ID L0HHG9_METFS Unreviewed; 304 AA.
AC L0HHG9;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=Tyrosine recombinase XerA {ECO:0000256|HAMAP-Rule:MF_02055};
GN Name=xerA {ECO:0000256|HAMAP-Rule:MF_02055};
GN OrderedLocusNames=Metfor_2491 {ECO:0000313|EMBL:AGB03485.1};
OS Methanoregula formicica (strain DSM 22288 / NBRC 105244 / SMSP).
OC Archaea; Euryarchaeota; Stenosarchaea group; Methanomicrobia;
OC Methanomicrobiales; Methanoregulaceae; Methanoregula.
OX NCBI_TaxID=593750 {ECO:0000313|EMBL:AGB03485.1, ECO:0000313|Proteomes:UP000010824};
RN [1] {ECO:0000313|Proteomes:UP000010824}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22288 / NBRC 105244 / SMSP
RC {ECO:0000313|Proteomes:UP000010824};
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Ovchinnikova G., Teshima H., Detter J.C., Han C., Tapia R.,
RA Land M., Hauser L., Kyrpides N., Ivanova N., Pagani I., Imachi H.,
RA Tamaki H., Sekiguchi Y., Kamagata Y., Cadillo-Quiroz H., Zinder S.,
RA Liu W.-T., Woyke T.;
RT "Complete sequence of Methanoregula formicicum SMSP.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AGB03485.1, ECO:0000313|Proteomes:UP000010824}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 22288 / NBRC 105244 / SMSP
RC {ECO:0000313|Proteomes:UP000010824};
RX PubMed=25189582;
RA Yamamoto K., Tamaki H., Cadillo-Quiroz H., Imachi H., Kyrpides N.,
RA Woyke T., Goodwin L., Zinder S.H., Kamagata Y., Liu W.T.;
RT "Complete Genome Sequence of Methanoregula formicica SMSPT, a Mesophilic
RT Hydrogenotrophic Methanogen Isolated from a Methanogenic Upflow Anaerobic
RT Sludge Blanket Reactor.";
RL Genome Announc. 2:e00870-14(2014).
CC -!- FUNCTION: Site-specific tyrosine recombinase, which acts by catalyzing
CC the cutting and rejoining of the recombining DNA molecules.
CC {ECO:0000256|HAMAP-Rule:MF_02055}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_02055}.
CC -!- SIMILARITY: Belongs to the 'phage' integrase family. XerA subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_02055}.
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DR EMBL; CP003167; AGB03485.1; -; Genomic_DNA.
DR RefSeq; WP_015286447.1; NC_019943.1.
DR AlphaFoldDB; L0HHG9; -.
DR STRING; 593750.Metfor_2491; -.
DR GeneID; 14309883; -.
DR KEGG; mfo:Metfor_2491; -.
DR eggNOG; arCOG01241; Archaea.
DR HOGENOM; CLU_027562_9_0_2; -.
DR InParanoid; L0HHG9; -.
DR OrthoDB; 142231at2157; -.
DR Proteomes; UP000010824; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009037; F:tyrosine-based site-specific recombinase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006313; P:DNA transposition; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.150.130; -; 1.
DR Gene3D; 1.10.443.10; Intergrase catalytic core; 1.
DR HAMAP; MF_02055; Recomb_XerA; 1.
DR InterPro; IPR044068; CB.
DR InterPro; IPR011010; DNA_brk_join_enz.
DR InterPro; IPR013762; Integrase-like_cat_sf.
DR InterPro; IPR002104; Integrase_catalytic.
DR InterPro; IPR010998; Integrase_recombinase_N.
DR InterPro; IPR004107; Integrase_SAM-like_N.
DR InterPro; IPR033686; XerA.
DR PANTHER; PTHR30349:SF41; INTEGRASE_RECOMBINASE HI_1414-RELATED; 1.
DR PANTHER; PTHR30349; PHAGE INTEGRASE-RELATED; 1.
DR Pfam; PF13495; Phage_int_SAM_4; 1.
DR Pfam; PF00589; Phage_integrase; 1.
DR SUPFAM; SSF56349; DNA breaking-rejoining enzymes; 1.
DR PROSITE; PS51900; CB; 1.
DR PROSITE; PS51898; TYR_RECOMBINASE; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|HAMAP-Rule:MF_02055};
KW DNA integration {ECO:0000256|ARBA:ARBA00022908, ECO:0000256|HAMAP-
KW Rule:MF_02055};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172, ECO:0000256|HAMAP-
KW Rule:MF_02055};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_02055}; Reference proteome {ECO:0000313|Proteomes:UP000010824}.
FT DOMAIN 4..108
FT /note="Core-binding (CB)"
FT /evidence="ECO:0000259|PROSITE:PS51900"
FT DOMAIN 129..294
FT /note="Tyr recombinase"
FT /evidence="ECO:0000259|PROSITE:PS51898"
FT ACT_SITE 164
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 188
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 246
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 249
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 272
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
FT ACT_SITE 281
FT /note="O-(3'-phospho-DNA)-tyrosine intermediate"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_02055"
SQ SEQUENCE 304 AA; 35828 MW; 4EF545B6C037A4F0 CRC64;
MESGYFSEWL KSYRNYLRMR NYSPRTLDSY EQVIRHFGYY VWLRRNMDVT KLVFHWKDFE
NARLDTSVDV TPVMVNDFLA FVSSMQTYKP KTFHRIISTL SSFYRFLSTQ GVVSINPLLG
IDRPRIKQQE IKYLKHNQVL RLIDSIDDLR DKLIVRTIYA TGVRVSELCN MNIEDIDFDE
HTIRIRGKGD KIRLVFVDED TLAEILSFIG TRTSGPLFVG QQGKHISSRA IQHIFKHYAP
SGITPHKIRH SYASELYKRS KNLRVVQENL GHTSIKTTEI YLHTDIEERR QVYQQYFPLS
GSNE
//
