ID K8DWR9_9FIRM Unreviewed; 640 AA.
AC K8DWR9;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311};
GN ORFNames=DESHY_10042 {ECO:0000313|EMBL:CCO06882.1};
OS Desulforamulus hydrothermalis Lam5 = DSM 18033.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Peptococcaceae;
OC Desulforamulus.
OX NCBI_TaxID=1121428 {ECO:0000313|EMBL:CCO06882.1, ECO:0000313|Proteomes:UP000009315};
RN [1] {ECO:0000313|EMBL:CCO06882.1, ECO:0000313|Proteomes:UP000009315}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Lam5 / DSM 18033 {ECO:0000313|Proteomes:UP000009315};
RX PubMed=23405336; DOI=10.1128/genomeA.00114-12;
RA Amin O., Fardeau M.L., Valette O., Hirschler-Rea A., Barbe V., Medigue C.,
RA Vacherie B., Ollivier B., Bertin P.N., Dolla A.;
RT "Genome Sequence of the Sulfate-Reducing Bacterium Desulfotomaculum
RT hydrothermale Lam5(T).";
RL Genome Announc. 1:e00114-e00112(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:CCO06882.1}.
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DR EMBL; CAOS01000001; CCO06882.1; -; Genomic_DNA.
DR RefSeq; WP_008409504.1; NZ_FQXF01000039.1.
DR AlphaFoldDB; K8DWR9; -.
DR STRING; 1121428.DESHY_10042; -.
DR eggNOG; COG0749; Bacteria.
DR OrthoDB; 4053at2; -.
DR Proteomes; UP000009315; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:InterPro.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:InterPro.
DR CDD; cd06139; DNA_polA_I_Ecoli_like_exo; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR Pfam; PF01612; DNA_pol_A_exo1; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW Exonuclease {ECO:0000313|EMBL:CCO06882.1};
KW Hydrolase {ECO:0000313|EMBL:CCO06882.1};
KW Nuclease {ECO:0000313|EMBL:CCO06882.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000009315}.
FT DOMAIN 3..200
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 380..598
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 640 AA; 72762 MW; 4BCEBCA9E7319BBF CRC64;
MGYKCVYMLS EIKEYLKNTV LFAFDFETSP RDKWRNDKSA ALDAHKADIT GISFSVSEGT
AIYVPLKHRS GRNAENQAAI WDYLKLLFES KDVIKVAHNL AFESMFLYAR GIVLQKPCYD
TIAASQLTLK SKWEFRSLAD SGLKTLAPAL CKAEMTEFST VTDGRFFDEL NPQDEKTVRY
ACADSDYTLR LYHVFNQWFD RFLPKHRTIV EEVESPTSVY VGIMKYNGIL VDKSAMLKKQ
AEAAEKIVSI RKEIAGIIGN VEIGANASTS AFKKYLFVDL GLPVMKTTAK HQEAADDETM
ILLKEWCESN RPELARLFEL VQEYRKWGKL KSTYIDGYLR FIDEDTGRIH PDLMPLGTET
GRFASRNPNM QNCPQKDNDP IGIRKFIISP AGKVLLSLDF SQIELRVGAF YCRDKRMLET
YRTGGDIHAQ TTSVIYRIPF EEAADKNAPH YKERRTIAKN CNFGVFYGLF PTGLQRTLKF
KAGLNPTLSE CETIIQNLKA GYPGLAKWQD EVKKRAAVSC YTETWLGRRR YLLGIRSSDW
GKKSFAERCA LNTPIQGTAA DILKLACGRI ISGLPERLWL KPILQIHDEL VFELPEDKVD
EAVALIKECM ETQPFPEFDV PIVAEASVGR NFGEMKEMED
//
