ID KCNJ8_MOUSE Reviewed; 424 AA.
AC P97794;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 24-JAN-2024, entry version 178.
DE RecName: Full=ATP-sensitive inward rectifier potassium channel 8;
DE AltName: Full=Inward rectifier K(+) channel Kir6.1;
DE AltName: Full=Potassium channel, inwardly rectifying subfamily J member 8;
DE AltName: Full=uKATP-1;
GN Name=Kcnj8;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Brain;
RX PubMed=9130167; DOI=10.1113/jphysiol.1997.sp021963;
RA Yamada M., Isomoto S., Matsumoto S., Kondo C., Shindo T., Horio Y.,
RA Kurachi Y.;
RT "Sulphonylurea receptor 2B and Kir6.1 form a sulphonylurea-sensitive but
RT ATP-insensitive K+ channel.";
RL J. Physiol. (Lond.) 499:715-720(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-6, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: This potassium channel is controlled by G proteins. Inward
CC rectifier potassium channels are characterized by a greater tendency to
CC allow potassium to flow into the cell rather than out of it. Their
CC voltage dependence is regulated by the concentration of extracellular
CC potassium; as external potassium is raised, the voltage range of the
CC channel opening shifts to more positive voltages. The inward
CC rectification is mainly due to the blockage of outward current by
CC internal magnesium. Can be blocked by external barium (By similarity).
CC {ECO:0000250|UniProtKB:Q63664}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the inward rectifier-type potassium channel (TC
CC 1.A.2.1) family. KCNJ8 subfamily. {ECO:0000305}.
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DR EMBL; D88159; BAA13550.1; -; mRNA.
DR EMBL; BC003756; AAH03756.1; -; mRNA.
DR CCDS; CCDS20685.1; -.
DR RefSeq; NP_001317292.1; NM_001330363.1.
DR RefSeq; NP_032454.1; NM_008428.5.
DR AlphaFoldDB; P97794; -.
DR SMR; P97794; -.
DR STRING; 10090.ENSMUSP00000145440; -.
DR iPTMnet; P97794; -.
DR PhosphoSitePlus; P97794; -.
DR PaxDb; 10090-ENSMUSP00000032374; -.
DR ProteomicsDB; 268960; -.
DR ABCD; P97794; 2 sequenced antibodies.
DR Antibodypedia; 4014; 279 antibodies from 31 providers.
DR DNASU; 16523; -.
DR Ensembl; ENSMUST00000203945.3; ENSMUSP00000145440.2; ENSMUSG00000030247.10.
DR GeneID; 16523; -.
DR KEGG; mmu:16523; -.
DR UCSC; uc009epk.1; mouse.
DR AGR; MGI:1100508; -.
DR CTD; 3764; -.
DR MGI; MGI:1100508; Kcnj8.
DR VEuPathDB; HostDB:ENSMUSG00000030247; -.
DR eggNOG; KOG3827; Eukaryota.
DR GeneTree; ENSGT00990000203615; -.
DR HOGENOM; CLU_022738_4_0_1; -.
DR InParanoid; P97794; -.
DR OMA; ISEEIQW; -.
DR OrthoDB; 4126787at2759; -.
DR PhylomeDB; P97794; -.
DR TreeFam; TF313676; -.
DR Reactome; R-MMU-1296025; ATP sensitive Potassium channels.
DR BioGRID-ORCS; 16523; 1 hit in 77 CRISPR screens.
DR ChiTaRS; Kcnj8; mouse.
DR PRO; PR:P97794; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; P97794; Protein.
DR Bgee; ENSMUSG00000030247; Expressed in brain blood vessel and 176 other cell types or tissues.
DR ExpressionAtlas; P97794; baseline and differential.
DR Genevisible; P97794; MM.
DR GO; GO:0098978; C:glutamatergic synapse; ISO:MGI.
DR GO; GO:0008282; C:inward rectifying potassium channel; ISO:MGI.
DR GO; GO:0030016; C:myofibril; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0098793; C:presynapse; IDA:MGI.
DR GO; GO:0048787; C:presynaptic active zone membrane; ISO:MGI.
DR GO; GO:0032991; C:protein-containing complex; IDA:MGI.
DR GO; GO:0042383; C:sarcolemma; ISO:MGI.
