ID K8Z9S8_9ENTE Unreviewed; 872 AA.
AC K8Z9S8;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 63.
DE RecName: Full=DNA polymerase I {ECO:0000256|ARBA:ARBA00020311, ECO:0000256|RuleBase:RU004460};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417, ECO:0000256|RuleBase:RU004460};
GN Name=polA {ECO:0000256|RuleBase:RU004460};
GN ORFNames=C683_0274 {ECO:0000313|EMBL:EKU27809.1};
OS Catellicoccus marimammalium M35/04/3.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
OC Catellicoccus.
OX NCBI_TaxID=1234409 {ECO:0000313|EMBL:EKU27809.1, ECO:0000313|Proteomes:UP000016057};
RN [1] {ECO:0000313|EMBL:EKU27809.1, ECO:0000313|Proteomes:UP000016057}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M35/04/3 {ECO:0000313|EMBL:EKU27809.1,
RC ECO:0000313|Proteomes:UP000016057};
RX PubMed=23405330;
RA Weigand M.R., Ryu H., Bozcek L., Konstantinidis K.T., Santo Domingo J.W.;
RT "Draft Genome Sequence of Catellicoccus marimammalium, a Novel Species
RT Commonly Found in Gull Feces.";
RL Genome Announc. 1:E00019-12(2013).
CC -!- FUNCTION: In addition to polymerase activity, this DNA polymerase
CC exhibits 5'-3' exonuclease activity. {ECO:0000256|RuleBase:RU004460}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) =
CC diphosphate + DNA(n+1); Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339,
CC Rhea:RHEA-COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560,
CC ChEBI:CHEBI:173112; EC=2.7.7.7;
CC Evidence={ECO:0000256|ARBA:ARBA00024632,
CC ECO:0000256|RuleBase:RU004460};
CC -!- SUBUNIT: Single-chain monomer with multiple functions.
CC {ECO:0000256|ARBA:ARBA00011541, ECO:0000256|RuleBase:RU004460}.
CC -!- SIMILARITY: Belongs to the DNA polymerase type-A family.
CC {ECO:0000256|ARBA:ARBA00007705, ECO:0000256|RuleBase:RU004460}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKU27809.1}.
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DR EMBL; AMYT01000008; EKU27809.1; -; Genomic_DNA.
DR RefSeq; WP_009488548.1; NZ_AMYT01000008.1.
DR AlphaFoldDB; K8Z9S8; -.
DR STRING; 1234409.C683_0274; -.
DR PATRIC; fig|1234409.3.peg.245; -.
DR eggNOG; COG0258; Bacteria.
DR eggNOG; COG0749; Bacteria.
DR OrthoDB; 9806424at2; -.
DR Proteomes; UP000016057; Unassembled WGS sequence.
DR GO; GO:0008408; F:3'-5' exonuclease activity; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd08637; DNA_pol_A_pol_I_C; 1.
DR CDD; cd06140; DNA_polA_I_Bacillus_like_exo; 1.
DR CDD; cd09898; H3TH_53EXO; 1.
DR CDD; cd09859; PIN_53EXO; 1.
DR Gene3D; 3.30.70.370; -; 1.
DR Gene3D; 1.10.150.20; 5' to 3' exonuclease, C-terminal subdomain; 2.
DR Gene3D; 3.40.50.1010; 5'-nuclease; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR002562; 3'-5'_exonuclease_dom.
DR InterPro; IPR020046; 5-3_exonucl_a-hlix_arch_N.
DR InterPro; IPR002421; 5-3_exonuclease.
DR InterPro; IPR036279; 5-3_exonuclease_C_sf.
DR InterPro; IPR019760; DNA-dir_DNA_pol_A_CS.
DR InterPro; IPR001098; DNA-dir_DNA_pol_A_palm_dom.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR020045; DNA_polI_H3TH.
DR InterPro; IPR018320; DNA_polymerase_1.
DR InterPro; IPR002298; DNA_polymerase_A.
