ID K7FYM3_PELSI Unreviewed; 499 AA.
AC K7FYM3;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE SubName: Full=5'-nucleotidase domain containing 3 {ECO:0000313|Ensembl:ENSPSIP00000013133.1};
GN Name=NT5DC3 {ECO:0000313|Ensembl:ENSPSIP00000013133.1};
OS Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Trionychia;
OC Trionychidae; Pelodiscus.
OX NCBI_TaxID=13735 {ECO:0000313|Ensembl:ENSPSIP00000013133.1, ECO:0000313|Proteomes:UP000007267};
RN [1] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RG Soft-shell Turtle Genome Consortium;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RX PubMed=23624526; DOI=10.1038/ng.2615;
RA Wang Z., Pascual-Anaya J., Zadissa A., Li W., Niimura Y., Huang Z., Li C.,
RA White S., Xiong Z., Fang D., Wang B., Ming Y., Chen Y., Zheng Y.,
RA Kuraku S., Pignatelli M., Herrero J., Beal K., Nozawa M., Li Q., Wang J.,
RA Zhang H., Yu L., Shigenobu S., Wang J., Liu J., Flicek P., Searle S.,
RA Wang J., Kuratani S., Yin Y., Aken B., Zhang G., Irie N.;
RT "The draft genomes of soft-shell turtle and green sea turtle yield insights
RT into the development and evolution of the turtle-specific body plan.";
RL Nat. Genet. 45:701-706(2013).
RN [3] {ECO:0000313|Ensembl:ENSPSIP00000013133.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR017434-2};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR017434-2};
CC -!- SIMILARITY: Belongs to the 5'(3')-deoxyribonucleotidase family.
CC {ECO:0000256|ARBA:ARBA00009589}.
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DR EMBL; AGCU01195383; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01195384; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; K7FYM3; -.
DR Ensembl; ENSPSIT00000013196.1; ENSPSIP00000013133.1; ENSPSIG00000011749.1.
DR eggNOG; KOG2470; Eukaryota.
DR GeneTree; ENSGT00940000158637; -.
DR HOGENOM; CLU_017845_5_1_1; -.
DR OMA; WSRYKEA; -.
DR TreeFam; TF323990; -.
DR Proteomes; UP000007267; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0043235; C:receptor complex; IEA:Ensembl.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd07522; HAD_cN-II; 1.
DR Gene3D; 3.40.50.1000; HAD superfamily/HAD-like; 1.
DR InterPro; IPR036412; HAD-like_sf.
DR InterPro; IPR008380; HAD-SF_hydro_IG_5-nucl.
DR InterPro; IPR023214; HAD_sf.
DR InterPro; IPR016695; Pur_nucleotidase.
DR NCBIfam; TIGR02244; HAD-IG-Ncltidse; 1.
DR PANTHER; PTHR12103; 5'-NUCLEOTIDASE DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR12103:SF11; 5'-NUCLEOTIDASE DOMAIN-CONTAINING PROTEIN 3; 1.
DR Pfam; PF05761; 5_nucleotid; 1.
DR PIRSF; PIRSF017434; Purine_5'-nucleotidase; 1.
DR SUPFAM; SSF56784; HAD-like; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842, ECO:0000256|PIRSR:PIRSR017434-2};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR017434-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000007267}.
FT ACT_SITE 50
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT ACT_SITE 52
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-1"
FT BINDING 50
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 52
FT /ligand="GMP"
FT /ligand_id="ChEBI:CHEBI:58115"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
FT BINDING 337
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR017434-2"
SQ SEQUENCE 499 AA; 59136 MW; 370DA78658C8DCCC CRC64;
MKRYLWARYK EAKKVTKELV PSIMNNLLNP DAIFSNNEMS LSDIEIYGFD YDYTLVFYSK
HLHTLIYNAA RDLLINEHRY PAEIRKYDYD PNFAIRGLHY DVHRALLMKI DAFHYIQLGT
VYRGLSVVPD EEVIAMYDGS HVPLEQMSDF YGKSSHGSTM KQFMDIFSLP EMTLLSCVNE
YFLKNNIDYE PVHLYKDVKD SIRDVHIKGI MYQAIEADIE KYICYAEQTR AVLGKLADHG
KKMFLITNSP SSFVDKGMKF IVGKDWRDLF DVVIVQADKP NFFNDKRRPF RKVNERGVLL
WDKIHKLQKG QIYKQGNLYE FLKLTGWRGS KVLYFGDHIY SDLADLTLKH GWRTGAIIPE
LRSEIKIMNT EQYIQTMTWL QTLTGLLEHM QVHRDPESQM ILQEWKKERK EMREMTRNFF
NSQFGSLFRT DQNPTYFLRR LSRFADIYMA SLSCLLNYDP DYTFYPRRTP LQHELPTWSD
QLCTGTFRIP FLQEMVQIK
//
