ID K7KU45_SOYBN Unreviewed; 636 AA.
AC K7KU45;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 62.
DE RecName: Full=rhamnogalacturonan endolyase {ECO:0000256|ARBA:ARBA00012437};
DE EC=4.2.2.23 {ECO:0000256|ARBA:ARBA00012437};
GN Name=100814185 {ECO:0000313|EnsemblPlants:KRH52883};
GN ORFNames=GLYMA_06G092800 {ECO:0000313|EMBL:KRH52884.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EMBL:KRH52884.1};
RN [1] {ECO:0000313|EMBL:KRH52884.1, ECO:0000313|EnsemblPlants:KRH52883}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRH52883};
RC TISSUE=Callus {ECO:0000313|EMBL:KRH52884.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRH52883}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRH52883};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRH52884.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRH52884.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endotype eliminative cleavage of L-alpha-rhamnopyranosyl-
CC (1->4)-alpha-D-galactopyranosyluronic acid bonds of
CC rhamnogalacturonan I domains in ramified hairy regions of pectin
CC leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-
CC unsaturated D-galactopyranosyluronic acid at the non-reducing end.;
CC EC=4.2.2.23; Evidence={ECO:0000256|ARBA:ARBA00001324};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the polysaccharide lyase 4 family.
CC {ECO:0000256|ARBA:ARBA00010418}.
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DR EMBL; CM000839; KRH52883.1; -; Genomic_DNA.
DR EMBL; CM000839; KRH52884.1; -; Genomic_DNA.
DR RefSeq; XP_003526528.1; XM_003526480.2.
DR RefSeq; XP_006581481.1; XM_006581418.2.
DR AlphaFoldDB; K7KU45; -.
DR STRING; 3847.K7KU45; -.
DR PaxDb; 3847-GLYMA06G09760-2; -.
DR EnsemblPlants; KRH52883; KRH52883; GLYMA_06G092800.
DR EnsemblPlants; KRH52884; KRH52884; GLYMA_06G092800.
DR GeneID; 100814185; -.
DR Gramene; KRH52883; KRH52883; GLYMA_06G092800.
DR Gramene; KRH52884; KRH52884; GLYMA_06G092800.
DR KEGG; gmx:100814185; -.
DR eggNOG; ENOG502QQM5; Eukaryota.
DR HOGENOM; CLU_015099_1_0_1; -.
DR InParanoid; K7KU45; -.
DR OMA; WHGFMWD; -.
DR OrthoDB; 1215403at2759; -.
DR Proteomes; UP000008827; Chromosome 6.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0102210; F:rhamnogalacturonan endolyase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR CDD; cd10317; RGL4_C; 1.
DR CDD; cd10316; RGL4_M; 1.
DR CDD; cd10320; RGL4_N; 1.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.40.1120; Carboxypeptidase-like, regulatory domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR013784; Carb-bd-like_fold.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR029413; RG-lyase_II.
DR InterPro; IPR029411; RG-lyase_III.
DR InterPro; IPR010325; Rhamnogal_lyase.
DR PANTHER; PTHR32018:SF8; RHAMNOGALACTURONAN ENDOLYASE; 1.
DR PANTHER; PTHR32018; RHAMNOGALACTURONATE LYASE FAMILY PROTEIN; 1.
DR Pfam; PF14683; CBM-like; 1.
DR Pfam; PF14686; fn3_3; 1.
DR Pfam; PF06045; Rhamnogal_lyase; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF49452; Starch-binding domain-like; 1.
PE 3: Inferred from homology;
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}.
FT DOMAIN 354..426
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14686"
FT DOMAIN 440..628
FT /note="Rhamnogalacturonan lyase"
FT /evidence="ECO:0000259|Pfam:PF14683"
SQ SEQUENCE 636 AA; 72437 MW; B2AE97FB3BCF3678 CRC64;
MSGPGVQLHI QDHHVVMDNG IVQVTLSNPD GIVTGIRYNG VDNLLEVLNK ETNRGYWDLV
WSAPGSKGMF DVIKGTCFKV IVQNEEQVEL SFARMWDHSL EGKFVPLNID KRFIMLRGSS
GFYSYGIYEH LNGWPDFDIS ETRITFKLRK DKFQYMAMAD NRQRVMPFPE DRLAGRCQTL
GYPEAVLLVN PKDPRLKGEV DDKYQYSCVN MNNRVHGWIS FSPPVGFWQI TPSDEFRSGG
PLKQNLTSHV GPTTLAMFLS SHYAGQDLVP KFRGGESWKK VFGPVYIYLN SGAVGDNPLW
LWEDAKIQMM NEVQSWPYSF PASEDFLKSD QRGNVSGRLL VLDRYVCTDL IPANSAYVGL
APPGDAGSWQ RECKDYQFWT RADENGFFTI KNVRPGDYNL FAWVPGFVGD YKFGDFMKIT
SGSYIELGEL VYEPPRDGPT LWEIGIPDRS AAEFYAPDPS PQHINKLFIN HPDRFRQYGL
WDRYSELYPD ADLVYTIGVS DYTKDWFYAQ APRKKVDNTL QGTTWQIKFE ICSVVKGSTY
KLRVAIASAT LAELQIRVND PNARRPVFTS GLIGRDNSIA RHGIQGIYWL YHVNIPGSLL
VDGTNTIYFS QPRCTSPFQG IMYDYIRLEG PPCEEI
//
