ID K3XEW2_SETIT Unreviewed; 741 AA.
AC K3XEW2;
DT 28-NOV-2012, integrated into UniProtKB/TrEMBL.
DT 28-NOV-2012, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=threonine--tRNA ligase {ECO:0000256|ARBA:ARBA00013163};
DE EC=6.1.1.3 {ECO:0000256|ARBA:ARBA00013163};
DE AltName: Full=Threonyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00031900};
GN Name=101764893 {ECO:0000313|EnsemblPlants:KQL04697};
GN ORFNames=SETIT_5G129000v2 {ECO:0000313|EMBL:RCV24969.1};
OS Setaria italica (Foxtail millet) (Panicum italicum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Panicodae; Paniceae; Cenchrinae; Setaria.
OX NCBI_TaxID=4555 {ECO:0000313|EMBL:RCV24969.1};
RN [1] {ECO:0000313|EMBL:RCV24969.1, ECO:0000313|Proteomes:UP000004995}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Yugu1 {ECO:0000313|EnsemblPlants:KQL04697,
RC ECO:0000313|Proteomes:UP000004995}, and Yugu1
RC {ECO:0000313|EMBL:RCV24969.1};
RX PubMed=22580951; DOI=10.1038/nbt.2196;
RA Bennetzen J.L., Schmutz J., Wang H., Percifield R., Hawkins J.,
RA Pontaroli A.C., Estep M., Feng L., Vaughn J.N., Grimwood J., Jenkins J.,
RA Barry K., Lindquist E., Hellsten U., Deshpande S., Wang X., Wu X.,
RA Mitros T., Triplett J., Yang X., Ye C.Y., Mauro-Herrera M., Wang L., Li P.,
RA Sharma M., Sharma R., Ronald P.C., Panaud O., Kellogg E.A., Brutnell T.P.,
RA Doust A.N., Tuskan G.A., Rokhsar D., Devos K.M.;
RT "Reference genome sequence of the model plant Setaria.";
RL Nat. Biotechnol. 30:555-561(2012).
RN [2] {ECO:0000313|EMBL:RCV24969.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Yugu1 {ECO:0000313|EMBL:RCV24969.1};
RA Noorani M.;
RL Submitted (JUL-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:KQL04697}
RP IDENTIFICATION.
RC STRAIN=Yugu1 {ECO:0000313|EnsemblPlants:KQL04697};
RG EnsemblPlants;
RL Submitted (AUG-2018) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonine + tRNA(Thr) = AMP + diphosphate + H(+) + L-
CC threonyl-tRNA(Thr); Xref=Rhea:RHEA:24624, Rhea:RHEA-COMP:9670,
CC Rhea:RHEA-COMP:9704, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57926, ChEBI:CHEBI:78442,
CC ChEBI:CHEBI:78534, ChEBI:CHEBI:456215; EC=6.1.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00000070};
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226}.
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DR EMBL; AGNK02002911; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CM003532; RCV24969.1; -; Genomic_DNA.
DR RefSeq; XP_004968434.1; XM_004968377.2.
DR AlphaFoldDB; K3XEW2; -.
DR STRING; 4555.K3XEW2; -.
DR EnsemblPlants; KQL04697; KQL04697; SETIT_000429mg.
DR GeneID; 101764893; -.
DR Gramene; KQL04697; KQL04697; SETIT_000429mg.
DR KEGG; sita:101764893; -.
DR eggNOG; KOG1637; Eukaryota.
DR HOGENOM; CLU_008554_0_1_1; -.
DR InParanoid; K3XEW2; -.
DR OMA; NHRKLGS; -.
DR OrthoDB; 1119631at2759; -.
DR Proteomes; UP000004995; Chromosome V.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004829; F:threonine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0006435; P:threonyl-tRNA aminoacylation; IBA:GO_Central.
DR CDD; cd01667; TGS_ThrRS; 1.
DR CDD; cd00860; ThrRS_anticodon; 1.
DR CDD; cd00771; ThrRS_core; 1.
DR Gene3D; 3.10.20.30; -; 1.
