ID   K8Z4L9_NANGC            Unreviewed;       257 AA.
AC   K8Z4L9;
DT   06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT   06-FEB-2013, sequence version 1.
DT   27-MAR-2024, entry version 26.
DE   RecName: Full=Diphthine--ammonia ligase {ECO:0000256|ARBA:ARBA00018426};
DE            EC=6.3.1.14 {ECO:0000256|ARBA:ARBA00012089};
DE   AltName: Full=Diphthamide synthase {ECO:0000256|ARBA:ARBA00029814};
DE   AltName: Full=Diphthamide synthetase {ECO:0000256|ARBA:ARBA00031552};
GN   ORFNames=NGA_2081800 {ECO:0000313|EMBL:EKU21693.1};
OS   Nannochloropsis gaditana (strain CCMP526) (Green microalga)
OS   (Microchloropsis gaditana).
OC   Eukaryota; Sar; Stramenopiles; Ochrophyta; Eustigmatophyceae;
OC   Eustigmatales; Monodopsidaceae; Nannochloropsis.
OX   NCBI_TaxID=1093141 {ECO:0000313|EMBL:EKU21693.1, ECO:0000313|Proteomes:UP000009377};
RN   [1] {ECO:0000313|Proteomes:UP000009377}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCMP526 {ECO:0000313|Proteomes:UP000009377};
RX   PubMed=22353717;
RA   Radakovits R., Jinkerson R.E., Fuerstenberg S.I., Tae H., Settlage R.E.,
RA   Boore J.L., Posewitz M.C.;
RT   "Draft genome sequence and genetic transformation of the oleaginous alga
RT   Nannochloropis gaditana.";
RL   Nat. Commun. 3:686-686(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + diphthine-[translation elongation factor 2] + NH4(+) =
CC         AMP + diphosphate + diphthamide-[translation elongation factor 2] +
CC         H(+); Xref=Rhea:RHEA:19753, Rhea:RHEA-COMP:10172, Rhea:RHEA-
CC         COMP:10174, ChEBI:CHEBI:15378, ChEBI:CHEBI:16692, ChEBI:CHEBI:28938,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:82696,
CC         ChEBI:CHEBI:456215; EC=6.3.1.14;
CC         Evidence={ECO:0000256|ARBA:ARBA00001559};
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DR   EMBL; JH471009; EKU21693.1; -; Genomic_DNA.
DR   RefSeq; XP_005854666.1; XM_005854604.1.
DR   AlphaFoldDB; K8Z4L9; -.
DR   EnsemblProtists; EKU21693; EKU21693; NGA_2081800.
DR   GeneID; 17361438; -.
DR   KEGG; ngd:NGA_2081800; -.
DR   OMA; CWHERVT; -.
DR   OrthoDB; 316736at2759; -.
DR   Proteomes; UP000009377; Unassembled WGS sequence.
DR   GO; GO:0017178; F:diphthine-ammonia ligase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   Gene3D; 3.90.1490.10; putative n-type atp pyrophosphatase, domain 2; 1.
DR   InterPro; IPR002761; Diphthami_syn_dom.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Pfam; PF01902; Diphthami_syn_2; 1.
DR   SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE   4: Predicted;
KW   Reference proteome {ECO:0000313|Proteomes:UP000009377}.
FT   DOMAIN          24..237
FT                   /note="Diphthamide synthase"
FT                   /evidence="ECO:0000259|Pfam:PF01902"
SQ   SEQUENCE   257 AA;  28134 MW;  8C39876C2B4B51BA CRC64;
     MAEDVGVSSS ISAESNGRQP SQVLLSFSGG KDSSMALHVL RNDPNFHVVG IMVQVEKDND
     RVSVHEVRRE LLDAQVAALP SDLTVFHVCL PVMSTNCQYE GAIFDCFAEA RQCFPLLKYV
     AFGDIFLADV RDYRMGVLAR WSGHGGCPLL PLFPLWGRDT TRLARDFLAL GFRAHLVCVD
     TTQLDATYVG RQYDAALLDD LPAHVDLCGE KGEFHTFVSA GPVFSSTVPV QVGHVESRGT
     AGRFAYCDLV PRARVKA
//