ID K9HM39_9PROT Unreviewed; 320 AA.
AC K9HM39;
DT 06-FEB-2013, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2013, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Nitrogen fixation protein NifU {ECO:0000256|ARBA:ARBA00015278, ECO:0000256|PIRNR:PIRNR000375};
GN ORFNames=C882_3791 {ECO:0000313|EMBL:EKV31418.1};
OS Caenispirillum salinarum AK4.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Rhodospirillales;
OC Novispirillaceae; Caenispirillum.
OX NCBI_TaxID=1238182 {ECO:0000313|EMBL:EKV31418.1, ECO:0000313|Proteomes:UP000009881};
RN [1] {ECO:0000313|EMBL:EKV31418.1, ECO:0000313|Proteomes:UP000009881}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK4 {ECO:0000313|EMBL:EKV31418.1,
RC ECO:0000313|Proteomes:UP000009881};
RX PubMed=23409257;
RA Khatri I., Singh A., Korpole S., Pinnaka A.K., Subramanian S.;
RT "Draft Genome Sequence of an Alphaproteobacterium, Caenispirillum salinarum
RT AK4(T), Isolated from a Solar Saltern.";
RL Genome Announc. 1:E00199-12(2013).
CC -!- FUNCTION: May be involved in the formation or repair of [Fe-S] clusters
CC present in iron-sulfur proteins. {ECO:0000256|PIRNR:PIRNR000375}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000256|ARBA:ARBA00006420,
CC ECO:0000256|PIRNR:PIRNR000375}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EKV31418.1}.
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DR EMBL; ANHY01000006; EKV31418.1; -; Genomic_DNA.
DR RefSeq; WP_009539899.1; NZ_ANHY01000006.1.
DR AlphaFoldDB; K9HM39; -.
DR STRING; 1238182.C882_3791; -.
DR PATRIC; fig|1238182.3.peg.1454; -.
DR eggNOG; COG0694; Bacteria.
DR eggNOG; COG0822; Bacteria.
DR OrthoDB; 9808097at2; -.
DR Proteomes; UP000009881; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd06664; IscU_like; 1.
DR CDD; cd19947; NifU_Fer2_BFD-like; 1.
DR Gene3D; 3.90.1010.10; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR016217; N_fixation_NifU.
DR InterPro; IPR010238; NIF_FeS_clus_asmbl_NifU.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR NCBIfam; TIGR02000; NifU_proper; 1.
DR PANTHER; PTHR10093:SF8; IRON-SULFUR CLUSTER ASSEMBLY ENZYME ISCU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR10093; IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01106; NifU; 1.
DR Pfam; PF01592; NifU_N; 1.
DR PIRSF; PIRSF000375; NifU; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF82649; SufE/NifU; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR000375-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000375-1};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|PIRNR:PIRNR000375};
KW Reference proteome {ECO:0000313|Proteomes:UP000009881}.
FT DOMAIN 4..125
FT /note="NIF system FeS cluster assembly NifU N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01592"
FT DOMAIN 137..185
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 248..311
FT /note="NIF system FeS cluster assembly NifU C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01106"
FT BINDING 35
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 62
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 106
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 138
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 140
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 173
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 176
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
SQ SEQUENCE 320 AA; 33771 MW; 39EFC415A2750F82 CRC64;
MWNYTDKVKE YFFDPRNAGV LEDANGVGEV GSISCGDALR LMIKVDETTG LITDARFQTF
GCGSAIASSS ALTELIIGKT VDEARAVTNQ DIADFLGGLP PEKMHCSVMG HEALIAAIAN
YKGEELAEDD HEEGALLCKC FGIDEGMVER AVKGNKLTTL EQVTAYTKAG GSCKTCHEKI
EEALATFNAQ MVEEGTLSAA EAFTLGDAPK PAPKPATVSG PVQISPLAEQ ATKAKPSGLT
NLQKITLIQQ AIEEIRPQLQ RDGGDISLVD VDGDTVVVQL SGTCVGCQMS AVTIQGVQAA
LMEKVGRPLR VIPHTAMASA
//