DR GO; GO:0005524; F:ATP binding; ISO:MGI.
DR GO; GO:0015272; F:ATP-activated inward rectifier potassium channel activity; ISO:MGI.
DR GO; GO:0019829; F:ATPase-coupled monoatomic cation transmembrane transporter activity; ISO:MGI.
DR GO; GO:0017098; F:sulfonylurea receptor binding; ISO:MGI.
DR GO; GO:0099508; F:voltage-gated monoatomic ion channel activity involved in regulation of presynaptic membrane potential; ISO:MGI.
DR GO; GO:1902282; F:voltage-gated potassium channel activity involved in ventricular cardiac muscle cell action potential repolarization; ISO:MGI.
DR GO; GO:0001508; P:action potential; IMP:MGI.
DR GO; GO:0002250; P:adaptive immune response; IMP:MGI.
DR GO; GO:0006915; P:apoptotic process; IMP:MGI.
DR GO; GO:0060922; P:atrioventricular node cell differentiation; IMP:MGI.
DR GO; GO:0001568; P:blood vessel development; IMP:MGI.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IMP:MGI.
DR GO; GO:0061762; P:CAMKK-AMPK signaling cascade; IMP:MGI.
DR GO; GO:0060920; P:cardiac pacemaker cell differentiation; IMP:MGI.
DR GO; GO:0008283; P:cell population proliferation; IMP:MGI.
DR GO; GO:0072359; P:circulatory system development; IMP:MGI.
DR GO; GO:0060976; P:coronary vasculature development; IMP:MGI.
DR GO; GO:0051607; P:defense response to virus; IMP:MGI.
DR GO; GO:0008340; P:determination of adult lifespan; IGI:MGI.
DR GO; GO:0030010; P:establishment of cell polarity; IDA:MGI.
DR GO; GO:0045444; P:fat cell differentiation; IMP:MGI.
DR GO; GO:0015908; P:fatty acid transport; IMP:MGI.
DR GO; GO:0048144; P:fibroblast proliferation; IGI:MGI.
DR GO; GO:0010467; P:gene expression; IMP:MGI.
DR GO; GO:0061535; P:glutamate secretion, neurotransmission; IGI:MGI.
DR GO; GO:0007507; P:heart development; IMP:MGI.
DR GO; GO:0003007; P:heart morphogenesis; IMP:MGI.
DR GO; GO:0006954; P:inflammatory response; IMP:MGI.
DR GO; GO:0098662; P:inorganic cation transmembrane transport; ISO:MGI.
DR GO; GO:0001822; P:kidney development; IEA:Ensembl.
DR GO; GO:0098915; P:membrane repolarization during ventricular cardiac muscle cell action potential; ISO:MGI.
DR GO; GO:0001774; P:microglial cell activation; IMP:MGI.
DR GO; GO:0050877; P:nervous system process; IMP:MGI.
DR GO; GO:0050905; P:neuromuscular process; IMP:MGI.
DR GO; GO:0044546; P:NLRP3 inflammasome complex assembly; IDA:MGI.
DR GO; GO:0038066; P:p38MAPK cascade; IMP:MGI.
DR GO; GO:0010087; P:phloem or xylem histogenesis; IMP:MGI.
DR GO; GO:1990573; P:potassium ion import across plasma membrane; ISO:MGI.
DR GO; GO:0071805; P:potassium ion transmembrane transport; IMP:MGI.
DR GO; GO:0006813; P:potassium ion transport; ISO:MGI.
DR GO; GO:0009306; P:protein secretion; IMP:MGI.
DR GO; GO:0150103; P:reactive gliosis; IMP:MGI.
DR GO; GO:0008217; P:regulation of blood pressure; IMP:MGI.
DR GO; GO:0002027; P:regulation of heart rate; IMP:MGI.
DR GO; GO:0034765; P:regulation of monoatomic ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0033198; P:response to ATP; IMP:MGI.
DR GO; GO:0034097; P:response to cytokine; IMP:MGI.
DR GO; GO:0034976; P:response to endoplasmic reticulum stress; IMP:MGI.
DR GO; GO:0043330; P:response to exogenous dsRNA; IMP:MGI.
DR GO; GO:0001666; P:response to hypoxia; IMP:MGI.
DR GO; GO:0032868; P:response to insulin; IMP:MGI.
