ID K5BE49_MYCHD Unreviewed; 450 AA.
AC K5BE49;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Glutamate synthase [NADPH] large chain {ECO:0000313|EMBL:VCT92199.1};
DE EC=1.4.1.13 {ECO:0000313|EMBL:VCT92199.1};
DE SubName: Full=Nitronate monooxygenase family protein {ECO:0000313|EMBL:EKF21986.1};
GN Name=gltA {ECO:0000313|EMBL:VCT92199.1};
GN ORFNames=C731_4052 {ECO:0000313|EMBL:EKF21986.1}, MHAS_03925
GN {ECO:0000313|EMBL:VCT92199.1};
OS Mycolicibacterium hassiacum (strain DSM 44199 / CIP 105218 / JCM 12690 /
OS 3849) (Mycobacterium hassiacum).
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=1122247 {ECO:0000313|EMBL:EKF21986.1, ECO:0000313|Proteomes:UP000006265};
RN [1] {ECO:0000313|EMBL:EKF21986.1, ECO:0000313|Proteomes:UP000006265}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44199 {ECO:0000313|EMBL:EKF21986.1}, and DSM 44199 / CIP
RC 105218 / JCM 12690 / 3849 {ECO:0000313|Proteomes:UP000006265};
RX PubMed=23209251; DOI=10.1128/JB.01880-12;
RA Tiago I., Maranha A., Mendes V., Alarico S., Moynihan P.J., Clarke A.J.,
RA Macedo-Ribeiro S., Pereira P.J., Empadinhas N.;
RT "Genome sequence of Mycobacterium hassiacum DSM 44199, a rare source of
RT heat-stable mycobacterial proteins.";
RL J. Bacteriol. 194:7010-7011(2012).
RN [2] {ECO:0000313|EMBL:VCT92199.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Mhassiacum {ECO:0000313|EMBL:VCT92199.1};
RA Peiro R., Begona, Cbmso G., Lopez M., Gonzalez S., Sacristan E.,
RA Castillo E.;
RL Submitted (OCT-2018) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|Proteomes:UP000268660}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 44199 / CIP 105218 / JCM 12690 / 3849
RC {ECO:0000313|Proteomes:UP000268660};
RX PubMed=30701245; DOI=10.1128/mra.01522-18;
RA Sanchez M., Blesa A., Sacristan-Horcajada E., Berenguer J.;
RT "Complete genome sequence of Mycolicibacterium hassiacum DSM 44199.";
RL Microbiol. Resour. Announc. 8:e01522-e01522(2019).
CC -!- SIMILARITY: Belongs to the glutamate synthase family.
CC {ECO:0000256|ARBA:ARBA00009716, ECO:0000256|PIRNR:PIRNR006429}.
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DR EMBL; AMRA01000107; EKF21986.1; -; Genomic_DNA.
DR EMBL; LR026975; VCT92199.1; -; Genomic_DNA.
DR RefSeq; WP_005630905.1; NZ_LR026975.1.
DR AlphaFoldDB; K5BE49; -.
DR STRING; 1122247.GCA_000379865_01832; -.
DR KEGG; mhas:MHAS_03925; -.
DR PATRIC; fig|1122247.3.peg.3888; -.
DR eggNOG; COG0069; Bacteria.
DR OrthoDB; 9758182at2; -.
DR Proteomes; UP000006265; Unassembled WGS sequence.
DR Proteomes; UP000268660; Chromosome 1.
DR GO; GO:0004355; F:glutamate synthase (NADPH) activity; IEA:UniProtKB-EC.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0006537; P:glutamate biosynthetic process; IEA:InterPro.
DR CDD; cd02808; GltS_FMN; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR024188; GltB.
DR InterPro; IPR043578; GltB_archl_type.
DR InterPro; IPR002932; Glu_synthdom.
DR PANTHER; PTHR43819:SF1; ARCHAEAL GLUTAMATE SYNTHASE [NADPH]; 1.
DR PANTHER; PTHR43819; ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]; 1.
DR Pfam; PF01645; Glu_synthase; 2.
DR PIRSF; PIRSF500061; GOGAT_lg2_archl; 1.
DR PIRSF; PIRSF006429; GOGAT_lg_2; 1.
DR SUPFAM; SSF51395; FMN-linked oxidoreductases; 1.
PE 3: Inferred from homology;
KW Monooxygenase {ECO:0000313|EMBL:EKF21986.1};
KW Oxidoreductase {ECO:0000313|EMBL:VCT92199.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000006265}.
FT DOMAIN 79..338
FT /note="Glutamate synthase"
FT /evidence="ECO:0000259|Pfam:PF01645"
FT DOMAIN 367..410
FT /note="Glutamate synthase"
FT /evidence="ECO:0000259|Pfam:PF01645"
FT REGION 107..127
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..124
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 450 AA; 48143 MW; EE40E53664D01A28 CRC64;
MNNNWGLRES ATFDRYTIAA IQRAAETGIY DIRGWGAKRK VPHFDDLLFL GASMSRYPLE
GYRERCDTDV VLGDRFAKHP LHLDIPITIA GMSFGALSGQ AKEALGRGAS EVGTSTTTGD
GGMTPEERGQ SKYLVYQYLP SRYGMNPDDL RKADAIEVVL GQGAKPGGGG MLLGQKISPR
VAEMRTLPEG IDQRSACRHP DWTGPDDLAI KIQELREITD WEKPIYVKVG ATRTYYDVKL
AVHAGADVVV VDGMQGGTAA TQEVFIEHVG IPTLAAIPQA VQALQELGVH RTQASGADRK
GVQLIVSGGI RTGADVAKAL ALGADAVAIG TAALIALGDN HPRYQAEYEK IGSAAGFFDD
FQDGRDPAGI STQDPELAAR LDPVEGGRRL ANYLRVLTME AQTIARACGK AHVCHLEPED
LVAVTIEAAA MARVPLAGTD WIPGQGNSGW
//
