ID K8P615_9BRAD Unreviewed; 901 AA.
AC K8P615;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=4Fe-4S Mo/W bis-MGD-type domain-containing protein {ECO:0000259|PROSITE:PS51669};
GN ORFNames=HMPREF9695_01872 {ECO:0000313|EMBL:EKS38032.1};
OS Afipia broomeae ATCC 49717.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
OC Nitrobacteraceae; Afipia.
OX NCBI_TaxID=883078 {ECO:0000313|EMBL:EKS38032.1, ECO:0000313|Proteomes:UP000001096};
RN [1] {ECO:0000313|EMBL:EKS38032.1, ECO:0000313|Proteomes:UP000001096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 49717 {ECO:0000313|EMBL:EKS38032.1,
RC ECO:0000313|Proteomes:UP000001096};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Huys G., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Afipia broomeae ATCC 49717.";
RL Submitted (APR-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=Mo-bis(molybdopterin guanine dinucleotide);
CC Xref=ChEBI:CHEBI:60539; Evidence={ECO:0000256|ARBA:ARBA00001942};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Nitrogen metabolism. {ECO:0000256|ARBA:ARBA00004909}.
CC -!- SIMILARITY: Belongs to the prokaryotic molybdopterin-containing
CC oxidoreductase family. NasA/NapA/NarB subfamily.
CC {ECO:0000256|ARBA:ARBA00008747}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKS38032.1}.
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DR EMBL; AGWX01000003; EKS38032.1; -; Genomic_DNA.
DR RefSeq; WP_006020592.1; NZ_KB375282.1.
DR AlphaFoldDB; K8P615; -.
DR PATRIC; fig|883078.3.peg.1921; -.
DR eggNOG; COG0243; Bacteria.
DR HOGENOM; CLU_000422_13_4_5; -.
DR Proteomes; UP000001096; Unassembled WGS sequence.
DR GO; GO:1990204; C:oxidoreductase complex; IEA:UniProt.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0043546; F:molybdopterin cofactor binding; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0045333; P:cellular respiration; IEA:UniProt.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR CDD; cd02791; MopB_CT_Nitrate-R-NapA-like; 1.
DR CDD; cd02754; MopB_Nitrate-R-NapA-like; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.40.50.740; -; 1.
DR Gene3D; 2.20.25.90; ADC-like domains; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.40.228.10; Dimethylsulfoxide Reductase, domain 2; 1.
DR InterPro; IPR009010; Asp_de-COase-like_dom_sf.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR041957; CT_Nitrate-R-NapA-like.
DR InterPro; IPR006657; MoPterin_dinucl-bd_dom.
DR InterPro; IPR006656; Mopterin_OxRdtase.
DR InterPro; IPR006963; Mopterin_OxRdtase_4Fe-4S_dom.
DR InterPro; IPR027467; MopterinOxRdtase_cofactor_BS.
DR PANTHER; PTHR43105:SF9; NITRATE REDUCTASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G15190)-RELATED; 1.
DR PANTHER; PTHR43105; RESPIRATORY NITRATE REDUCTASE; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF04879; Molybdop_Fe4S4; 1.
DR Pfam; PF00384; Molybdopterin; 1.
DR Pfam; PF01568; Molydop_binding; 1.
DR SMART; SM00926; Molybdop_Fe4S4; 1.
DR SUPFAM; SSF50692; ADC-like; 1.
DR SUPFAM; SSF53706; Formate dehydrogenase/DMSO reductase, domains 1-3; 1.
DR PROSITE; PS51669; 4FE4S_MOW_BIS_MGD; 1.
DR PROSITE; PS00551; MOLYBDOPTERIN_PROK_1; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Molybdenum {ECO:0000256|ARBA:ARBA00022505};
KW Nitrate assimilation {ECO:0000256|ARBA:ARBA00023063};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Reference proteome {ECO:0000313|Proteomes:UP000001096}.
FT DOMAIN 8..64
FT /note="4Fe-4S Mo/W bis-MGD-type"
FT /evidence="ECO:0000259|PROSITE:PS51669"
SQ SEQUENCE 901 AA; 96083 MW; 96418536626DF216 CRC64;
MTNVDPSVAP TKTTCPYCGV GCGVIATPDG KGGATIAGDP EHPANFGRLC SKGSALGETL
GLGERLLHPM IRCSNGKFEE VAWTDALDHV ANRLQHIIAK YGPDSVGFYL SGQLLTEDYY
VANKLMKGFI GTANVDTNSR LCMSSSVAGH RRAFGSDTVP GCYEDLDQAD LLVLVGSNAA
WCHPVLFQRM IANKQQRGAR IVVIDPRQTE TSGDADLFLG LKPGTDTALF SGLLTYLADS
GALDHSYIDE HTSGFAEGLA KARSIAGSVA ATALATGLAE TDVATFFQMF AKTPRVVTLY
SQGVNQSAQG TDKVNAIINC HLATGRIGKP GASPFSLTGQ PNAMGGREVG GLANQLAAHM
SFTPPDIDRV RRFWKAPHIA THEGLKAVQM FQAIGRGEIK ALWVMGTNPA VSLPNADAVR
AAMRKLDLLV ISENVQSNDS INSGAHVLLP AHAWGEKSGT VTNSERRISR QRAFLTPPGQ
AKPDWWIMSE VAERLGYGAA FAYHSAADIF REHAELSAFE NGGSRDFDIG AMASVADDEF
DLMPPFMWPM PKGGSEAAPR FFAEGRYYTS DRKGHFIAPD VPMLRGAISE ARPLRLNTGR
IRDQWHTMTR TGLSPRLGQH LPEPYVEIHP ADAAQADVVD GGYARLATDL GQCILKVVVS
ERQQRGMLFA PIHWSQENSA SGRVGALVAP FTDPFSGQPE NKATPVAIES VTFGQRGFVL
SRRLMSFPKD VGWSRVAVTG GYGYLIASNL DVAAWRTFLS PQGEAGHIAE YEDAAHGVYR
AAQFSGDRLE ACVFIGPAKD IPSWDAVKTA FAAETISDEQ RRLLLSGKSA DGVANDGPVV
CACFGVGRNA ICGAIAEGSR TAAEIGAKLK AGTNCGSCIP ELKRLIAQTG VAQPMEASAA
S
//