DR GO; GO:0002931; P:response to ischemia; IMP:MGI.
DR GO; GO:0032496; P:response to lipopolysaccharide; IMP:MGI.
DR GO; GO:1904638; P:response to resveratrol; IMP:MGI.
DR GO; GO:0009410; P:response to xenobiotic stimulus; IMP:MGI.
DR GO; GO:0051124; P:synaptic assembly at neuromuscular junction; IMP:MGI.
DR GO; GO:0019226; P:transmission of nerve impulse; IMP:MGI.
DR GO; GO:0001944; P:vasculature development; IMP:MGI.
DR GO; GO:0042311; P:vasodilation; IMP:MGI.
DR GO; GO:0003229; P:ventricular cardiac muscle tissue development; IGI:MGI.
DR Gene3D; 1.10.287.70; -; 1.
DR Gene3D; 2.60.40.1400; G protein-activated inward rectifier potassium channel 1; 1.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR041647; IRK_C.
DR InterPro; IPR016449; K_chnl_inward-rec_Kir.
DR InterPro; IPR003278; K_chnl_inward-rec_Kir6.1.
DR InterPro; IPR013518; K_chnl_inward-rec_Kir_cyto.
DR InterPro; IPR040445; Kir_TM.
DR PANTHER; PTHR11767:SF11; ATP-SENSITIVE INWARD RECTIFIER POTASSIUM CHANNEL 8; 1.
DR PANTHER; PTHR11767; INWARD RECTIFIER POTASSIUM CHANNEL; 1.
DR Pfam; PF01007; IRK; 1.
DR Pfam; PF17655; IRK_C; 1.
DR PIRSF; PIRSF005465; GIRK_kir; 1.
DR PRINTS; PR01331; KIR61CHANNEL.
DR PRINTS; PR01320; KIRCHANNEL.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF81324; Voltage-gated potassium channels; 1.
PE 1: Evidence at protein level;
KW Ion channel; Ion transport; Membrane; Phosphoprotein; Potassium;
KW Potassium transport; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..424
FT /note="ATP-sensitive inward rectifier potassium channel 8"
FT /id="PRO_0000154948"
FT TOPO_DOM 1..69
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 70..94
FT /note="Helical; Name=M1"
FT /evidence="ECO:0000250"
FT TOPO_DOM 95..126
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT INTRAMEM 127..138
FT /note="Helical; Pore-forming; Name=H5"
FT /evidence="ECO:0000250"
FT INTRAMEM 139..145
FT /note="Pore-forming"
FT /evidence="ECO:0000250"
FT TOPO_DOM 146..154
FT /note="Extracellular"
FT /evidence="ECO:0000250"
FT TRANSMEM 155..176
FT /note="Helical; Name=M2"
FT /evidence="ECO:0000250"
FT TOPO_DOM 177..424
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT REGION 373..409
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 140..145
FT /note="Selectivity filter"
FT /evidence="ECO:0000250"
FT COMPBIAS 390..409
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 170
FT /note="Role in the control of polyamine-mediated channel
FT gating and in the blocking by intracellular magnesium"
FT /evidence="ECO:0000250"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 424 AA; 47980 MW; BF02D1CC31970379 CRC64;
MLARKSIIPE EYVLARIAAE NLRKPRIRDR LPKARFIAKS GACNLAHKNI REQGRFLQDI
FTTLVDLKWR HTLVIFTMSF LCSWLLFAIM WWLVAFAHGD IYAYMEKGTM EKSGLESAVC
VTNVRSFTSA FLFSIEVQVT IGFGGRMMTE ECPLAITVLI LQNIVGLIIN AVMLGCIFMK
TAQAHRRAET LIFSRHAVIA VRNGKLCFMF RVGDLRKSMI ISASVRIQVV KKTTTPEGEV
VPIHQQDIPV DNPIESNNIF LVAPLIICHV IDKRSPLYDI SATDLANQDL EVIVILEGVV
ETTGITTQAR TSYIAEEIQW GHRFVSIVTE EEGVYSVDYS KFGNTVRVAA PRCSARELDE
KPSILIQTLQ KSELSHQNSL RKRNSMRRNN SMRRNNSIRR NNSSLMVPKV QFMTPEGNQC
PSES
//
