ID KSL7_ORYSI Reviewed; 830 AA.
AC Q00G37; A2X6A1;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 22-FEB-2023, entry version 71.
DE RecName: Full=Ent-cassa-12,15-diene synthase;
DE EC=4.2.3.28;
DE AltName: Full=Diterpene cyclase 1;
DE Short=OsDTC1;
DE AltName: Full=Ent-kaurene synthase-like 7;
DE Short=OsKSL7;
GN Name=KSL7; Synonyms=DTC1; ORFNames=OsI_007594;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. IR24;
RX PubMed=16956633; DOI=10.1016/j.phytochem.2006.08.009;
RA Peters R.J.;
RT "Uncovering the complex metabolic network underlying diterpenoid
RT phytoalexin biosynthesis in rice and other cereal crop plants.";
RL Phytochemistry 67:2307-2317(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP FUNCTION.
RX PubMed=17141283; DOI=10.1016/j.phytochem.2006.10.016;
RA Xu M., Wilderman P.R., Morrone D., Xu J., Roy A., Margis-Pinheiro M.,
RA Upadhyaya N.M., Coates R.M., Peters R.J.;
RT "Functional characterization of the rice kaurene synthase-like gene
RT family.";
RL Phytochemistry 68:312-326(2007).
CC -!- FUNCTION: Involved in phytocassane phytoalexins biosynthesis. Catalyzes
CC the conversion of ent-copalyl diphosphate to the phytoalexin precursor
CC ent-cassa-12,15-diene. {ECO:0000269|PubMed:17141283}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ent-copalyl diphosphate = diphosphate + ent-cassa-12,15-diene;
CC Xref=Rhea:RHEA:25532, ChEBI:CHEBI:33019, ChEBI:CHEBI:50060,
CC ChEBI:CHEBI:58553; EC=4.2.3.28;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC Note=Binds 3 Mg(2+) ions per subunit. {ECO:0000250};
CC -!- TISSUE SPECIFICITY: Expressed in roots and stems.
CC -!- DEVELOPMENTAL STAGE: Expressed from 2 to 6 days after imbibition.
CC -!- INDUCTION: By chitin oligosaccharide elicitor and UV irradiation.
CC -!- DOMAIN: The Asp-Asp-Xaa-Xaa-Asp/Glu (DDXXD/E) motif is important for
CC the catalytic activity, presumably through binding to Mg(2+).
CC -!- MISCELLANEOUS: Ent-cassa-12,15-diene is a precursor of the phytoalexins
CC phytocassanes A-E. Phytoalexins are diterpenoid secondary metabolites
CC involved in the defense mechanism of the plant and produced in response
CC to attack (by a pathogen, elicitor or UV irradiation).
CC -!- SIMILARITY: Belongs to the terpene synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAY86361.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ823354; ABH10734.1; -; mRNA.
DR EMBL; CM000127; EAY86361.1; ALT_INIT; Genomic_DNA.
DR AlphaFoldDB; Q00G37; -.
DR SMR; Q00G37; -.
DR STRING; 39946.Q00G37; -.
DR HOGENOM; CLU_003125_2_0_1; -.
DR Proteomes; UP000007015; Chromosome 2.
DR GO; GO:0034277; F:ent-cassa-12,15-diene synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0000287; F:magnesium ion binding; IEA:InterPro.
DR GO; GO:0010333; F:terpene synthase activity; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0016102; P:diterpenoid biosynthetic process; IEA:InterPro.
DR CDD; cd00684; Terpene_cyclase_plant_C1; 1.
DR Gene3D; 1.50.10.160; -; 1.
DR Gene3D; 1.10.600.10; Farnesyl Diphosphate Synthase; 1.
DR Gene3D; 1.50.10.130; Terpene synthase, N-terminal domain; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR044814; Terpene_cyclase_plant_C1.
DR InterPro; IPR001906; Terpene_synth_N.
DR InterPro; IPR036965; Terpene_synth_N_sf.
DR InterPro; IPR005630; Terpene_synthase_metal-bd.
DR InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR PANTHER; PTHR31739; ENT-COPALYL DIPHOSPHATE SYNTHASE, CHLOROPLASTIC; 1.
DR PANTHER; PTHR31739:SF3; ENT-KAUR-16-ENE SYNTHASE, CHLOROPLASTIC; 1.
DR Pfam; PF01397; Terpene_synth; 1.
DR Pfam; PF03936; Terpene_synth_C; 1.
DR SFLD; SFLDG01014; Terpene_Cyclase_Like_1_N-term; 1.
DR SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2.
DR SUPFAM; SSF48576; Terpenoid synthases; 1.
PE 2: Evidence at transcript level;
KW Lyase; Magnesium; Metal-binding; Plant defense; Reference proteome.
FT CHAIN 1..830
FT /note="Ent-cassa-12,15-diene synthase"
FT /id="PRO_0000372321"
FT REGION 1..50
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 577..581
FT /note="DDXXD motif"
FT BINDING 577
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 577
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 581
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="1"
FT /evidence="ECO:0000250"
FT BINDING 581
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="2"
FT /evidence="ECO:0000250"
FT BINDING 721
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT BINDING 729
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /ligand_label="3"
FT /evidence="ECO:0000250"
FT CONFLICT 829
FT /note="S -> L (in Ref. 1; ABH10734)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 830 AA; 92180 MW; CE5C635B0DD9AE45 CRC64;
MMLLGSPSSG GYGGKFAGAS PAGGTTTMAP SAKQPSSRAP PPGITGGRND LRILSPAAAA
AAVGGLEMKK PEAEGIAESL QATHRKELEA SIRKQLQGVE LSPSPYDTAW VAMVPLRGSS
HNPSFPQCVD WILENQWDDG SWSIDGSIST ANKDVLSSTL ACVLALNKWN VGREHIRRGL
SFIGRNFSIA MDDQAVAPIG FGITFPAMLT LANGSGLEVP VRQNDIDSLN HLREMKIQRE
AGNHSRGRKA YMAYLAEGFG NLLEWDEIMM FQRKNGSLFN CPSSTAGALA NYHDDKALQY
LQSLVNKFDG VVPTLYPLNI YCQLSMVDAL ENMGISQYFA SEIKSILDMT YSSWLGRDEE
IMLDVTTCAM AFRLLRMNGY DVSSDELSHV AGASGFRDSL QGYLNDRKSV LEVYKTSKHS
ISENDLILDS IGSWSGSLLK EMLCSNGIQG TPGREEIEFA LKYPFYSTLE RLVHRKNIVL
FDAKGSQMLK TECMPVHDSQ DFLALAVDDF CISQSNYQNE LNYLESWVKD NRLDQLHFAR
QKITYCYLSG AATTFRPEMG YARTSWARTA WLTAVIDDLF DVGGLEQEQE NLLALMEKWE
EPGEDEYYSE DVKIVFQALY NTVNEIGAKA SALQGHDVTK YLVDVWLHVV RCMKVEAEWQ
RSQHLPTFEE YMESGMVSLG QGATVMSALF LIGEKLPEGV VELEEYDEMF RLMGTCGRLL
NDIRGIEREE SDGKMTNGVS LLVHASGGSM SVDEAKTEVM KRIDASRRKL LSLVVGEQEG
PIPRPCKQLF WKMCKILHLF YYQTDGFSSP KEMVSAVDAV IKEPLQLRSL
//
