ID L0EFA0_THECK Unreviewed; 581 AA.
AC L0EFA0;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN OrderedLocusNames=Theco_2194 {ECO:0000313|EMBL:AGA58314.1};
OS Thermobacillus composti (strain DSM 18247 / JCM 13945 / KWC4).
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Thermobacillus.
OX NCBI_TaxID=717605 {ECO:0000313|EMBL:AGA58314.1, ECO:0000313|Proteomes:UP000010795};
RN [1] {ECO:0000313|Proteomes:UP000010795}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18247 / JCM 13945 / KWC4
RC {ECO:0000313|Proteomes:UP000010795};
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Teshima H., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Anderson I.,
RA Woyke T.;
RT "Complete sequence of chromosome of Thermobacillus composti KWC4.";
RL Submitted (JAN-2012) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
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DR EMBL; CP003255; AGA58314.1; -; Genomic_DNA.
DR RefSeq; WP_015255059.1; NC_019897.1.
DR AlphaFoldDB; L0EFA0; -.
DR STRING; 717605.Theco_2194; -.
DR KEGG; tco:Theco_2194; -.
DR eggNOG; COG1293; Bacteria.
DR HOGENOM; CLU_022481_2_1_9; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000010795; Chromosome.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.8.50; -; 1.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000010795};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 460..548
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT COILED 303..330
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
FT COILED 388..437
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 581 AA; 65375 MW; 07ADD5F949DB4895 CRC64;
MSLDGIVVRA IAHELRQLAG TRIHRIHQPD DHTLVLQLRG GGLRGQSRLL LSANPTYPRV
HWVTRPQPNP LEAPMFCMLM RKHCEGGVIE TVTQPGCERV LHIDIRQRDE LGDISLKRIV
VELMGRHSNI ILTDPATGRI YDGIHHVTPA ISSYRVVMPG SAYTPPPEQA KADPLAVPDF
ARFAESLRPA EGDSPPDRRL VDAYAGISPL LAREIVHRAA AADAPDGTEA LWRAFRDVME
GFRSHGYEPN IVDRDGRTFF SVTRLTHLDG DVRTFDSILE CLEIYYGDKA ERDLVRQKSA
DLHRFLLNEK TKNEKKIEKL EATLEEAKEA DRYRITGELI IANMHAIQKG QPYVEVIDYY
DERQPVVTIE LDPQLTPSEN AQRWFKRYNK AKNSITAAAA QMEAAREEIA YFDTLLAQLE
TASLQDIEEI REELAEQGYL KQRGGAGAKK RKPSRPALLC YTSSENVPIY VGKNNTQNEY
LTNRLASPND TWLHTKDIPG SHVVIRGTGF GNATLEEAAM LAAYYSRGKE SSLVPVDYTL
IRHVRKPSGA KPGFVIYDHQ KTLFVTPDEE RIKALPVRTV T
//
