ID K7G385_PELSI Unreviewed; 570 AA.
AC K7G385;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=Hyaluronidase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
DE EC=3.2.1.35 {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
GN Name=SPAM1 {ECO:0000313|Ensembl:ENSPSIP00000014746.1};
OS Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Trionychia;
OC Trionychidae; Pelodiscus.
OX NCBI_TaxID=13735 {ECO:0000313|Ensembl:ENSPSIP00000014746.1, ECO:0000313|Proteomes:UP000007267};
RN [1] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RG Soft-shell Turtle Genome Consortium;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RX PubMed=23624526; DOI=10.1038/ng.2615;
RA Wang Z., Pascual-Anaya J., Zadissa A., Li W., Niimura Y., Huang Z., Li C.,
RA White S., Xiong Z., Fang D., Wang B., Ming Y., Chen Y., Zheng Y.,
RA Kuraku S., Pignatelli M., Herrero J., Beal K., Nozawa M., Li Q., Wang J.,
RA Zhang H., Yu L., Shigenobu S., Wang J., Liu J., Flicek P., Searle S.,
RA Wang J., Kuratani S., Yin Y., Aken B., Zhang G., Irie N.;
RT "The draft genomes of soft-shell turtle and green sea turtle yield insights
RT into the development and evolution of the turtle-specific body plan.";
RL Nat. Genet. 45:701-706(2013).
RN [3] {ECO:0000313|Ensembl:ENSPSIP00000014746.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-
CC glucosamine and D-glucuronate residues in hyaluronate.; EC=3.2.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00000251,
CC ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004609};
CC Lipid-anchor, GPI-anchor {ECO:0000256|ARBA:ARBA00004609}. Membrane
CC {ECO:0000256|ARBA:ARBA00004589}; Lipid-anchor, GPI-anchor
CC {ECO:0000256|ARBA:ARBA00004589}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 56 family.
CC {ECO:0000256|ARBA:ARBA00008871, ECO:0000256|PIRNR:PIRNR038193,
CC ECO:0000256|RuleBase:RU610713}.
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DR EMBL; AGCU01083595; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_006122658.1; XM_006122596.2.
DR RefSeq; XP_006122659.1; XM_006122597.2.
DR RefSeq; XP_006122661.1; XM_006122599.2.
DR RefSeq; XP_014428871.1; XM_014573385.1.
DR AlphaFoldDB; K7G385; -.
DR STRING; 13735.ENSPSIP00000014746; -.
DR GlyCosmos; K7G385; 1 site, No reported glycans.
DR Ensembl; ENSPSIT00000014816.1; ENSPSIP00000014746.1; ENSPSIG00000013181.1.
DR GeneID; 102458764; -.
DR KEGG; pss:102458764; -.
DR eggNOG; ENOG502R6HD; Eukaryota.
DR GeneTree; ENSGT01020000230364; -.
DR OMA; RAKIVFE; -.
DR OrthoDB; 5344684at2759; -.
DR TreeFam; TF321598; -.
DR Proteomes; UP000007267; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0098552; C:side of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004415; F:hyalurononglucosaminidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0007342; P:fusion of sperm to egg plasma membrane involved in single fertilization; IEA:InterPro.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR018155; Hyaluronidase.
DR InterPro; IPR001439; Hyaluronidase_PH20/Hyal5.
DR PANTHER; PTHR11769; HYALURONIDASE; 1.
DR PANTHER; PTHR11769:SF20; HYALURONIDASE PH-20; 1.
DR Pfam; PF01630; Glyco_hydro_56; 1.
DR PIRSF; PIRSF038193; Hyaluronidase; 1.
DR PIRSF; PIRSF500773; Hyaluronidase_PH20_Hyal5; 1.
DR PRINTS; PR00846; GLHYDRLASE56.
DR PRINTS; PR00848; SPERMPH20.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE 3: Inferred from homology;
KW Disulfide bond {ECO:0000256|PIRSR:PIRSR038193-3};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00022622};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR038193};
KW GPI-anchor {ECO:0000256|ARBA:ARBA00022622};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR038193, ECO:0000256|RuleBase:RU610713};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00022622};
KW Reference proteome {ECO:0000313|Proteomes:UP000007267}.
FT ACT_SITE 148
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-1"
FT CARBOHYD 371
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-2"
FT DISULFID 59..354
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 224..240
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 379..390
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 384..438
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
FT DISULFID 440..446
FT /evidence="ECO:0000256|PIRSR:PIRSR038193-3"
SQ SEQUENCE 570 AA; 64546 MW; CE6C514986132226 CRC64;
MEILKQAQSF GSFIASNRAS WLVFIIIQVT CCSSLHIRAQ PLIPNSPFLS IWNAPTELCT
EKTKVQLDMR LFHLVGSTLK TSTEQNITIF YADRLGNYPY IDENTHKTFN GGIPQLCFLE
HHLKKARDDI NFYMPSDEQV GLAVIDWENW RPLWIRNWGA KDIYRQESVE FVQQNDISLS
ETQVRTLAKM EFEAAAKSFM LHSLKLGISM KPNNLWGYYL YPDCYNYDYN QNPHNYTGSC
LDIEIARNNE LNWLWEESTA LYPSVYLETA LRSSKNAPLF VRNRVQEAIR VSNVSNSTCP
LPIFVYTRPV FTDFNQEYLS QDDLVNTIGE SAALGVSGIV IWGDLHLALS VNTCRTLGSY
LGHTLNPYII NVTMAAKICS QALCQDSGAC ARKNWNSSDY LHLNPENFII QMSKDGKYAV
QGQPSFQDLQ QFIEKFYCRC YAGYSCKSRA DVNDIHYINA CISEDICVQV SLNSLSNMDA
SGARASSNMS VFSLTPEKNM TLPALNATEG FQQTIRNNKF TVSSAEHTSA TTNSKVDLKI
TDTHSFSDSS AEEMKILNMA CLILILRNLI
//
