ID L0J6M5_9MYCO Unreviewed; 811 AA.
AC L0J6M5;
DT 06-MAR-2013, integrated into UniProtKB/TrEMBL.
DT 06-MAR-2013, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE RecName: Full=site-specific DNA-methyltransferase (adenine-specific) {ECO:0000256|ARBA:ARBA00011900};
DE EC=2.1.1.72 {ECO:0000256|ARBA:ARBA00011900};
GN ORFNames=Mycsm_06103 {ECO:0000313|EMBL:AGB26262.1};
OS Mycobacterium sp. JS623.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=212767 {ECO:0000313|EMBL:AGB26262.1, ECO:0000313|Proteomes:UP000010844};
RN [1] {ECO:0000313|Proteomes:UP000010844}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JS623 {ECO:0000313|Proteomes:UP000010844};
RG US DOE Joint Genome Institute;
RA Lucas S., Han J., Lapidus A., Cheng J.-F., Goodwin L., Pitluck S.,
RA Peters L., Teshima H., Detter J.C., Han C., Tapia R., Land M., Hauser L.,
RA Kyrpides N., Ivanova N., Pagani I., Mattes T., Holmes A., Rutledge P.,
RA Paulsen I., Coleman N., Woyke T.;
RT "Complete sequence of chromosome of Mycobacterium smegmatis JS623.";
RL Submitted (OCT-2011) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an
CC N(6)-methyl-2'-deoxyadenosine in DNA + H(+) + S-adenosyl-L-
CC homocysteine; Xref=Rhea:RHEA:15197, Rhea:RHEA-COMP:12418, Rhea:RHEA-
CC COMP:12419, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:90615, ChEBI:CHEBI:90616; EC=2.1.1.72;
CC Evidence={ECO:0000256|ARBA:ARBA00001279};
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DR EMBL; CP003078; AGB26262.1; -; Genomic_DNA.
DR RefSeq; WP_015309836.1; NC_019966.1.
DR AlphaFoldDB; L0J6M5; -.
DR STRING; 212767.Mycsm_06103; -.
DR REBASE; 58775; M.Msm623ORF6103P.
DR KEGG; msa:Mycsm_06103; -.
DR PATRIC; fig|710686.3.peg.6148; -.
DR HOGENOM; CLU_013049_6_0_11; -.
DR OrthoDB; 9784823at2; -.
DR Proteomes; UP000010844; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0008170; F:N-methyltransferase activity; IEA:InterPro.
DR GO; GO:0009007; F:site-specific DNA-methyltransferase (adenine-specific) activity; IEA:UniProtKB-EC.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1260.30; -; 2.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR022749; D12N6_MeTrfase_N.
DR InterPro; IPR003356; DNA_methylase_A-5.
DR InterPro; IPR002052; DNA_methylase_N6_adenine_CS.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR InterPro; IPR038333; T1MK-like_N_sf.
DR PANTHER; PTHR42933; SLR6095 PROTEIN; 1.
DR PANTHER; PTHR42933:SF3; TYPE I RESTRICTION ENZYME MJAVIII METHYLASE SUBUNIT; 1.
DR Pfam; PF12161; HsdM_N; 1.
DR Pfam; PF02384; N6_Mtase; 1.
DR PRINTS; PR00507; N12N6MTFRASE.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS00092; N6_MTASE; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603,
KW ECO:0000313|EMBL:AGB26262.1};
KW Restriction system {ECO:0000256|ARBA:ARBA00022747};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AGB26262.1}.
FT DOMAIN 10..122
FT /note="N6 adenine-specific DNA methyltransferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF12161"
FT DOMAIN 135..442
FT /note="DNA methylase adenine-specific"
FT /evidence="ECO:0000259|Pfam:PF02384"
SQ SEQUENCE 811 AA; 89648 MW; 679BFEBCA926839A CRC64;
MALKKSDLYS SLWKSCDELR GGMDASQYKD YILTLLFVKY VSDKAKADPT SLIEVPTGGS
FDDLVKLKGK PNIGEEINVA IRKLADANDL QGVINNADFD DPNKLGDGKA MQDRLSKLIG
IFQALDFSGS RAEGDDLLGD AYEYLMRHFA TESGKSKGQF YTPAEVSRVM AQVIGIKPNT
PKRTTVYDPT CGSGSLLLKV VDAAPNGLTI YGQEKDNATW ALSKMNMILH GNVTADIRMG
DSIVDPKFRS GDTLDSFDLL VANPPFSVKS WTNGLENEYG RFDGYARPPE KNGDYAFLLH
MVKSLKSTGK GAVILPHGVL FRGHTEATIR KELIKRGYIK GIIGLPANLF YGTGIPACII
VLDKENAQAR TGIFMIDASK GFMKDGNKNR LRPRDMHQIV DSFTKQIEVE RYSRMVPLKE
IADPNNDYNL NIPRYIDSSE PEDIQDLHAH LHGGIPDRDL DALDDYWKAF STLRSTLFTE
NRPGYSDLTI DVASVQETVL DSAKFKKFAA RVDADVDEWL GSHRARLAKI TENTKPGELI
SEISEDLLAR FKSVPLLDEY DVYEQLMTYW NETMHDDVFL IMNEGWVGAA KPRKTIEDKD
RKLTEVPDLI AGSGRGATKY KMDLIPPAVM VAANFADYQP YIDELTAAAE EAARAVEEYT
EEHAVEDGLL AAAMDDDKIS KALAAARLKD AKKEGSDPDE IKALQHLIDL YDAETDAKKA
VKEAQAELDA AVLKEYGHLT EDAVKSLVLD DKWCAAVRGR IGGEVNSLTL DLVARIQELG
ERYSETVGAL DAELERLEAT IAGHLADMGV N
//