DR InterPro; IPR008918; HhH2.
DR InterPro; IPR029060; PIN-like_dom_sf.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR NCBIfam; TIGR00593; pola; 1.
DR PANTHER; PTHR10133; DNA POLYMERASE I; 1.
DR PANTHER; PTHR10133:SF62; DNA POLYMERASE THETA; 1.
DR Pfam; PF01367; 5_3_exonuc; 1.
DR Pfam; PF02739; 5_3_exonuc_N; 1.
DR Pfam; PF00476; DNA_pol_A; 1.
DR PRINTS; PR00868; DNAPOLI.
DR SMART; SM00474; 35EXOc; 1.
DR SMART; SM00475; 53EXOc; 1.
DR SMART; SM00279; HhH2; 1.
DR SMART; SM00482; POLAc; 1.
DR SUPFAM; SSF47807; 5' to 3' exonuclease, C-terminal subdomain; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF88723; PIN domain-like; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
DR PROSITE; PS00447; DNA_POLYMERASE_A; 1.
PE 3: Inferred from homology;
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|RuleBase:RU004460};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|RuleBase:RU004460};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|RuleBase:RU004460};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|RuleBase:RU004460};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|RuleBase:RU004460}; Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004460};
KW Reference proteome {ECO:0000313|Proteomes:UP000016057};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004460}.
FT DOMAIN 3..264
FT /note="5'-3' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00475"
FT DOMAIN 299..462
FT /note="3'-5' exonuclease"
FT /evidence="ECO:0000259|SMART:SM00474"
FT DOMAIN 628..836
FT /note="DNA-directed DNA polymerase family A palm"
FT /evidence="ECO:0000259|SMART:SM00482"
SQ SEQUENCE 872 AA; 99833 MW; FEC34BD90D034E1C CRC64;
MSKKVVLIDG SSLSYRAFYA LGKQVDRFVN HEGLHTNAIY SFKLMLDALL EKEKPDYWLV
AFDKSKKTFR HEQFSEYKAG RKKTPPEFLE QLPYIKELIA DQGGTVYELE GYEADDIIGT
MAQKASQEEV VIYTGDRDLF QLVTDHVTVK WTKKGVKEVE VYTPEAIFEK YQLAPKQIID
LKGLAGDTSD NIPGVTKVGE KTAIKLLTQY HSVENLYEHI DELKKSKMKE HLITDKEQAF
LSKELATIET NAPIALSLED IKTKPVATEA LIELYEKLDF KQFLKAMDRP KVAVEKTQYQ
WVDQVTAEMF HENQVVHLEL FEENYHTATP IGLAWGDQET IYVGKPDLIK EEVFQQFLAN
DEKKWFFDVK KHWVLAQKEG ITLGEKVEDI YLGSYLLDTL NDSSDVESLT EYYDVSRVAN
EEEIYGKGAK RAIPEEEILS SHLANKVKAF AEIVPKMRAQ LEEKNQMALY ETMEVPLAFV
LAKMEYRGIK VDVEQLKAQE AELGAQIKEI EEKIYAEAGH SFNLNSPKQL GEVLFDELGL
PVLKRTKTGY STSVDVLEKL EPDYPIVRDI LTYRQWSKLQ STYVTGLLKQ VHADGKVHTR
YMQGLTQTGR LSSVDPNLQN IPVRMEEGKR IRKAFVPENP DWYILTSDYS QIELRVLASI
CQDASLEQAF RDGEDIHDST ARRIFHLLPG EEVTPKMRRE AKAVNFGVVY GISDYGLSEN
LGITRKEAKA FIDQYFHHYP GVKRYMDAIV EKAKEDGYVE TLFARRRYLP DIHSRNFNVR
SFAERTAINS PIQGSAADIL KIAMNKMAKE LENSNYRAHL LLQVHDEVIL EVHPEDLEAV
QALVEKTMDE AVKLSVPLVT DSSYGKTWYD AK
//
