ID K9D954_9BURK Unreviewed; 886 AA.
AC K9D954;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=HMPREF9710_03089 {ECO:0000313|EMBL:EKU81249.1};
OS Massilia timonae CCUG 45783.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Telluria group; Massilia.
OX NCBI_TaxID=883126 {ECO:0000313|EMBL:EKU81249.1, ECO:0000313|Proteomes:UP000009874};
RN [1] {ECO:0000313|EMBL:EKU81249.1, ECO:0000313|Proteomes:UP000009874}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 45783 {ECO:0000313|EMBL:EKU81249.1,
RC ECO:0000313|Proteomes:UP000009874};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Huys G., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Massilia timonae CCUG 45783.";
RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKU81249.1}.
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DR EMBL; AGZI01000041; EKU81249.1; -; Genomic_DNA.
DR RefSeq; WP_005667883.1; NZ_JH992924.1.
DR AlphaFoldDB; K9D954; -.
DR STRING; 47229.LO55_676; -.
DR GeneID; 84810335; -.
DR PATRIC; fig|883126.3.peg.3112; -.
DR eggNOG; COG0550; Bacteria.
DR HOGENOM; CLU_002929_5_2_4; -.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000009874; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_domain.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 2.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPOISOMERASE_I_PROK; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:EKU81249.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000009874};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 3..136
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT REGION 824..886
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 886 AA; 97931 MW; AF762764B8F864F3 CRC64;
MTKSLIIAEK PSVANDIAKT LGGFTKHDEY FESDDYVLSS AVGHLLEIAV PEEHDVKRGK
WSFTHLPMIP PYFALNPIAK TESRLKVLNK LIKRKDVTTL INACDAGREG ELIFRLIAQN
AKAKQPVKRL WLQSMTANAI REGFNNLRSD EEMLPLADAA RCRSEADWLI GINGTRAMTA
FNSKEGGFYL TTVGRVQTPT LSIVVEREEK IKKFVPRDYW EVRAEFVCAA GVYEGRWLDQ
KFKKDENDPE KRAERLWSKA AADSIAAACR GKQGNVTEES KPTTSMAPGL FDLTSLQREA
NGRFGFSAKN TLGLAQALYE KHKVLTYPRT DSRHLPEDYI GTVKSTLEAL AETNNYNQFA
KQISKNGWVK PNKRIFDNTK ISDHFAIIPT GVVPKNLSEP EQKLYDLVTR RFLAVFFPAA
EFLVTTRYTE VSGHQFKTEG KVMTNPGWMA VYGKDTGDDK DGANLVPVAK GEKVLTDKVT
ANGLVTKPPA RYNEATLLSA MEGAGKLVDS DELRDAMAGK GLGTPATRAA IIEGLLTEKY
LLREGRELMP TAKAFQLMTL LRGLGVNELT APELTGEWEF KLSQMEKGKI SREEFMREIQ
QMTQIIVKRA KEYDNDTIPG EYATLVNPCP NCGGMVKENY RRFACTKCDF SMSKTPGSRQ
FEIAEVEELL KNRTIGPLQG FRSKMGRPFA AILRIVRDEE IKNFKLEFDF GQDEEGEEGE
GVDFSGQTPV GPCPKCNGGV YEMPLAYVCE HSVAKPKTCD FRSGRIILQQ EIVPEQMAKL
LNDGKTDLLP GFVSQRTRRA FKAFLVRGKD NKISFEFEER KAKPGAAAKG KAGDEAAAGA
DGADGAAAPV KAARKAAAKP AAKPAAKKAV ARKAPAKKAT AAAAKK
//
