ID K7MRU1_SOYBN Unreviewed; 616 AA.
AC K7MRU1;
DT 09-JAN-2013, integrated into UniProtKB/TrEMBL.
DT 09-JAN-2013, sequence version 1.
DT 27-MAR-2024, entry version 77.
DE RecName: Full=Purple acid phosphatase {ECO:0000256|RuleBase:RU361203};
DE EC=3.1.3.2 {ECO:0000256|RuleBase:RU361203};
GN Name=100817130 {ECO:0000313|EnsemblPlants:KRG99244};
GN ORFNames=GLYMA_18G132500 {ECO:0000313|EMBL:KRG99244.1};
OS Glycine max (Soybean) (Glycine hispida).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine;
OC Glycine subgen. Soja.
OX NCBI_TaxID=3847 {ECO:0000313|EMBL:KRG99244.1};
RN [1] {ECO:0000313|EMBL:KRG99244.1, ECO:0000313|EnsemblPlants:KRG99244}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Williams 82 {ECO:0000313|EnsemblPlants:KRG99244};
RC TISSUE=Callus {ECO:0000313|EMBL:KRG99244.1};
RX PubMed=20075913; DOI=10.1038/nature08670;
RA Schmutz J., Cannon S.B., Schlueter J., Ma J., Mitros T., Nelson W.,
RA Hyten D.L., Song Q., Thelen J.J., Cheng J., Xu D., Hellsten U., May G.D.,
RA Yu Y., Sakurai T., Umezawa T., Bhattacharyya M.K., Sandhu D.,
RA Valliyodan B., Lindquist E., Peto M., Grant D., Shu S., Goodstein D.,
RA Barry K., Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N.,
RA Joshi T., Libault M., Sethuraman A., Zhang X.-C., Shinozaki K.,
RA Nguyen H.T., Wing R.A., Cregan P., Specht J., Grimwood J., Rokhsar D.,
RA Stacey G., Shoemaker R.C., Jackson S.A.;
RT "Genome sequence of the palaeopolyploid soybean.";
RL Nature 463:178-183(2010).
RN [2] {ECO:0000313|EnsemblPlants:KRG99244}
RP IDENTIFICATION.
RC STRAIN=Williams 82 {ECO:0000313|EnsemblPlants:KRG99244};
RG EnsemblPlants;
RL Submitted (FEB-2018) to UniProtKB.
RN [3] {ECO:0000313|EMBL:KRG99244.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Callus {ECO:0000313|EMBL:KRG99244.1};
RA Schmutz J., Cannon S., Schlueter J., Ma J., Mitros T., Nelson W., Hyten D.,
RA Song Q., Thelen J., Cheng J., Xu D., Hellsten U., May G., Yu Y.,
RA Sakurai T., Umezawa T., Bhattacharyya M., Sandhu D., Valliyodan B.,
RA Lindquist E., Peto M., Grant D., Shu S., Goodstein D., Barry K.,
RA Futrell-Griggs M., Abernathy B., Du J., Tian Z., Zhu L., Gill N., Joshi T.,
RA Libault M., Sethuraman A., Zhang X., Shinozaki K., Nguyen H., Wing R.,
RA Cregan P., Specht J., Grimwood J., Rokhsar D., Stacey G., Shoemaker R.,
RA Jackson S.;
RT "WGS assembly of Glycine max.";
RL Submitted (JUL-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC Evidence={ECO:0000256|RuleBase:RU361203};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple
CC acid phosphatase family. {ECO:0000256|ARBA:ARBA00008723,
CC ECO:0000256|RuleBase:RU361203}.
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DR EMBL; CM000851; KRG99244.1; -; Genomic_DNA.
DR RefSeq; XP_003552004.2; XM_003551956.3.
DR RefSeq; XP_006602367.1; XM_006602304.2.
DR RefSeq; XP_006602368.1; XM_006602305.2.
DR RefSeq; XP_006602369.1; XM_006602306.2.
DR RefSeq; XP_006602370.1; XM_006602307.2.
DR RefSeq; XP_014626553.1; XM_014771067.1.
DR RefSeq; XP_014626554.1; XM_014771068.1.
DR RefSeq; XP_014626555.1; XM_014771069.1.
DR RefSeq; XP_014626556.1; XM_014771070.1.
