ID K5W668_PHACS Unreviewed; 3574 AA.
AC K5W668;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 28-JUN-2023, entry version 53.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=PHACADRAFT_174950 {ECO:0000313|EMBL:EKM54444.1};
OS Phanerochaete carnosa (strain HHB-10118-sp) (White-rot fungus) (Peniophora
OS carnosa).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Polyporales; Phanerochaetaceae; Phanerochaete.
OX NCBI_TaxID=650164 {ECO:0000313|EMBL:EKM54444.1, ECO:0000313|Proteomes:UP000008370};
RN [1] {ECO:0000313|EMBL:EKM54444.1, ECO:0000313|Proteomes:UP000008370}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HHB-10118-sp {ECO:0000313|EMBL:EKM54444.1,
RC ECO:0000313|Proteomes:UP000008370};
RX PubMed=22937793; DOI=10.1186/1471-2164-13-444;
RA Suzuki H., MacDonald J., Syed K., Salamov A., Hori C., Aerts A.,
RA Henrissat B., Wiebenga A., vanKuyk P.A., Barry K., Lindquist E.,
RA LaButti K., Lapidus A., Lucas S., Coutinho P., Gong Y., Samejima M.,
RA Mahadevan R., Abou-Zaid M., de Vries R.P., Igarashi K., Yadav J.S.,
RA Grigoriev I.V., Master E.R.;
RT "Comparative genomics of the white-rot fungi, Phanerochaete carnosa and P.
RT chrysosporium, to elucidate the genetic basis of the distinct wood types
RT they colonize.";
RL BMC Genomics 13:444-444(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the UPL family. TOM1/PTR1 subfamily.
CC {ECO:0000256|ARBA:ARBA00034494}.
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DR EMBL; JH930473; EKM54444.1; -; Genomic_DNA.
DR RefSeq; XP_007397136.1; XM_007397074.1.
DR STRING; 650164.K5W668; -.
DR GeneID; 18909697; -.
DR KEGG; pco:PHACADRAFT_174950; -.
DR HOGENOM; CLU_000215_0_0_1; -.
DR InParanoid; K5W668; -.
DR OrthoDB; 164548at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000008370; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd00078; HECTc; 1.
DR CDD; cd14270; UBA; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR010309; E3_Ub_ligase_DUF908.
DR InterPro; IPR010314; E3_Ub_ligase_DUF913.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR025527; HUWE1/Rev1_UBM.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR PANTHER; PTHR11254:SF67; E3 UBIQUITIN-PROTEIN LIGASE HUWE1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF06012; DUF908; 1.
DR Pfam; PF06025; DUF913; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00627; UBA; 1.
DR Pfam; PF14377; UBM; 3.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00165; UBA; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50030; UBA; 1.
PE 3: Inferred from homology;
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000008370};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 1271..1311
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
FT DOMAIN 3239..3574
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 200..246
