSWNIM GVMNNCWYVV KPSIVDGEET DVPSILFEHP VEPGTGAGGW MKPSTESRIE
NAKQEAGTPQ KQFTRQEIEK HDKEDDCWIV IDGKVYDATS LLSWHPGGKA AVLGHAGKLH
QETSDEFASI HDGYAYQKLK ECVLGIVTEK AADFIKKNDE AAAKDEASSQ DNDDQLTLQK
HRWLPVRLID REDISKDTRT YTFALPDGKS VLGLGTCQHV QIGFHMKDRM LIRS
//
