ID K6WXA3_9ACTN Unreviewed; 419 AA.
AC K6WXA3;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Peptidase M20 family protein {ECO:0000313|EMBL:GAB91184.1};
GN ORFNames=GORHZ_125_00670 {ECO:0000313|EMBL:GAB91184.1};
OS Gordonia rhizosphera NBRC 16068.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Gordoniaceae;
OC Gordonia.
OX NCBI_TaxID=1108045 {ECO:0000313|EMBL:GAB91184.1, ECO:0000313|Proteomes:UP000008363};
RN [1] {ECO:0000313|EMBL:GAB91184.1, ECO:0000313|Proteomes:UP000008363}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 16068 {ECO:0000313|EMBL:GAB91184.1,
RC ECO:0000313|Proteomes:UP000008363};
RA Takarada H., Isaki S., Hosoyama A., Tsuchikane K., Katsumata H., Baba S.,
RA Ohji S., Yamazaki S., Fujita N.;
RT "Whole genome shotgun sequence of Gordonia rhizosphera NBRC 16068.";
RL Submitted (AUG-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRSR:PIRSR001235-1};
CC Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000256|PIRSR:PIRSR001235-
CC 1};
CC -!- SIMILARITY: Belongs to the peptidase M20 family.
CC {ECO:0000256|ARBA:ARBA00006153}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAB91184.1}.
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DR EMBL; BAHC01000125; GAB91184.1; -; Genomic_DNA.
DR RefSeq; WP_006334472.1; NZ_BAHC01000125.1.
DR AlphaFoldDB; K6WXA3; -.
DR STRING; 1108045.GORHZ_125_00670; -.
DR eggNOG; COG0624; Bacteria.
DR OrthoDB; 9808195at2; -.
DR Proteomes; UP000008363; Unassembled WGS sequence.
DR GO; GO:0016813; F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.360; -; 1.
DR Gene3D; 3.40.630.10; Zn peptidases; 1.
DR InterPro; IPR010158; Amidase_Cbmase.
DR InterPro; IPR036264; Bact_exopeptidase_dim_dom.
DR InterPro; IPR002933; Peptidase_M20.
DR NCBIfam; TIGR01879; hydantase; 1.
DR PANTHER; PTHR32494:SF5; ALLANTOATE DEIMINASE; 1.
DR PANTHER; PTHR32494; ALLANTOATE DEIMINASE-RELATED; 1.
DR Pfam; PF01546; Peptidase_M20; 1.
DR PIRSF; PIRSF001235; Amidase_carbamoylase; 1.
DR SUPFAM; SSF55031; Bacterial exopeptidase dimerisation domain; 1.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001235-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000008363};
KW Zinc {ECO:0000256|PIRSR:PIRSR001235-1}.
FT BINDING 85
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-1"
FT BINDING 96
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-1"
FT BINDING 96
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-1"
FT BINDING 131
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-1"
FT BINDING 196
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="1"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-1"
FT BINDING 221
FT /ligand="allantoate"
FT /ligand_id="ChEBI:CHEBI:17536"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-2"
FT BINDING 280
FT /ligand="allantoate"
FT /ligand_id="ChEBI:CHEBI:17536"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-2"
FT BINDING 293
FT /ligand="allantoate"
FT /ligand_id="ChEBI:CHEBI:17536"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-2"
FT BINDING 383
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_label="2"
FT /evidence="ECO:0000256|PIRSR:PIRSR001235-1"
SQ SEQUENCE 419 AA; 43828 MW; 91731820D17E32EE CRC64;
MTGTTTTAQS VSTLMAEIAA IGTDAARGGY SRPVYSPAEL ELREWFIAAA RRRSLEIETD
RNGIIWAWWN PAELPLTDAV VTGSHLDSVP GGGAFDGPLG VASALSAIDI LAAQDLAPTR
PLALAVFPEE EGSRYGLACL GSQLLTGAVT PERAAALVDA DGTSFAELAA RNGLDPRHLG
RDDERLSRLG CFVELHVEQG RGLVDLDTPV AIGSSILGHG RWRLSFLGQG NHAGTTLMTD
RRDPMLAAAA TVLEVRRLGR TVDGARATVG KLDPVPGGSN VIASRVDVWL DVRHDDDAVV
AALVTDIAAA ARERAAAEGC SVTLTEASMS PRVDFDPVLR GRLSTILPDA PILSTGAGHD
AGVLKEEIPT AMLFVRNPTG VSHSPEEYVE DPDADAGGHA LATVLADLIT QPASPDSIP
//