DR AlphaFoldDB; K7MRU1; -.
DR SMR; K7MRU1; -.
DR STRING; 3847.K7MRU1; -.
DR PaxDb; 3847-GLYMA18G17541-2; -.
DR EnsemblPlants; KRG99244; KRG99244; GLYMA_18G132500.
DR GeneID; 100817130; -.
DR Gramene; KRG99244; KRG99244; GLYMA_18G132500.
DR KEGG; gmx:100817130; -.
DR eggNOG; KOG1378; Eukaryota.
DR HOGENOM; CLU_013387_4_1_1; -.
DR InParanoid; K7MRU1; -.
DR OMA; EICQPEN; -.
DR OrthoDB; 456532at2759; -.
DR Proteomes; UP000008827; Chromosome 18.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0003993; F:acid phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd00839; MPP_PAPs; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR Gene3D; 2.60.40.380; Purple acid phosphatase-like, N-terminal; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR040974; Fn3_PAP.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041792; MPP_PAP.
DR InterPro; IPR008963; Purple_acid_Pase-like_N.
DR InterPro; IPR015914; Purple_acid_Pase_N.
DR InterPro; IPR025733; Purple_acid_PPase_C_dom.
DR PANTHER; PTHR45778:SF34; PURPLE ACID PHOSPHATASE; 1.
DR PANTHER; PTHR45778; PURPLE ACID PHOSPHATASE-RELATED; 1.
DR Pfam; PF17808; fn3_PAP; 1.
DR Pfam; PF00149; Metallophos; 1.
DR Pfam; PF14008; Metallophos_C; 1.
DR Pfam; PF16656; Pur_ac_phosph_N; 1.
DR SUPFAM; SSF56300; Metallo-dependent phosphatases; 1.
DR SUPFAM; SSF49363; Purple acid phosphatase, N-terminal domain; 1.
PE 3: Inferred from homology;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|RuleBase:RU361203};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Reference proteome {ECO:0000313|Proteomes:UP000008827};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU361203};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT SIGNAL 1..27
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT CHAIN 28..616
FT /note="Purple acid phosphatase"
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT /id="PRO_5014486328"
FT DOMAIN 51..169
FT /note="Purple acid phosphatase Fn3-like"
FT /evidence="ECO:0000259|Pfam:PF17808"
FT DOMAIN 175..280
FT /note="Purple acid phosphatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16656"
FT DOMAIN 292..507
FT /note="Calcineurin-like phosphoesterase"
FT /evidence="ECO:0000259|Pfam:PF00149"
FT DOMAIN 532..591
FT /note="Iron/zinc purple acid phosphatase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14008"
SQ SEQUENCE 616 AA; 69376 MW; 37F58151AC522976 CRC64;
MMTNGMGKSR VLFFSMLVLA TFQKAVSEEH QPLSKVAIHK TTLALDERAF IKATPNVLGL
KGQNTEWVTL QYSNPKPTVD DWIGVFSPAN FNASTCPAEN IWVNPPFLCS APIKYQYANF
SSHGYKNTGK GSLKLQLINQ RSDFSFALFT GGLTNPKLVA VSNKVSFINP NAPVYPRLAQ
GKTWDEMTVT WTSGYEISDA EPFVEWGPKG GNLVKSPAGT LTFDRNTMCG APARTVGWRD
PGYIHTSFLK ELWPNREYKY KLGHKLFNGT IIWSQEYQFK ASPYPGQNSL QRVVIFGDMG
KAEADGSNEY NNFQPGSLNT TKQIIQDLKD IDIVFNIGDL SYANGYLSQW DQFTAQIEPI
ASTVPYMTAS GNHERDWPDT GSFYGNLDSG GECGVLAQTM FYVPAENREK FWYSVDYGMF
RFCIANTELD WRKGSEQYKF IENCLASVDR QKQPWLIFLA HRVLGYSSAG FYVAEGSFEE
PMGREDLQYL WQKYKVDIAM YGHVHNYERT CPVYQNICTN KEEHNYKGSL DGTIHVVVGG
GGASLAEFAP INTTWSIFKD HDFGFVKLTA FDHSNLLFEY KKSSDGQVYD SFKISRQYRD
ILACTVDSCP PTTLAS
//
