ID K7UN70_MAIZE Unreviewed; 625 AA.
AC K7UN70;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 24-JAN-2024, entry version 71.
DE RecName: Full=Endoglucanase {ECO:0000256|RuleBase:RU361166};
DE EC=3.2.1.4 {ECO:0000256|RuleBase:RU361166};
GN Name=Cel4 {ECO:0000313|EMBL:AGG19092.1};
GN Synonyms=103642193 {ECO:0000313|EnsemblPlants:Zm00001eb433630_P001};
GN ORFNames=ZEAMMB73_Zm00001d026605 {ECO:0000313|EMBL:AQK47216.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577 {ECO:0000313|EMBL:AQK47216.1};
RN [1] {ECO:0000313|EnsemblPlants:Zm00001eb433630_P001, ECO:0000313|Proteomes:UP000007305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73 {ECO:0000313|EnsemblPlants:Zm00001eb433630_P001,
RC ECO:0000313|Proteomes:UP000007305};
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [2] {ECO:0000313|EMBL:AGG19092.1}
RP NUCLEOTIDE SEQUENCE.
RA Buchanan M., Burton R.A., Dhugga K.S., Rafalski J.A., Tingey S.V.,
RA Shirley N.J., Fincher G.B.;
RT "Endo-(1,4)-beta-Glucanase Gene Families in the Grasses: Temporal and
RT Spatial Co-transcription of Orthologous Genes.";
RL Submitted (NOV-2012) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:AQK47216.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Seedling {ECO:0000313|EMBL:AQK47216.1};
RG Maize Genome Sequencing Project;
RA Ware D.;
RT "Update maize B73 reference genome by single molecule sequencing
RT technologies.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EnsemblPlants:Zm00001eb433630_P001}
RP IDENTIFICATION.
RC STRAIN=cv. B73 {ECO:0000313|EnsemblPlants:Zm00001eb433630_P001};
RG EnsemblPlants;
RL Submitted (MAY-2021) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endohydrolysis of (1->4)-beta-D-glucosidic linkages in
CC cellulose, lichenin and cereal beta-D-glucans.; EC=3.2.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00000966,
CC ECO:0000256|RuleBase:RU361166};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 9 (cellulase E) family.
CC {ECO:0000256|ARBA:ARBA00007072, ECO:0000256|PROSITE-ProRule:PRU10059,
CC ECO:0000256|RuleBase:RU361166}.
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DR EMBL; KC196752; AGG19092.1; -; mRNA.
DR EMBL; CM000786; AQK47216.1; -; Genomic_DNA.
DR RefSeq; NP_001288435.1; NM_001301506.1.
DR AlphaFoldDB; K7UN70; -.
DR SMR; K7UN70; -.
DR STRING; 4577.K7UN70; -.
DR PaxDb; 4577-GRMZM2G343144_P02; -.
DR EnsemblPlants; Zm00001eb433630_T001; Zm00001eb433630_P001; Zm00001eb433630.
DR GeneID; 103642193; -.
DR Gramene; Zm00001eb433630_T001; Zm00001eb433630_P001; Zm00001eb433630.
DR KEGG; zma:103642193; -.
DR eggNOG; ENOG502QRF6; Eukaryota.
DR HOGENOM; CLU_008926_1_4_1; -.
DR InParanoid; K7UN70; -.
DR OMA; GFDKYYN; -.
DR OrthoDB; 1347382at2759; -.
DR Proteomes; UP000007305; Chromosome 10.
DR ExpressionAtlas; K7UN70; baseline.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0008810; F:cellulase activity; IEA:UniProtKB-EC.
DR GO; GO:0030245; P:cellulose catabolic process; IEA:UniProtKB-KW.
DR Gene3D; 1.50.10.10; -; 1.
DR InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR InterPro; IPR019028; CBM_49.
DR InterPro; IPR001701; Glyco_hydro_9.
DR InterPro; IPR033126; Glyco_hydro_9_Asp/Glu_AS.
DR InterPro; IPR018221; Glyco_hydro_9_His_AS.
DR PANTHER; PTHR22298; ENDO-1,4-BETA-GLUCANASE; 1.
DR PANTHER; PTHR22298:SF41; ENDOGLUCANASE 5; 1.
DR Pfam; PF09478; CBM49; 1.
DR Pfam; PF00759; Glyco_hydro_9; 1.
DR SMART; SM01063; CBM49; 1.
DR SUPFAM; SSF48208; Six-hairpin glycosidases; 1.
DR PROSITE; PS00592; GH9_2; 1.
DR PROSITE; PS00698; GH9_3; 1.
PE 1: Evidence at protein level;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Cellulose degradation {ECO:0000256|ARBA:ARBA00023001,
KW ECO:0000256|RuleBase:RU361166};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU10059};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326,
KW ECO:0000256|PROSITE-ProRule:PRU10059};
KW Proteomics identification {ECO:0007829|PeptideAtlas:K7UN70};
KW Reference proteome {ECO:0000313|Proteomes:UP000007305};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|RuleBase:RU361166}.
FT SIGNAL 1..31
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT CHAIN 32..625
FT /note="Endoglucanase"
FT /evidence="ECO:0000256|RuleBase:RU361166"
FT /id="PRO_5011126781"
FT DOMAIN 531..611
FT /note="Carbohydrate binding"
FT /evidence="ECO:0000259|SMART:SM01063"
FT REGION 508..531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 426
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10059"
FT ACT_SITE 478
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
FT ACT_SITE 487
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10060"
SQ SEQUENCE 625 AA; 67954 MW; CEED957DAA56DEEA CRC64;
MPRLMKTSTK TPATLAVAAS FLLLLATAAG GASTPAPFDY AGAFDKCLQF FEAQRSGKLP
ADRRVHWRGD SALTDGFSQG VDLVGGYYDS GDHVKFGFPM AYAVTMLAWG VLEFEKEMVA
ANNHQRALDA IRWGTNYFVK AHTEPNSLWV QVGDGDSDHL CWERAEDMST PRTAFKINMT
HPGSEVAGET AAALAAASKA FRPYDSMYAD LLLLHAKQLF TFADTFRGRY DDSLQSTKKF
YPSGSGYQDE LLWAAAWLHE ATGDEEYLQY VSRNAEEFGG IGWSVLEFSW DNKYAGLQVL
LSKALLAGGG GEHADTLRQY QAKAEFFLCA CLQKNGGHNM KLTPGGLLHT DEWNNMQYVS
SATFLLAVYA DYLAASRGAL RCPDGEVAPG EMVRFARSQA DYVLGKNPRG MSYMVGYGSY
FPTHVHHRGA SIPSVYAMDS RVGCMDGFDR YYNSKGADPN VLHGAVVGGP DAADGFVDDR
CNYQQAEPTL AGNAPICGVF ARLASEPADA SDNSNRPAPS SHSPPSKGSP LEFVHTVSNS
WTTNGVEYYR HVVTAKNTCG HPITYLKLHV KGLSGPIYGV SAAKEKDTYE LPTWLTSLAA
GEQFTIVYIQ GGPAARFSVA SYKTA
//
