ID K4LIK3_THEPS Unreviewed; 578 AA.
AC K4LIK3;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE SubName: Full=Formate hydrogenlyase subunit 5 {ECO:0000313|EMBL:AFV12801.1};
GN Name=hycE {ECO:0000313|EMBL:AFV12801.1};
GN OrderedLocusNames=Tph_c26330 {ECO:0000313|EMBL:AFV12801.1};
OS Thermacetogenium phaeum (strain ATCC BAA-254 / DSM 26808 / PB).
OC Bacteria; Bacillota; Clostridia; Thermoanaerobacterales;
OC Thermoanaerobacteraceae; Thermacetogenium.
OX NCBI_TaxID=1089553 {ECO:0000313|EMBL:AFV12801.1, ECO:0000313|Proteomes:UP000000467};
RN [1] {ECO:0000313|EMBL:AFV12801.1, ECO:0000313|Proteomes:UP000000467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-254 / DSM 26808 / PB
RC {ECO:0000313|Proteomes:UP000000467};
RX PubMed=23259483; DOI=10.1186/1471-2164-13-723;
RA Oehler D., Poehlein A., Leimbach A., Muller N., Daniel R., Gottschalk G.,
RA Schink B.;
RT "Genome-guided analysis of physiological and morphological traits of the
RT fermentative acetate oxidizer Thermacetogenium phaeum.";
RL BMC Genomics 13:723-723(2012).
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
CC -!- COFACTOR:
CC Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC Evidence={ECO:0000256|PIRSR:PIRSR601501-1};
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DR EMBL; CP003732; AFV12801.1; -; Genomic_DNA.
DR RefSeq; WP_015051661.1; NZ_KI912609.1.
DR AlphaFoldDB; K4LIK3; -.
DR STRING; 1089553.Tph_c26330; -.
DR KEGG; tpz:Tph_c26330; -.
DR eggNOG; COG3261; Bacteria.
DR eggNOG; COG3262; Bacteria.
DR HOGENOM; CLU_015134_3_1_9; -.
DR OrthoDB; 9801496at2; -.
DR Proteomes; UP000000467; Chromosome.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR GO; GO:0008137; F:NADH dehydrogenase (ubiquinone) activity; IEA:InterPro.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR GO; GO:0048038; F:quinone binding; IEA:InterPro.
DR Gene3D; 1.10.645.10; Cytochrome-c3 Hydrogenase, chain B; 1.
DR Gene3D; 3.30.460.80; NADH:ubiquinone oxidoreductase, 30kDa subunit; 1.
DR InterPro; IPR001135; NADH_Q_OxRdtase_suD.
DR InterPro; IPR037232; NADH_quin_OxRdtase_su_C/D-like.
DR InterPro; IPR001268; NADH_UbQ_OxRdtase_30kDa_su.
DR InterPro; IPR001501; Ni-dep_hyd_lsu.
DR InterPro; IPR029014; NiFe-Hase_large.
DR PANTHER; PTHR43485:SF1; FORMATE HYDROGENLYASE SUBUNIT 5-RELATED; 1.
DR PANTHER; PTHR43485; HYDROGENASE-4 COMPONENT G; 1.
DR Pfam; PF00329; Complex1_30kDa; 1.
DR Pfam; PF00346; Complex1_49kDa; 2.
DR Pfam; PF00374; NiFeSe_Hases; 1.
DR SUPFAM; SSF56762; HydB/Nqo4-like; 1.
DR SUPFAM; SSF143243; Nqo5-like; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|PIRSR:PIRSR601501-1};
KW Lyase {ECO:0000313|EMBL:AFV12801.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR601501-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR601501-1};
KW Nickel {ECO:0000256|PIRSR:PIRSR601501-1};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000000467}.
FT DOMAIN 42..161
FT /note="NADH:ubiquinone oxidoreductase 30kDa subunit"
FT /evidence="ECO:0000259|Pfam:PF00329"
FT DOMAIN 304..472
FT /note="NADH-quinone oxidoreductase subunit D"
FT /evidence="ECO:0000259|Pfam:PF00346"
FT DOMAIN 473..545
FT /note="NADH-quinone oxidoreductase subunit D"
FT /evidence="ECO:0000259|Pfam:PF00346"
FT BINDING 229
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 249
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 252
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 252
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 539
FT /ligand="Ni(2+)"
FT /ligand_id="ChEBI:CHEBI:49786"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
FT BINDING 542
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR601501-1"
SQ SEQUENCE 578 AA; 66091 MW; 7EBDA5391C6DE755 CRC64;
MNPQTEPVKS NGKKEEVESL RSGFGQAILD ETWQTPDQVT LTVDLDYLPQ VVAAAYYRLG
GWLSSVIGND ERRLNGAFAL YYVLSMEHER PEENFWLTIK ALVPPEKLEF PSVTPLVPAA
AWYERDVRDL LGIHPVNHPD PRRLVLPDDW PDGLHPLRKD AMDYRFRPEA ATDEETYEFV
NVEGEGIMEV PLGPLHVTSD EPGHFRLFVD GETIVDADYR LFYCHRGLEK LAENRMDYDQ
VHFLAERICG ICGYAHSIAY TTAVERAIGL EVPERARYLR TILLEVERLH SHLLNLGLAC
HFTGFDSGFM QFFRVREKAM RIAEILSGAR KTYGVNLIGG VRRDILKEER DQVVDLLAEC
RRETDDLIAI LLNTPNLRKR TVGVGVLDRK VARDFSPVGP NVRGSGFQRD TRSDHPYLAY
DSIPWEVVTA EGCDVFSRVL VRAKEVYETF NIIQHCLQEL PGGPVLVEGF TYKPHRYALG
YVEAPRGEDI HWVMTCDNQK LYRWRPRASS YNNWPAIRFM LRGNAISDAP LIIASIDPCY
SCTERVTVVD IKKKKAKDIS YKELERYCIE KKDSPLKG
//