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 953..1010
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1311..1420
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1738..1760
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1799..1835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2053..2198
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2226..2276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2427..2498
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2578..2597
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2650..2676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2870..2930
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 204..220
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 232..246
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 972..1001
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1312..1365
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1399..1416
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1738..1755
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2067..2085
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2110..2197
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2259..2275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2427..2464
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2472..2491
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2870..2888
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2897..2930
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 3541
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 3574 AA; 395220 MW; B1B7926E4A2D362A CRC64;
MKILHKSRRS VPLIPVVAEL IDKLADTPTE ELSDALAQID HWRWPRSDLN AWVKILNKFD
TIMEDVIRDY EIEKAQLKPF SASDKQLLCE ILRFERLLLE NSTNRKTYNS YDRLNSFMTT
SDLDVLIYSL NLLLRPAQQY SAQPAVSHAL SLNTSRLTSL SKRWPSLHDY DINLVALAGE
QGRAQVDSLP NEAREVAFTF YRKDGSQSKK EEKKEPEVDP FEAPPPQTPR KGASSSPSAT
SANGPVTVHI DSQSLETKPA MQSWAEANET YSVPDDERFE LLCRIRSAKA LARTHAEDRE
KLVIVRLLAI GLFCHTHPEQ TTFNNLFLYE PDLVHHIAEL LQLDRGIDIQ VQTAAVYALD
AVGRYRNKIQ DVLTAVNAGV NHGILMALLR KTITELANAE SSTPQAFVEA LLSFVTYIAA
HAAGGNMVVS AGLIPLLVQV IENRLPNRLY ALSKTMQLLD NILYGYTNAF QLFCNARGID
VLVGRIEYEV DTGLDEHGGG KPAAEIPILY GKISVGRATV LKHIMRSMHR MMQSSGTSEG
LRGLLDSSLV QSVKKVMADR SVFGANVLAI AINIMATFIH NEPTCLPVIQ EAGLPEAFYT
VVESGLEPVI EVVQSIPNAM GALCLNQIGQ DQLASRPGII PGFFSIFTSE KHQRMLQEKE
NAVIIGTAVE ELVRHHPSLK NQVFEAIKQT MAKIEELGNA YQVPEENKHW YVLRATRTTN
ASVGTSDVDV DMDASAQPSS SSIAASAEPE ATPHIFEDSS FKSHDNAIVS FIDVLGKFLE
GYFQHVPHCR DFLSSTDGLD RLARLTALPC IPYDFANSVA SDSLVQVVRT MVEASTNESL
AFIVKIVNES LIATASLRDD NAGNSKYLEL ATVEDEQQAA EANTAFRNLV TLHVRIMLLS
DIFATAGYSQ GRALATLLQS VTGTGTGDIL LGLGSLHRSS IWENVMLKEK MTIRPEDKQQ
TPEAFAPVET PMSVSSEATT PGPSTTANGS QPENSATATP KPEHPTKKDP KEMNMRALRH
