ID K9E520_9BURK Unreviewed; 805 AA.
AC K9E520;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 24-JAN-2024, entry version 53.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:EKU84405.1};
GN ORFNames=HMPREF9710_00307 {ECO:0000313|EMBL:EKU84405.1};
OS Massilia timonae CCUG 45783.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Telluria group; Massilia.
OX NCBI_TaxID=883126 {ECO:0000313|EMBL:EKU84405.1, ECO:0000313|Proteomes:UP000009874};
RN [1] {ECO:0000313|EMBL:EKU84405.1, ECO:0000313|Proteomes:UP000009874}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 45783 {ECO:0000313|EMBL:EKU84405.1,
RC ECO:0000313|Proteomes:UP000009874};
RG The Broad Institute Genome Sequencing Platform;
RA Earl A., Ward D., Feldgarden M., Gevers D., Huys G., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Goldberg J., Griggs A., Gujja S.,
RA Hansen M., Howarth C., Imamovic A., Larimer J., McCowen C., Montmayeur A.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Massilia timonae CCUG 45783.";
RL Submitted (SEP-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKU84405.1}.
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DR EMBL; AGZI01000006; EKU84405.1; -; Genomic_DNA.
DR RefSeq; WP_005663233.1; NZ_JH992922.1.
DR AlphaFoldDB; K9E520; -.
DR STRING; 47229.LO55_977; -.
DR PATRIC; fig|883126.3.peg.307; -.
DR eggNOG; COG1024; Bacteria.
DR eggNOG; COG1250; Bacteria.
DR HOGENOM; CLU_010448_0_0_4; -.
DR OrthoDB; 5287258at2; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000009874; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR48075; 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN; 1.
DR PANTHER; PTHR48075:SF7; 3-HYDROXYACYL-COA DEHYDROGENASE-RELATED; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000009874}.
FT DOMAIN 8..198
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 201..300
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 805 AA; 86947 MW; 9916DEE5A07635BC CRC64;
MSNFIVKKVA VLGAGVMGAQ IAAHCVNAKV PVVLFDLPAK PEQGSGGPSN KNGIVLRAIE
NLKKLSPAPL GNKDDAALIE VANYEDNLDV LAGCDLIIEA IAERMDWKHD LYAKVAPHIA
PHAIFASNTS GLSINKLAEG FDAGLKERFC GVHFFNPPRY MHLVELIPTE ATRPEILDQL
ETFLTSTLGK GVVRAKDTPN FIANRVGIFG MLATIVEAEK FGLSVDVVDD LTGAKLGRAK
SGTFRTADVV GLDTMGHVIK TMQDNLKDDP FFSVYKTPDI LAKLIEKGAL GQKTGAGFYK
KVGKEIQRLD FATGEYVPSG AKAADIVGRI LKEKDPVKKF KAMRDSTNPQ AQFLWAIFRD
AFHYIAFHLD TIADNARDVD FAMRWGFGWS VGPFETWQAA GWTQIAQWVK EDIEAGKALS
DAPLPAWVFE GSVAEKGVHT PEGSYSVTKN AYVPLSDLPV YKRQQFRAPV VGLGGPDAKT
FGTTVFEDDS VRLWHSDDEV LVISLKTKMH VIGDGVIKGI QRALKEAEQG YKGIVIWNTD
AAEGGAFSAG ADLQSALPAF MAGGAKAMEP IVRELQDTFM AMKYSNIPVV AAVAGLALGG
GCELALHASK RVASIESYIG LVEVGVGLVP AGGGLKEAAL RAANEAKGND ILQFLKNGFM
NAATAQVSKS ALEAKAMGYL KEDDVIVFNA YELLHVAKTT ARAMFDAGYR APARRTFPVT
GRYGWATIRG QMVNMRDGGF ISAYDYHLGD TIAEIVTGGD IDQGSVVSEQ WILDLERKAF
VALLNNPKTQ ERIMGMMQTG KPVRN
//
