ID K7GAU8_PELSI Unreviewed; 1047 AA.
AC K7GAU8;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=sn-1-specific diacylglycerol lipase {ECO:0000256|ARBA:ARBA00026104};
DE EC=3.1.1.116 {ECO:0000256|ARBA:ARBA00026104};
GN Name=DAGLA {ECO:0000313|Ensembl:ENSPSIP00000017409.1};
OS Pelodiscus sinensis (Chinese softshell turtle) (Trionyx sinensis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Trionychia;
OC Trionychidae; Pelodiscus.
OX NCBI_TaxID=13735 {ECO:0000313|Ensembl:ENSPSIP00000017409.1, ECO:0000313|Proteomes:UP000007267};
RN [1] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RG Soft-shell Turtle Genome Consortium;
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000007267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Daiwa-1 {ECO:0000313|Proteomes:UP000007267};
RX PubMed=23624526; DOI=10.1038/ng.2615;
RA Wang Z., Pascual-Anaya J., Zadissa A., Li W., Niimura Y., Huang Z., Li C.,
RA White S., Xiong Z., Fang D., Wang B., Ming Y., Chen Y., Zheng Y.,
RA Kuraku S., Pignatelli M., Herrero J., Beal K., Nozawa M., Li Q., Wang J.,
RA Zhang H., Yu L., Shigenobu S., Wang J., Liu J., Flicek P., Searle S.,
RA Wang J., Kuratani S., Yin Y., Aken B., Zhang G., Irie N.;
RT "The draft genomes of soft-shell turtle and green sea turtle yield insights
RT into the development and evolution of the turtle-specific body plan.";
RL Nat. Genet. 45:701-706(2013).
RN [3] {ECO:0000313|Ensembl:ENSPSIP00000017409.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + H2O = a 2-acylglycerol + a fatty
CC acid + H(+); Xref=Rhea:RHEA:33275, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17389, ChEBI:CHEBI:17815,
CC ChEBI:CHEBI:28868; EC=3.1.1.116;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33276;
CC Evidence={ECO:0000256|ARBA:ARBA00024531};
CC -!- COFACTOR:
CC Name=Ca(2+); Xref=ChEBI:CHEBI:29108;
CC Evidence={ECO:0000256|ARBA:ARBA00001913};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004651};
CC Multi-pass membrane protein {ECO:0000256|ARBA:ARBA00004651}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily. Lipase family.
CC {ECO:0000256|ARBA:ARBA00010701}.
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DR EMBL; AGCU01001054; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001055; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001056; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001057; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001058; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001059; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001060; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001061; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001062; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AGCU01001063; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR STRING; 13735.ENSPSIP00000017409; -.
DR Ensembl; ENSPSIT00000017488.1; ENSPSIP00000017409.1; ENSPSIG00000015323.1.
DR eggNOG; KOG2088; Eukaryota.
DR GeneTree; ENSGT00940000161192; -.
DR TreeFam; TF312928; -.
DR Proteomes; UP000007267; Unassembled WGS sequence.
DR GO; GO:0032591; C:dendritic spine membrane; IEA:Ensembl.
DR GO; GO:0031901; C:early endosome membrane; IEA:Ensembl.
DR GO; GO:0098839; C:postsynaptic density membrane; IEA:Ensembl.
DR GO; GO:0043196; C:varicosity; IEA:Ensembl.
DR GO; GO:0047372; F:acylglycerol lipase activity; IEA:Ensembl.
DR GO; GO:0019369; P:arachidonic acid metabolic process; IEA:Ensembl.
DR GO; GO:1901696; P:cannabinoid biosynthetic process; IEA:Ensembl.
DR GO; GO:0046340; P:diacylglycerol catabolic process; IEA:Ensembl.
DR GO; GO:0007216; P:G protein-coupled glutamate receptor signaling pathway; IEA:Ensembl.
DR GO; GO:0006640; P:monoacylglycerol biosynthetic process; IEA:Ensembl.
DR GO; GO:0007405; P:neuroblast proliferation; IEA:Ensembl.
DR GO; GO:0150077; P:regulation of neuroinflammatory response; IEA:Ensembl.
DR GO; GO:0098921; P:retrograde trans-synaptic signaling by endocannabinoid; IEA:Ensembl.
DR CDD; cd00519; Lipase_3; 1.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR002921; Fungal_lipase-like.
DR PANTHER; PTHR45792; DIACYLGLYCEROL LIPASE HOMOLOG-RELATED; 1.
DR PANTHER; PTHR45792:SF8; DIACYLGLYCEROL LIPASE-ALPHA; 1.
DR Pfam; PF01764; Lipase_3; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000007267};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 18..41
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 102..123
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 135..157
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 292..310
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 395..530
FT /note="Fungal lipase-like"
FT /evidence="ECO:0000259|Pfam:PF01764"
FT REGION 827..891
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 827..865
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 866..880
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1047 AA; 116728 MW; C253DC06AE97877B CRC64;
MPGIVVFRRR WSVGSDDLVL PAIFLFLLHT TWFVILSVVL FGLAYNPNET CSLNLVDHGR
GYLGILLSCM IAEVAIIWLS MRGSILYTEP RDSMQYVLYV RLAILVIEFV YAIVGIVWLT
QYYSSCNDLT AKSVTLGMVV CNWVVILSVC ITVLCVFDPT GRTFVKLRAT KRRQRNLRTY
NLRHRLEEGQ ASSWTRRLKV FLCCTRTKDS QSDAYSEIAY LFAEFFRDLD IVPSDIIAGL
VLLRQRQRAK RNAVLDEANN DILAFLSGMP VTRNTKYLDL KNSQEMLRYK EVCYYMLFAL
AAYGWPMYLM RKPTCGLCRL ARSCSCCCLC SSRPRYAPSV TIEEDNCCGC NAIAIRRHFL
DENMTSVDIV YTSCHDAVYE TPFYVAVDHD KKKVVISIRG TLSPKDALTD LTGDAERLPV
EGHHGTWLGH KGMVLSAEYI KKKLEQEMVL SQAFGRDLGR GTKHYGLIVV GHSLGAGTAA
ILSFMLRPQY PSLKCYAYSP PGGLLSEDAM EYSKEFVTAV VLGKDLVPRI GLSQLEGFRR
QLLDVLQRSN KPKWRIIVGA TKCIPKSELP EETEGNSVTS NRLWTHPSDL TIALSASTPL
YPPGRIIHVV HNHPAEQCCC CEQEDPTYFA IWGDNKAFNE VIISPAMLHE HLPYVVMEGL
NKVLENYNKG KTALLSAAKV MVSPTEVDLN PELIFQNQPL PSGPSVQIGI GAITVDRRNS
SVKSKSHSEI SLEGFYETKL LSPVQKDPVE LLLLDTKERL SVELQDRRAP LATMESLSDN
ESIYSFESRR SSGFRSIRGS PSLHVVMEKD ETHCFYIDPV IPEENPSLSS RTELLAADSL
SKHSQETQPP EGLANSGGTT PQRRGSEEGA RSEGDHVSFT PREELSLQNG RLTDVPSPQV
LEFAEFIDSL FNLDSKSSSF QDLYCMMVSD SSSDFAEMPK SVSDQEILLR AQYEPNLVPK
PPRLFAGSAD PSSGISVSPS FPLSSSAELM DITPTGMSSQ ECXXXXXXXX XXXXXXXXXX
XXXXXXXXXA GHVTSPAKQE DLMISAL
//