LASQLPNSLA PFFQSVVRLF QTRRNPDQAQ KQRILDAADV VADVAVKHLS ARQTDDKRNL
FTYYTMMLGL VTILLIDERT NSKVAHTVLL QAFLRKGGVD AIVTLCREFI ATIDEITPIT
PEERTELQSQ TLIHAHGGLK VALHLIHPLV SFKPIVDSPQ SGFLLATDKK DTEPGWFDPR
NLVVQLRVAI LPLLKDIWQS SWLMSAPLGV SKSVIQVVLE LLNTDQEEIK EVEPAAPSMV
PAIPIATRTI GPDENRIRQL TDMGFPRSAA ERALIRARNN VSHATELLLA QPFPLPPDPE
PEPAAPAPEP ADSAPEPADP APEPIVPAPE PIVPAPEPAA PAPAAPTVED APAQQPAADE
AAATAVIAPA PAPTESTPVP EAEASSSSSS SPPLAPQKSH EEWLKELNDL REPLKESLGS
RILSLVDEHP ALVFDVQKVF VGPSAPYRDQ AVNRLTEDVK KFSSAALDHH EQPLSVRFRI
LALVLNDPSS PLAAMPEAEA TSLMDTLIGL LLSTPINIEN GHVTIPKWLA SHLLVTEAVL
MMGEEPRSVG LPTKEDEPLP EIDAVETGPK FNEKRPVVLD FCLRLLHVPQ LPRDELLSSL
RLFMLLTRDH ALALEFVKRD GISALFRLLR DSPTSASAPG THPYIASILR HIVEDPVTLK
QIMQQEVKAF LSHPRHRHLE AGSFVRTCGA MALRDPKVFV QATAEVCQLS NPYGPLKNLN
LKESHKSNSD GTSGAEGSGE MHIDVSVSQR PSSESLDSLV HFLISELIKS VKVPEQKLSS
EGAATQAPLS GSSTAVQPAD SSTSTSTEIK DSSPTPSIDH TYSCFVMQCL TELLFSYDSC
KVSFLSYSPK KRTHTPAKEK HRTHALQFLI NDLLSFGTIN PSPPSEAKQQ IMLCNWAMSV
IVALCVDTTP TEIKDVPSER ASVRKFVLDA INRAIKDLPG SEMGEARYSR LLALADLCYR
LLTVRFNTGT RKINDDAPTH IAKVMLEKNF VATLTNALAE VDPNFPDIRG VVTGILRPLE
YLTKIAIKMS RASDKQKDSP LDKEESIDSE ISEEDEDEEM EDADREEAPD LYRNSSLGMY
GGEMEDVHFG HDDEMDEDAE DEDDDAEMYD EEETGSEDTS ATDEEDEEAL EQELEDETGD
SEGGWNEAEE EEDLVENEEG HEDADEDSED GEEAGEEAEV MWQPIPTDLN AREGDQMFWH
GMVRSGRRRG EDDGLDIFGR PRTTSTGIPE GTTHPLLLDP NTAAPTSQSR SSRRAHRGTI
AGFPQDLLHS IEHALGEGAI QLFQHIIQGR GGAGETIRID VPANQLIPQL GRHGRAGISA
HIRLERAPRN DGRSDTRGFE PLLTRDRWAE EVKSLHGRFE QSRITKITGH VVLALLPEAI
EAHKKAKLEE EKEAARRREA QQKAEEEAVR KREEEEKVRQ EEERAKAEKE RLEAEARAAE
AESADQSADA DTEMADATTQ GQPSAAEEGQ PEAGSSSVPE RVTVLIHGNE VDITDTGIDP
TFLEALPDDM REEVLNQHIR DQRAARVERP VDSQISPEFL DALPPELRAE IIQQENLERA
RRQAQQPTAA PAQPAVPAEM DPADFLASLD PALRQTVLMD SDEMFIQALP PHMLAEVGLF
RDAQNAARSR AQPAPQLAVR QGGRQPPAGP GKPPASREAI QLLDKHAIAV LIRLLFFPQV
LRKNLLSKVL VNLSENGKTR TDIFNLLLGI LQDGTGDLSS IDRSFAQMSF RNTKSSAQIT
PHTPGKATLP QPEVVPELVA QRCLDALTYI TATNEASSLF FLTEQELPTG LRRSSSKKGK
GKEKQTSQAY YPVVLLLGQL DRQTLLRTPS LMESVAGLLS LVTKPLTSLK DAQKEKEKEK
EKDANPEADR SVTAPLEIQP TAPSGITAQL PQGNSSAPSN IPETTGTQAL PPTADKVLLS
HPPNIPQQVL RHVVNILTAG ECSGRAFSHT LALIQHLSFV SGARDVIAQE LRTRAQEFGQ
RLYSSLDELA TALQESLQEL RTEDVALTVA SKFSPASSDQ AKLLRVLKTI DYMYSPKSIA
SAADATNEDV EKVQSIYESF RFTTLWRRLG DCLSVIEERP ELEHIATVLL PLIESLMVVC
KYVGPKASSS TTSRAVRASA SPRTPTTARE SMEDLFVSFT DAHRKVLNLM VRNNPSLMSG
SFSLLVNNPR VLDFDNKRNY FNQQLHRRLH SREHHGTLQL NVRRQRVFED SFQYLQRKSG
EQIKYGKLSV RFYDEEGVDA GGVTREWFQI LARQMFDPNY CLFQPCAADR LTYQPNKASS
INPEHLSFFK FVGRIIGKAI YDGRLLDAYF ARSLYRQILG KPVDYRDVEW VDPEYYKSLC
WILENDPTLL DLTFSVEADE FGVTKLIELK ENGAHIPVTN ENKREFVQLS ANYRLYSSIK
DQIEALLTGF YEIIPKDLIQ IFDEKELELL ISGTPDIDVD EWRAATEYNG YTSSDPVIVW
FWRALKSFNR EERAKVLSFA TGTSRVPLGG FVDLQGVQGV QRFSIHKAYG EVDRLPQAHT
CFNQIDLPQY SSYEMLRQQL LLAIHEGGEG FGFA
//