DR Gene3D; 3.40.50.800; Anticodon-binding domain; 1.
DR HAMAP; MF_00184; Thr_tRNA_synth; 1.
DR InterPro; IPR002314; aa-tRNA-synt_IIb.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004154; Anticodon-bd.
DR InterPro; IPR036621; Anticodon-bd_dom_sf.
DR InterPro; IPR012675; Beta-grasp_dom_sf.
DR InterPro; IPR004095; TGS.
DR InterPro; IPR012676; TGS-like.
DR InterPro; IPR002320; Thr-tRNA-ligase_IIa.
DR InterPro; IPR018163; Thr/Ala-tRNA-synth_IIc_edit.
DR InterPro; IPR047246; ThrRS_anticodon.
DR InterPro; IPR033728; ThrRS_core.
DR InterPro; IPR012947; tRNA_SAD.
DR NCBIfam; TIGR00418; thrS; 1.
DR PANTHER; PTHR11451:SF46; THREONINE--TRNA LIGASE; 1.
DR PANTHER; PTHR11451; THREONINE-TRNA LIGASE; 1.
DR Pfam; PF03129; HGTP_anticodon; 1.
DR Pfam; PF02824; TGS; 1.
DR Pfam; PF00587; tRNA-synt_2b; 1.
DR Pfam; PF07973; tRNA_SAD; 1.
DR PRINTS; PR01047; TRNASYNTHTHR.
DR SMART; SM00863; tRNA_SAD; 1.
DR SUPFAM; SSF52954; Class II aaRS ABD-related; 1.
DR SUPFAM; SSF55681; Class II aaRS and biotin synthetases; 1.
DR SUPFAM; SSF81271; TGS-like; 1.
DR SUPFAM; SSF55186; ThrRS/AlaRS common domain; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
DR PROSITE; PS51880; TGS; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000004995}.
FT DOMAIN 105..167
FT /note="TGS"
FT /evidence="ECO:0000259|PROSITE:PS51880"
FT DOMAIN 373..639
FT /note="Aminoacyl-transfer RNA synthetases class-II family
FT profile"
FT /evidence="ECO:0000259|PROSITE:PS50862"
SQ SEQUENCE 741 AA; 84395 MW; EED77376FE0E5614 CRC64;
MLVCLRRGLS LVRQHTPRFL PSPPLRPARL FLHHLPAADG MGESSAAGKD AKGKGKAKAK
APAAASALVV TRDDSYLEAV TQTRIRKFEE IQARQALERL NIGGELIKVT LPDGAIKEGK
KLITTPMDIA KEISSGLAAS CLIAKVDETL WDMGRPLEGD CKLQLFKFDS NEGRDTFWHS
SAHILGESLE RVYGCKLCIG PCTTRGEGFY YDAYYNDLTL NEEHFGIIES QAQKAVAEKQ
PFERIEVSRA EALEMFAENK FKVEIINELP EDKTITVYRC GPLVDLCRGP HIPNTSFVKA
FACLKASSSY WRGKADRESL QRVYGISFPD SKRLKEYKHF LEEAKKRDHR TLGLAQELFF
FHPLSPGSCF FLPHGARVYN KLMDFMRQQY RDRGYQEVLS PNIYNMQLWE TSGHAANYKE
NMFVFEIEKQ EFGLKPMNCP GHCLMFNNRV RSYRELPLRM ADFGVLHRNE LSGALTGLTR
VRRFQQDDAH IFCREDQIKD EVKGVLEFIN YVYEIFGFKY ELELSTRPEK YLGDIETWNK
PEQQLTEALN EFGMPWQINE GDGAFYGPKI DIGVFDALKR KFQCATLQLD FQLPLRFKLS
YSAEDEAKIE RPVMIHRAIL GSVERMFAIL LEHYNGKWPL WLSPRQAIVC SVSSGSVEYA
KQVLARLHEA GFHVDIDMSD RTIQKKVREA QVAQFNYTLV VGAQEAETGN VSVRVRGSDG
LATMSLDGLI TRFREEIAAF K
//
